Email this article THE STUDY OF ENTEROTOXIGENICITY OF THE BIOTYPE 1A YERSINIA ENTEROCOLITICA
- Authors: Bogumilchik E.A.1, Voskresenskaya E.A.1
-
Affiliations:
- ФГУН «Санкт-Петербургский НИИ эпидемиологии и микробиологии имени Пастера» Роспотребнадзора, Санкт-Петербург
- Issue: Vol 1, No 3 (2011)
- Pages: 263-266
- Section: ORIGINAL ARTICLES
- URL: https://iimmun.ru/iimm/article/view/56
- DOI: https://doi.org/10.15789/2220-7619-2011-3-263-266
- ID: 56
Cite item
Full Text
Abstract
Abstract. The representatives of Y. enterocolitica biotype 1А which are considered as nonpathogenic microorganisms were tested for production of the thermostable enterotoxin YST B (Yersinia Stable Toxin). This toxin is characterized by strong toxic action and it can bring on diarrhea in human and animals. The chromosome gene of thermostable enterotoxin ystB was detected by PCR in 87.1% out of 116 studied strains of different origin and territorial isolation. To determine toxin production in vitro the studied strains cultivated in various conditions: in 26°C and 37°С in usual culture medium and in 37°С in the medium corresponded to the content of intestine. In part of the studied strains the toxin production was revealed on the model of newborn mice in both temperature regimes of cultivation 26°С and 37°С. The study of toxin production in representatives of Y. enterocolitica biotype 1А showed their possible role as etiological agents of diarrhea.
About the authors
E. A. Bogumilchik
ФГУН «Санкт-Петербургский НИИ эпидемиологии и микробиологии имени Пастера» Роспотребнадзора, Санкт-Петербург
Author for correspondence.
Email: tsenevapasteur@yandex.ru
младший научный сотрудник лаборатории бактериальных капельных инфекций
197101, Санкт-Петербург, ул. Мира, 14
Russian FederationE. A. Voskresenskaya
ФГУН «Санкт-Петербургский НИИ эпидемиологии и микробиологии имени Пастера» Роспотребнадзора, Санкт-Петербург
Email: tsenevapasteur@yandex.ru
Russian Federation
References
- Способ определения энтеротоксигенности кишечных палочек на мышах-сосунках: Метод. рекомендации. — Л.: НИИ эпидемиологии и микробиологии им. Пастера, 1980. — 11 с.
- Amirmozafari N., Robertson D.C. Nutritional requirements for synthesis of heat-stable enterotoxin by Yersinia enterocolitica // J. Appl. Envir. Microb. — 1993. — Vol. 59, N 10. — P. 3314–3320.
- Bottone E.J. Yersinia enterocolitica: overview and epidemiologic correlates // J. Microb. Infect. — 1999. — N 1. — P. 323–333.
- Dean A.G., Ching Y.-C., Williams R.G., Harden L.B. Test for Escherichia coli enterotoxin using infant mice: application in study of diarrhea in children in Honolulu // J. Infect. Dis. — 1972. — N 125. — P. 407– 411.
- Delor I., Cornelis G.R. Role of Yersinia enterocolitica Yst toxin in experimental infection of young rabbits // J. Infect. Immun. — 1992. — Vol. 60, N 10. — P. 4269–4277.
- Grant T, Bennett-Wood V., Robins-Browne R. Identification of virulence-associated characteristics in clinical isolates of Yersinia enterocolitica lacking classical virulence markers // J. Infect. Immun. — 1998. — Vol. 66, N 3. — 1113–1120.
- Mikulskis A.V., Delor I., Thi V.H., Cornelis G.R. Regulation of the Yersinia enterocolitica enterotoxin yst gene. Influence of growth phase, temperature, osmolarity, pH and bacterial host factors // J. Mol. Microbiol. — 1994. — N 14. — P. 905–915.
- Pai C.H., Mors V. Production of enterotoxin by Yersinia enterocolitica // J. Infect. Immun. — 1978. — Vol. 19, N 3. — P. 908–911.
- Ramamurthy T., Yoshino K., Huang X., Balakrish Nair G., Carniel E., Maruyama T., Fukushima H., Takeda T. The novel heat-stable enterotoxin subtype gene (ystB) of Yersinia enterocolitica: nucleotide sequence and distribution of the yst genes // J. Microbial. Pathog. — 1997. — N 23. — P. 189–200.
- Singh I., Virdi J.S. Production of Yersinia stable toxin (YST) and distribution of yst genes in biotype 1A strains of Yersinia enterocolitica // J. Med. Microb. — 2004. — N 53. — P. 1065–1068.
- Tennant S.M., Grant T.H., Robins-Browne R.M. Pathogenicity of Yersinia enterocolitica biotype 1A // FEMS Immun. Med. Microb. — 2003. — N 38. — P. 127–137.
- Thoerner P., Bin Kingombe C.I., Bögli-Stuber K., Bissig-Choisat B., Wassenaar T.M., Frey J., Jemmi T. PCR detection of virulence genes in Yersinia enterocolitica and Yersinia pseudotuberculosis and investigation of virulence gene distribution // J. Appl. Env. Microb. — 2003. — Vol. 69, N 3. — P. 1810–1816.
Supplementary files
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).