THE CELLULAR AND HUMORAL IMMUNITY STATE DEPENDING ON THE OUTCOME OF A WIDESPREAD PURULENT PERITONITIS

Cover Page


Cite item

Full Text

Abstract

The aim of the study was to examine the state of cellular and humoral immunity in patients with widespread purulent peritonitis (WPP) in depending on the disease outcome. The study involved 50 patients with community-acquired and hospital origin WPP. The testing of blood lymphocyte phenotype was performed by flow cytometry. The concentration of immunoglobulins and cytokines were measured by ELISA. It was established that the immune system state by WPP is characterized by leukocytosis, relative lymphopenia, increasing concentrations of pro-inflammatory cytokines, as well as decrease of the cytotoxic and activated T-lymphocytes content. The state of the cellular immunity in case of unfavorable outcome of the WPP is characterized by decreased of the γδT-lymphocytes and NKT-cells levels with increasing amounts of the B1-cells. The immune system state in case of a favorable outcome of the WPP is characterized by a decrease in the number of NK-cells in peripheral blood and increased levels of Th2-lymphocytes. Increasing of the Th2-cells number determines the increase in stimulating effects of T-cell on the humoral immunity, that is manifested in increasing concentrations of IL-4 and IgA, which is an important factor in the immunopathogenesis of the WPP determining its favorable outcome. 

About the authors

A. A. Savchenko

Scientific Research Institute of Medical Problems of the North, Krasnoyarsk, Russian Federation

Author for correspondence.
Email: fake@neicon.ru

PhD, MD (Medicine), Professor, Head of the Laboratory of Molecular-Cell Physiology and Pathology, Scientific Research Institute of Medical Problems of the North, Krasnoyarsk, Russian Federation;

Russian Federation

A. G. Borisov

Scientific Research Institute of Medical Problems of the North, Krasnoyarsk, Russian Federation

Email: fake@neicon.ru

PhD (Medicine), Leading Researcher of the Laboratory of Molecular-Cell Physiology and Pathology, Scientific Research Institute of Medical Problems of the North, Krasnoyarsk, Russian Federation;

Russian Federation

D. E. Zdzitoveckij

Krasnoyarsk State Medical University, Krasnoyarsk, Russian Federation

Email: fake@neicon.ru

PhD, MD (Medicine), Head of the Department of Surgical Diseases, Krasnoyarsk State Medical University, Krasnoyarsk, Russian Federation;

Russian Federation

I. V. Kudryavtsev

197376, Russian Federation, St. Petersburg, Acad. Pavlova str., 12, Scientific Research Institute of Experimental Medicine.
Far Eastern Federal University, Vladivostok, Russian Federation

Email: igprek1981@yandex.ru

PhD (Biology), Senior Researcher of the Department of Immunology, Research Institute
of Experimental Medicine, St. Petersburg; Senior Researcher of the Biomedical School, Far Eastern Federal University, Vladivostok, Russian Federation. 

Russian Federation

References

  1. Борисов А.Г. Клиническая характеристика нарушения функции иммунной системы // Медицинская иммунология. 2013. Т. 15, No 1. С. 45–50. [Borisov A.G. Clinical characteristics of dysfunction of the immune system. Meditsinskaya immunologiya = Medical Immunology (Russia), 2013, vol. 15, no. 1, pp. 45–50. (In Russ.)]
  2. Земсков А.М., Земсков В.М. Дополнительные методы оценки иммунного статуса // Клиническая лабораторная диагностика. 1994. No 3. С. 34–35. [Zemskov A.M., Zemskov V.M. Additional methods for assessing of the immune status. Klinicheskaya laboratornaya diagnostika = Clinical Laboratory Diagnostics, 1994, no. 3, pp. 34–35. (In Russ.)]
  3. Савельев В.С., Филимонов М.И., Гельфанд Б.Р., Подачин П.В., Бурневич С.З., Гиткович В.Е., Гайнулин Ш.М. Программируемый перитонеальный лаваж в лечении распространенного перитонита // Анналы хирургии. 1996. No 3. С. 25–29 [Savel’ev V.S., Filimonov M.I., Gel’fand B.R., Podachin P.V., Burnevich S.Z., Gitkovich V.E., Gajnulin S.M. Programmable peritoneal lavage in the treatment of widespread peritonitis. Annaly khirurgii = Annals of Surgery, 1996, no. 3, pp. 25–29. (In Russ.)]
  4. Савченко А.А., Здзитовецкий Д.Э., Борисов А.Г., Лузан Н.А. Особенности состояния клеточного и гуморального иммунитета и уровни концентрации цитокинов у больных с распространенным гнойным перитонитом // Сибирское медицинское обозрение. 2013. No 1 (79). С. 24–28. [Savchenko A.A., Zdzitoveckij D.E., Borisov A.G., Luzan N.A. Features of the cellular and humoral immunity state and cytokine concentrations in patients with widespread purulent peritonitis. Sibirskoe meditsinskoe obozrenie = Siberian Medical Review, 2013, no. 1 (79), pp. 24–28. (In Russ.)]
  5. Сарап П.В., Винник Ю.С., Останин А.А. Влияние иммунотропной терапии на структуру системообразующих показателей у пациентов с ургентной хирургической патологией // Вестник хирургии им. И.И. Грекова. 2012. Т. 171, No 4. С. 39–43. [Sarap P.V., Vinnik Ju.S., Ostanin A.A. Immunotropic influence on the structure of the backbone of therapy parameters in patients with urgent surgical pathology. Vestnik khirurgii im. I.I. Grekova = Herald of Surgery named after I.I. Grekov, 2012, vol. 171, no. 4, pp. 39–43. (In Russ.)]
  6. Суковатых Б.С., Блинков Ю.Ю., Букреева А.Е., Ештокин С.А., Иванов П.А., Жуковский В.А. Применение иммобилизованных форм гипохлорита натрия в комплексном лечении распространенного гнойного перитонита // Вестник хирургии им. И.И. Грекова. 2011. Т. 170, No 6. С. 32–36. [Sukovatyh B.S., Blinkov Ju.Ju., Bukreeva A.E., Eshtokin S.A., Ivanov P.A., Zhukovskij V.A. The use of immobilized forms of sodium hypochlorite in the complex treatment of widespread purulent peritonitis. Vestnik khirurgii im. I.I. Grekova = Herald of Surgery named after I.I. Grekov, 2011, vol. 170, no. 6, pp. 32–36. (In Russ.)]
  7. Хайдуков С.В., Байдун Л.А., Зурочка А.В., Тотолян Арег А. Стандартизованная технология «Исследование субпопуляционного состава лимфоцитов периферической крови с применением проточных цитофлюориметрованализаторов» (Проект) // Медицинская иммунология. 2012. Т. 14, No 3. С. 255–268. [Hajdukov S.V., Bajdun L.A., Zurochka A.V., Totoljan Areg A. Standardized technology «Research of lymphocytes subpopulation composition in peripheral blood using flow cytometry analyzers» (Draft). Meditsinskaya immunologiya = Medical Immunology (Russia), 2012, vol. 14, no. 3, pp. 255–268. (In Russ.)]
  8. Ярилин А.А. Иммунология. М: ГЭОТАР-Медиа, 2010. 752 с. [Jarilin A.A. Immunologiya [Immunology]. Moscow: GEOTARMedia, 2010. 752 p.]
  9. Amezcua Vesely M.C., Schwartz M., Bermejo D.A., Montes C.L., Cautivo K.M., Kalergis A.M., Rawlings D.J., AcostaRodríguez E.V., Gruppi A. FcγRIIb and BAFF differentially regulate peritoneal B1 cell survival. J. Immunol., 2012, vol. 188, no. 10, pp. 4792–4800.
  10. Bone R.S., Balk R.A.B., Cerra F.B. American college of Chest Physicians. Society of Critical Care Medicine Consensus Conference: Definitions for sepsis and organ failure and guide lines for the use of innovative therapies in sepsis. Crit. Care Med., 1992, vol. 20, no. 6, pp. 864–874.
  11. Carter C.R., Aravind G., Smalle N.L., Cole J.Y., Savic S., Wood P.M. CVID patients with autoimmunity have elevated T cell expression of granzyme B and HLA-DR and reduced levels of Treg cells. J. Clin. Pathol., 2013, vol. 66, no. 2, pp. 146–150.
  12. Griffin D.O., Rothstein T.L. Human B1 cell frequency: isolation and analysis of human B1 cells. Front. Immunol., 2012, vol. 3, pp. 122–123.
  13. Griveas I., Fleva A., Karanikas E., Gogos K., Sakellariou G. CD4/CD8 T-cell ratio in peritoneal dialysis effluents predicts the outcome of peritonitis in patients undergoing continuous ambulatory peritoneal dialysis. Artif. Organs., 2009, vol. 33, no. 12, pp. 1091–1095.
  14. Harris N., Gause W.C. To B or not to B: B cells and the Th2-type immune response to helminthes. Trends Immunol., 2011, vol. 32, no. 2, pp. 80–88.
  15. KiankC.,EntleutnerM.,FürllB.,WesterholtA.,HeideckeC.D.,SchüttC.Stress-inducedimmuneconditioningaffectsthecourse of experimental peritonitis. Shock, 2007, vol. 27, no. 3, pp. 305–311.
  16. Kleinnijenhuis J., Quintin J., Preijers F., Joosten L.A., Jacobs C., Xavier R.J., Van Der Meer J.W., Van Crevel R., Netea M.G. BCG-induced trained immunity in NK cells: role for non-specific protection to infection. Clin. Immunol., 2014, vol. 155, no. 2, pp. 213–219.
  17. Le Gall J.-R., Lemeshow S., Saulnier F. A new simplified acute physiology score (SAPS II) based on a European/North American multicenter study. JAMA, 1993, vol. 270, pp. 2957–2963.
  18. Lertworapreecha M., Patumraj S., Niruthisard S., Hansasuta P., Bhattarakosol P. Cytotoxic function of gamma delta (gamma/ delta) T cells against pamidronate-treated cervical cancer cells. Indian J. Exp. Biol., 2013, vol. 51, no. 8, pp. 597–605.
  19. Linder M.M., Wacha H., Feldmann U., Wesch G., Streifensand R.A., Gundlach E. Der Mannheimer Peritonitis-Index. Ein Instrument zur intraoperativen Prognose der Peritonitis. Chirurg, 1987, no. 58, pp. 84–91.
  20. Luider J., Cyfra M., Johnson P., Auer I. Impact of the new Beckman Coulter Cytomics FC 500 5-color flow cytometer on a regional flow cytometry clinical laboratory service. Lab. Hematol., 2004, vol. 10, pp. 102–108.
  21. Maecker H., McCoy P., Nussenblatt R. Standardizing immunophenotyping for the human immunology project. Nat. Rev. Immunol., 2012, vol. 12, pp. 191–200.
  22. MarçaisA.,WalzerT.mTOR:agatetoNKcellmaturationandactivation.CellCycle,2014,vol.13,no.21,pp.3315–3316.
  23. McKee S.J., Mattarollo S.R., Leggatt G.R. Immunosuppressive roles of natural killer T (NKT) cells in the skin. J. Leukoc. Biol., 2014, vol. 96, no. 1, pp. 49–54.
  24. 24. PillaiM.R.,BixM.EvolutionofIL4andpathogenantagonism.GrowthFactors,2011,vol.29,no.4,pp.153–160.
  25. SistaF.,SchietromaM.,SantisG.D.,MatteiA.,CeciliaE.M.,PiccioneF.,LeardiS.,CarleiF.,AmicucciG.Systemicinflammation and immune response after laparotomy vs laparoscopy in patients with acute cholecystitis, complicated by peritonitis. World J. Gastrointest. Surg., 2013, vol. 5, no. 4, pp. 73–82.
  26. Terabe M., Berzofsky J.A. The immunoregulatory role of type I and type II NKT cells in cancer and other diseases. Cancer Immunol. Immunother., 2014, vol. 63, no. 3, pp. 199–213.
  27. VasudevA.,YingC.T.,AyyadhuryS.,PuanK.J.,AndiappanA.K.,NyuntM.S.,ShadanN.B.,MustafaS.,LowI.,RotzschkeO., Fulop T., Ng T.P., Larbi A. γ/δ T cell subsets in human aging using the classical α/β T cell model. J. Leukoc. Biol., 2014, vol. 96, no. 4, pp. 647–655.
  28. VincentJ.L.,MorenoR.,TakalaJ.,WillattsS.,DeMendonçaA.,BruiningH.,ReinhartC.K.,SuterP.M.,ThijsL.G.TheSOFA (Sepsis-related Organ Failure Assessment) score to describe organ dysfunction/failure. On behalf of the Working Group on SepsisRelated Problems of the European Society of Intensive Care Medicine. Intensive Care Med., 1996, vol. 22, no. 7, pp. 707–710.
  29. WiestR.,KragA.,GerbesA.Spontaneousbacterialperitonitis:recentguidelinesandbeyond.Gut,2012,vol.61,no.2,pp.297–310.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2015 Savchenko A.A., Borisov A.G., Zdzitoveckij D.E., Kudryavtsev I.V.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 64788 от 02.02.2016.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies