COMPARATIVE CHARACTERISTICS OF INTESTINE MICROBIOME OF REPUBLIC OF GUINEA AND RUSSIAN FEDERATION RESIDENTS

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Abstract

The gut microbiota of healthy people, living in different regions, may vary considerably. The article presents the results of comparative qualitative and quantitative study of the intestine aerobic and anaerobic microbiota of residents of the Republic of Guinea (46) and Russia (60). The content of microorganisms (Enterobacteriaceae, Enterococcus spp., Lactobacillus spp., Bifidobacterium spp., Staphylococcus spp., Candida spp., non-fermentative gram-negative bacteria and others) in 1.0 gram of faeces was determined by bacteriological methods. Generic and species identification was performed using Vitek 2 Compact (bioMerieux, France), tube biochemical tests and MALDI-TОF mass spectrometry (Bruker Daltonics, Germany). Pearson χ2 criterion, p < 0.05 and Fisher’s exact test (medstatistica.ru) were used to assess the differences in the compared groups. 95% confidence intervals were calculated by the method of Wilson. Microbiological disorders were characterized by a decrease in the number of obligate microorganisms, an increase in the number of facultative (opportunistic) microorganisms (above 106 CFU/g) and the emergence of their associations. Dysbiotic disorders were identified in both compared groups: in 100% patients from Republic of Guinea (95% CI:92.3–100) and in 86.7% patients from St. Petersburg (95% CI:75.8–93.1). Severe degree of microbiota disorders in the residents of the Republic of Guinea was revealed at 19.6% (95% CI:10.7–33.2), in group of residents of St. Petersburg in 9.6% (95% CI:4.2–20.6). In both groups the microbiota disorders of third degree were detected less frequently, compared with the microbiota disorders of second degree. The study has found no significant differences in the content of obligate bacteria (Bifidobacterium spp. and Lactobacillus spp.), however, significant differences in the species composition of the facultative part of the microbiota were revealed. The residents of the Republic of Guinea had “atypical” E. coli (lactosonegative and hemolytic) and non-fermenting gram-negative bacteria Comamonas kerstersii more frequently. Opportunistic microorganism associations have been found in 30.4% of the residents of the Republic of Guinea (95% CI:19.1–44.8) and 18.3% of residents of St. Petersburg (95% CI:10.6–29.9). Opportunistic microorganism associations from the residents of the Republic of Guinea always contained Staphylococcus aureus. It is necessary to conduct further research on a bigger population to access the differences in the compared groups in Staphylococcus aureus and Hafnia alvei.

 

About the authors

A. Yu. Popova

Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Moscow

Email: kaflidia@mail.ru
PhD, MD (Medicine), Professor, Chief State Sanitary Physician of the Russian Federation, Head Россия

L. A. Kaftyreva

St. Petersburg Pasteur Institute, St. Petersburg

Author for correspondence.
Email: kaflidia@mail.ru
PhD, MD (Medicine), Professor, Head of the Laboratory of Enteric Infections, St. Petersburg Pasteur Institute Россия

L. V. Suzhaeva

St. Petersburg Pasteur Institute, St. Petersburg

Email: kaflidia@mail.ru
Researcher, Laboratory of Enteric Infections Россия

E. V. Voitenkova

St. Petersburg Pasteur Institute, St. Petersburg

Email: kaflidia@mail.ru
PhD (Medicine), Senior Researcher, Laboratory of Enteric Infections Россия

A. V. Zabrovskaia

St. Petersburg Pasteur Institute, St. Petersburg

Email: kaflidia@mail.ru
PhD (Veterinary), Senior Researcher, Laboratory of Enteric Infections Россия

S. A. Egorova

St. Petersburg Pasteur Institute, St. Petersburg

Email: kaflidia@mail.ru
PhD (Medicine), Senior Researcher, Laboratory of Enteric Infections Россия

M. A. Makarova

St. Petersburg Pasteur Institute, St. Petersburg

Email: kaflidia@mail.ru
PhD (Medicine), Senior Researcher, Laboratory of Enteric Infections Россия

Z. N. Matveeva

St. Petersburg Pasteur Institute, St. Petersburg

Email: kaflidia@mail.ru
PhD (Medicine), Leading Researcher, Laboratory of Enteric Infections Россия

E. V. Zueva

St. Petersburg Pasteur Institute, St. Petersburg

Email: kaflidia@mail.ru
PhD (Biology), Senior Researcher, Laboratory of Molecular Immunology Россия

A. A. Porin

St. Petersburg Pasteur Institute, St. Petersburg

Email: kaflidia@mail.ru
PhD (Medicine), Researcher, Laboratory of Enteric Infections Россия

M. Y. Boiro

Research Institute for Applied Biology of Guinea, Kindia

Email: kaflidia@mail.ru
PhD (Biology), Professor, General Director of Pasteur Institute of Guinea Гвинея

O. K. Konstantinov

Research Institute for Applied Biology of Guinea, Kindia

Email: kaflidia@mail.ru
PhD (Biology), Researcher, Pasteur Institute of Guinea Гвинея

A. A. Totolian

St. Petersburg Pasteur Institute, St. Petersburg

Email: kaflidia@mail.ru
RAS Full Member, PhD, MD (Medicine), Professor, Head of the Laboratory of Molecular Immunology, Director Россия

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Copyright (c) 2018 Popova A.Y., Kaftyreva L.A., Suzhaeva L.V., Voitenkova E.V., Zabrovskaia A.V., Egorova S.A., Makarova M.A., Matveeva Z.N., Zueva E.V., Porin A.A., Boiro M.Y., Konstantinov O.K., Totolian A.A.

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