Cellular immune response in convalescents from Ixodes tick-borne borreliosis

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Abstract

Ixodes tick-borne borreliosis is a natural focal transmissive infectious multi-system disease featured with complex pathogenesis, which multiple aspects remain unclarified. The persistence stage during this infection associated with prolonged Borrelia presence in metastatic foci and repeated multiple dissemination is most complicated for clinical practice. It is assumed that the chronic process can be caused by an inadequate immune response associated with activated autoimmune mechanisms leading to emergence of permanent irreversible (degenerative and atrophic) changes in affected organs. Patients who experienced tick-borne borreliosis need dynamic observation for assessing disease prognosis providing a maintenance therapy. The purpose of the study was to evaluate an opportunity of using cellular immunity indices for predicting disease transition to a chronic course.

Materials and methods. The study was carried out at the Medical Association of the Far East Branch of the Russian Academy of Sciences. Case report form data, serum and blood plasma samples collected from 22 patients aged 29–83 years old were examined. Immunological examination data from patients with ixodes tick-borne borreliosis (12–18 months after the onset of acute period) were analyzed. Specific IgM and IgG against Borrelia burgdorferi were determined by using the OMNICS diagnostic test system (St. Petersburg). Lymphocyte immunophenotyping was performed on BD FACSCalibur flow cytometer (Becton Dickinson, USA) by using doublelabeled monoclonal antibodies (Beckman Coulter, France).

Results. A variability of activated peripheral blood lymphocytes was found in patients with tick-borne borreliosis evidencing about individual immune response. An imbalanced cellular immune response recorded in seronegative convalescents from tick-borne borreliosis, may be an indirect finding of ongoing Borrelia infection. Finding of specific serum IgG and IgM antibodies in convalescents at late stage coupled to impaired immune system is a warning sign presuming a risk to developing autoimmune reactions. Detection of specific IgM antibodies at late timepoint combined with increased immune cytotoxic potential may be referred to a marker of possible disease transition to chronic course.

About the authors

T. S. Zaporozhets

Somov Institute of Epidemiology and Microbiology

Author for correspondence.
Email: niiem_vl@mail.ru
ORCID iD: 0000-0002-8879-8496

Tatiyana S. Zaporozhets, PhD, MD (Medicine), Senior Researcher, Deputy Director for the Science

690087, Vladivostok, Selskaya str., 1.

Phone: +7 (423) 244-24-34. Fax: +7 (423) 244-14-38.

Россия

S. A. Sokotun

Pacific State Medical University

Email: sokotun.s@mail.ru

PhD (Medicine), Assistant Professor, Department of Infectious Diseases

Vladivostok 

Россия

A. I. Simakova

Pacific State Medical University

Email: anna-inf@yandex.ru

PhD, MD (Medicine), Professor, Head of the Department of Infectious Diseases

Vladivostok

Россия

E. V. Persiyanova

Somov Institute of Epidemiology and Microbiology; Medical Department of Far East Branch of the Russian Academy of Sciences

Email: helen-pers@yandex.ru

PhD (Biology), Senior Researcher; Head of Laboratory of Innovative Medical and Biological Research and Technologies

Vladivostok

Россия

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Copyright (c) 2020 Zaporozhets T.S., Sokotun S.A., Simakova A.I., Persiyanova E.V.

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