Cover Page

Cite item


Abstract. Streptococcus agalactiae can cause variety of diseases of newborns and adults. For successful colonization of different human tissues and organs as well as for suppression of the host immune system S. agalactiae expresses numerous virulence factors. For coordinated expression of the virulence genes S. agalactiae employs regulatory molecules including regulatory proteins of two-component systems. Results of the present study demonstrated that in S. agalactiae strain A49V the natural mutation in the brgR gene encoding for BgrR regulatory protein, which is component of regulatory system BgrRS, resulted in the repression of Bac protein synthesis, a virulence factor of S. agalactiae. A single nucleotide deletion in the bgrR gene has caused a shift of the reading frame and the changes in the primary, secondary and tertiary structures of the BgrR protein. The loss of functional activity of BgrR protein in A49V strain and repression of Bac protein synthesis have increased virulence of the strain in experimental animal streptococcal infection.

About the authors

A. S. Rozhdestvenskaya

ФГБУ НИИ экспериментальной медицины СЗО РАМН, Санкт-Петербург

Russian Federation

I. Santos-Sanches

Центр микробиологии, Университет «Нова», Лиссабон


A. V. Dmitriev

ФГБУ НИИ экспериментальной медицины СЗО РАМН, Санкт-Петербург

Author for correspondence.

д.б.н., заместитель директора

197376, Санкт-Петербург, ул. акад. Павлова, 12

Russian Federation


  1. Дмитриев А.В., Рождественская А.С., Зуткис А.А., Тотолян А.А. Направленная регуляция патогенных свойств стрептококков // Мед. Акад. Журн. — 2009. — № 4. — С. 50–58.
  2. Дмитриев А.В., Hu Y.Y., Shen A.D., Суворов А.Н., Yang Y.H. Анализ клинических штаммов стрептококков группы В методами молекулярной генетики // Мед. Акад. Журн. — 2002. — № 2. — С. 18–27.
  3. Маниатис Т., Фрич Э., Сэмбрук Дж. Методы генетической инженерии. Молекулярное клонирование / пер. с англ. под ред. А.А. Баева и К.Г. Скрябина. — М.: Мир, 1984. — 479 с.
  4. Рождественская А.С., Bhide M., Mikula I. Jr., Mikula I., Дмитриев А.В. Использование полиморфиз ма гена sak0192 Streptococcus agalactiae в качестве молекулярно-эпидемиологического маркера // Журнал микробиологии, эпидемиологии и иммунобиологии. — 2009. — № 5. — С. 38–43.
  5. Тотолян А.А., Суворов А.Н., Дмитриев А.В. Стрептококки группы В в патологии человека / под общ. ред. А.А. Тотоляна // СПб.: Человек. — 2009. — 212 с.
  6. Areschoug T., Stalhammar-Carlemalm M., Karlsson I., Lindahl G. Streptococcal β protein has separate binding sites for human factor H and IgA-Fc // J. Biol. Chem. — 2002. — Vol. 277. — P. 12642–12648.
  7. Berardi A., Tzialla C., Riva M., Cerbo R.M., Creti R. Group B streptococcus: early- and late-onset infections // J. Chemother. — 2007. — Vol. 19. — P. 24–27.
  8. Cox K.H., Ruiz-Bustos E., Courtney H.S. Inactivation of DltA modulates virulence factor expression in Streptococcus pyogenes // PLoS ONE. — 2009. — Vol. 4. — e5366.
  9. Dmitriev A., Hu Y.Y., Shen A.D., Suvorov A., Yang Y.H. Chromosomal analysis of group B streptococcal clinical strains; bac gene-positive strains are genetically homogenous // FEMS Microbiol. Lett. — 2002. — Vol. 208. — P. 93–98.
  10. Dmitriev A., Yang Y.H., Shen A.D., Totolian A.A. Adjacent location of bac gene and two-component regulatory system genes within the putative Streptococcus agalactiae pathogenicity island // Folia Microbiol. — 2006. — Vol. 51. — P. 229–235.
  11. Gao R., Stock A.M. Biological insights from structures of two-component proteins // Annu. Rev. Microbiol. — 2009. — Vol. 63. — P. 133–154.
  12. Heden L.-O., Frithz E., Lindahl G. Molecular characterization of an IgA receptor from group B streptococci: sequence of the gene, identification of a proline-rich region with unique structure and isolation of N-terminal fragments with IgA-binding capacity // Eur. J. Immunol. — 1991. — Vol. 21. — P. 1481–1490.
  13. Jiang S.-M., Cieslewicz M.J., Kasper D.L., Wessels M.R. Regulation of virulence by a two-component system in group B streptococcus // J. Bacteriol. — 2005. — Vol. 187. — P. 1105–1113.
  14. Jordan S., Hutchings M.I., Mascher T. Cell envelope stress response in gram-positive bacteria // FEMS Microbiol. Rev. — 2008. — Vol. 32. — P. 107–146.
  15. Larsen J.W., Sever J.L. Group B streptococcus and pregnancy: a review // Am. J. Obstet. Gynecol. — 2008. — Vol. 198. — P. 440–448.
  16. Lindahl G., Stalhammar-Carlemalm M., Areschoug T. Surface proteins of Streptococcus agalactiae and related proteins in other bacterial pathogens // Clin. Microbiol. Rev. — 2005. — Vol. 18. — P. 102–127.
  17. Nordström T., Movert E., Olin A.I., Ali S.R., Nizet V., Varki A., Areschoug T. Human Siglec-5 in-hibitory receptor and immunoglobulin A (IgA) have separate binding sites in streptococcal beta protein // J. Biol. Chem. — 2011. — Vol. 286. — P. 33981–33991.
  18. Perry J., Koteva K., Wright G. Receptor domains of two-component signal transduction systems // Mol. Biosyst. — 2011. — Vol. 7. — P. 1388–1398.
  19. Rajagopal L. Understanding the regulation of group B streptococcal virulence factors // Future Microbiol. — 2009. — Vol. 4. — P. 201–221.
  20. Rozhdestvenskaya A.S., Totolian A.A., Dmitriev A.V. Inactivation of DNA-binding response regulator Sak189 abrogates beta-antigen expression and affects virulence of Streptococcus agalactiae // PLoS ONE. — 2010. — Vol. 5. — e10212.
  21. Santi I., Grifantini R., Jiang S.-M., Brettoni C., Grandi G., Wessels M.R., Soriani M. CsrRS regulates group B streptococcus virulence gene expression in response to environmental pH: a new perspective on vaccine development // J. Bacteriol. — 2009. — Vol. 191. — P. 5387–5397.
  22. Sendi P., Johansson L., Norrby-Teglund A. Invasive group B streptococcal disease in non-pregnant adults: a review with emphasis on skin and soft-tissue infections // Infection. — 2008. — Vol. 36. — P. 100–111.
  23. Stock A.M., Robinson V.L., Goudreau P.N. Two-component signal transduction // Annu. Rev. Biochem. — 2000. — Vol. 69. — P. 183–215.

Copyright (c) 2014 Rozhdestvenskaya A.S., Santos-Sanches I., Dmitriev A.V.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 64788 от 02.02.2016.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies