SEROLOGICAL DIAGNOSIS OF MODERN CHOLERA USING LIPOSOMAL ENTEROTOXIC DIAGNOSTICUM IN COMPLEMENT FIXATION TEST

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Abstract

Abstract. The possibility of serological diagnosis of cholera using cholera enterotoxic diagnostics kit in complement fixation test to detect anti-enterotoxic antibodies in sera of patients with cholera caused by hybrid variants of the El Tor biovar has been demonstrated. In patients with mild course of cholera anti-enterotoxic antibodies were detected in titres 1:50 and 1:200 in paired sera obtained on the 7th and 14th days of disease, respectively (fourfold titre increase). In patients with the course of medium severity 32-fold titre increase was recorded from the titre 1:100 in serum obtained on the fifth day of disease till the titre 1:3200 — on the twelfth day of disease. Antibodies titers reached 1:1600 and 1:800 were revealed in two medium course patients (adult and infant of 10 months) on the sixth day of disease.

About the authors

I. V. Savelyeva

The Federal Government Public Health Institution “Stavropol Institute for Plague Control of the Rospotrebnadzor”, Stavropol

Author for correspondence.
Email: snipchi@mail.stv.ru

PhD (Medicine), Senior Research Associate, Laboratory of media for cultivation of microorganisms of I–IV groups of pathogenicity

355035, Russian Federation, Stavropol, Sovetskaya Street, 13–15

 

Russian Federation

V. E. Bezsmertnyj

The Federal Government Public Health Institution “Anti-plague Centre of the Rospotrebnadzor”, Moscow

Email: snipchi@mail.stv.ru
PhD (Medicine), Director Russian Federation

V. N. Savelyev

The Federal Government Public Health Institution “Stavropol Institute for Plague Control of the Rospotrebnadzor”, Stavropol

Email: snipchi@mail.stv.ru
PhD, MD (Medicine), Senior Research Associate, Chief, Laboratory for Diagnosis of Cholera and Other Intestinal Infections Russian Federation

Yu. M. Fedorov

The Federal Government Public Health Institution “Anti-plague Centre of the Rospotrebnadzor”, Moscow

Email: snipchi@mail.stv.ru
PhD, MD (Medicine), Professor, Deputy Director Russian Federation

C. M. Ivanova

The Federal Government Public Health Institution “Anti-plague Centre of the Rospotrebnadzor”, Moscow

Email: snipchi@mail.stv.ru
Deputy Director Russian Federation

V. V. Ivannikov

The Federal Government Public Health Institution “Anti-plague Centre of the Rospotrebnadzor”, Moscow

Email: snipchi@mail.stv.ru
Chief, Bacteriological Laboratory Russian Federation

References

  1. Савельева И.В. Разработка липосомального холерного энтеротоксического диагностикума // Холера и патогенные для человека вибрионы. — Ростов н/Д., 2001. — Вып. № 14. — С. 84–86. Savel`eva I.V. Razrabotka liposomal`nogo kholernogo enterotoksicheskogo diagnostikuma [The development of liposomal cholera enterotoxic diagnostics]. Holera i patogennye dlya cheloveka vibriony [Cholera and Pathogenic Vibrio for Human]. Rostov on Don, 2001, no. 14, pp. 84–86.
  2. Савельева И.В. Новый диагностикум для серологической диагностики холеры // Материалы VIII съезда Всерос. о-ва эпидемиологов, микробиологов и паразитологов, 26–28 марта 2002 г. — М., 2002. — Т. 3. — С. 322–323. Savel`eva I.V. Novyy diagnostikum dlya serologicheskoy diagnostiki kholery [The new reagent for serological diagnostics of cholera]. Materialy VIII s”ezda Vseros. o-va epidemiologov, mikrobiologov i parazitologov, 26–28 marta 2002 [Proc. V III meeting of Russian Society of epidemiol. microbiol. parazitol.]. Moscow, 2002, vol. 3, pp. 322–323.
  3. Смирнова Н.И., Заднова С.П., Шашкова А.В., Кутырев В.В. Вариабельность генома измененных вариантов Vibrio choleraе Эль Тор, завезенных на территорию России в современный период // Молекулярная генетика, микробиология и вирусология. — 2011. — № 4. — С. 11–18. Smirnova N.I., Zadnova S.P., Shashkova A.V., Kutyrev V.V. Variabel`nost` genoma izmenennykh variantov Vibrio cholerae El` Tor, zavezennykh na territoriyu Rossii v sovremennyy period [Variability of genome of changed variants of Vibrio choleraе El Tor imported to the territory of Russia in modern period]. Molekulyarnaya Genetika, Mikrobiologiya i Virusologiya — Molecular Genetics, Microbiology and Virology, 2011, no. 4, pp. 11–18.
  4. Шашкова А.В., Краснов Я.М. Генотипы измененных вариантов Vibrio choleraе биовара Эль Тор, выделенных на территории России // Материалы III науч.-практ. школы-конф. молодых ученых и специалистов науч.-исследоват. организаций Роспотребнадзора. — Протвино: ЗАО «А-ПРИНТ», 2011. — С. 145–147. Shashkova A.V., Krasnov Ya.M. Genotipy izmenennykh variantov Vibrio cholerae biovara El` Tor, vydelennykh na territorii Rossii [Genotypes of changed variants of Vibrio choleraе El Tor isolated in the territory of Russia]. Materialy III nauch.-prakt. shkoly-konf. molodykh uchenykh i spetsialistov nauch.-issledovat. organizatsiy Rospotrebnadzora [Proc. III scientific practical school and conference of young researches and specialists of research institutions of Rospotrebnadzor]. Protvino: A-PRINT, 2011, pp. 145–147.

Copyright (c) 2014 Savelyeva I.V., Bezsmertnyj V.E., Savelyev V.N., Fedorov Y.M., Ivanova C.M., Ivannikov V.V.

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