THE SEARCH OF OPTIMAL COMBINATION OF ANTIGENS FOR SEROLOGICAL DIAGNOSTICS OF TUBERCULOSIS

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Abstract

Abstract. The four chimeric recombinant antigens CBD-CFP10, CBD-ESAT6, ESAT6-CFP10 and CBD-P38 contained aminoacid sequences of full-size proteins ESAT6, CFP10 and matured protein P38 of M. tuberculosis, joined with aminoacid sequences of cellulose bind domain of endogluconase A (CBD) from Cellumonas fimi have been obtained by gene engineering methods. Recombinant proteins were purified by affine chromatography in column with Ni-NTA-sepharose 6В-CL and as PPDN-3 were used for detection of their antigenic activity in indirect ELISA for TB serological diagnostics. The sera from patients with lung tuberculosis (n = 321), from persons who had professional contacts with TB patients (n = 42), from healthy blood donors (n = 366) and from patients with lung diseases of non-TB etiology were tested. It was detected that there was positive correlation between antibodies level for all studied antigens compared by pair. It has been demonstrated that although antigens were different by antigenic and immunobiological characteristics they add each other in the content of antigenic diagnostics compositions. Thus, all these antigens can be used in the test kits for serological diagnostics of TB. Using of these antigens will allow to detect persons infected by TB and patients with active tuberculosis. 

About the authors

E. V. Vasilyeva

Saint Petersburg Pasteur Institute, Saint Petersburg

Author for correspondence.
Email: ev_vasilyeva@pasteurorg.ru

Junior Research Associate, Department of New Technologies, Laboratory of Biological Preparations

Mira str. 14, St. Petersburg, 197101, Russian Federation

 

Россия

V. N. Verbov

Saint Petersburg Pasteur Institute, Saint Petersburg

Email: ev_vasilyeva@pasteurorg.ru
PhD (Chemistry), Chief, New Technologies Department Россия

A. B. Beklemishev

Research Institute for Biochemistry of Siberian Branch of the Russian Academy of Medical Sciences, Novosibirsk

Email: ev_vasilyeva@pasteurorg.ru
PhD, MD (Biology), Professor, Chief, Laboratory of Genetic Engineering Россия

A. L. Mamaev

Research Institute for Biochemistry of Siberian Branch of the Russian Academy of Medical Sciences, Novosibirsk

Email: ev_vasilyeva@pasteurorg.ru
Junior Research Associate Россия

A. O. Tsyrul’nikov

Research Institute for Biochemistry of Siberian Branch of the Russian Academy of Medical Sciences, Novosibirsk

Email: ev_vasilyeva@pasteurorg.ru

Junior Research Associate 

Россия

T. A. Stepanenko

City Multiprofile hospital No. 2, St. Petersburg

Email: ev_vasilyeva@pasteurorg.ru
PhD (Medicine), Head of the Department of Pulmonology No. 2 Россия

V. B. Ivanovskii

City Tuberculosis Dispensary, St. Petersburg

Email: ev_vasilyeva@pasteurorg.ru
PhD (Medicine), Deputy Chief Physician on Medical Work Россия

S. V. Lapin

Pavlov State Medical University, St. Petersburg

Email: ev_vasilyeva@pasteurorg.ru
PhD (Medicine), Chief, Laboratory of Autoimmune Diagnostics, Center for Molecular Medicine Россия

Areg A. Totolian

Saint Petersburg Pasteur Institute, Saint Petersburg

Email: ev_vasilyeva@pasteurorg.ru
PhD, MD (Medicine), Corresponding Member of the RAMS, Deputy Director on Scientific Work Россия

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Copyright (c) 2014 Vasilyeva E.V., Verbov V.N., Beklemishev A.B., Mamaev A.L., Tsyrul’nikov A.O., Stepanenko T.A., Ivanovskii V.B., Lapin S.V., Totolian A.A.

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