Email this article THE INFLUENCE OF PATHOGENETIC THERAPY ON THE LEVER OF CYTOKINES IN PATIENTS WITH ACUTE BRUCELLOSIS
- Authors: Kovalevich N.I.1, Sarkisyan N.S.1, Rakitina E.L.1, Galyas V.A.1, Sannikova I.V.2, Makhinya O.V.2
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Affiliations:
- Federal Governmental Health Agency Stavropol anti Plague Institute of Rospotrebnadzor, Stavropol, Russian Federation
- Stavropol State Medical University, Stavropol, Russian Federation
- Issue: Vol 6, No 4 (2016)
- Pages: 384-388
- Section: SHORT COMMUNICATIONS
- Submitted: 06.01.2017
- Accepted: 06.01.2017
- Published: 06.12.2016
- URL: https://iimmun.ru/iimm/article/view/452
- DOI: https://doi.org/10.15789/2220-7619-2016-4-384-388
- ID: 452
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Abstract
The purpose of the study was to determine the level of proinflammatory cytokines: IL-12, IL-8 and IFNγ, neopterin and lipopolysaccharide-binding protein in the serum of patients with acute brucellosis before and after antibiotic therapy. The clinical data from 32 patients with laboratory-confirmed diagnosis — “acute brucellosis” admitted to the diagnosis, treatment and examination of occupational diseases brucellosis GBUZ SC “City Clinical Hospital No. 2”, the city of Stavropol were used in the study. The concentrations IL-12, IL-8, IFNγ cytokines and acute-phase proteins in serum was determined by ELISA. In the acute phase of brucellosis infection (before treatment) had high levels of pro-inflammatory cytokines IL-8 and IFNγ, but despite holding a course of antibiotic treatment in the serum of patients with preserved high levels of IL-8, indicative of active inflammation in the absence of clinical manifestations. IL-12 level, a key cytokine in the initiation of lymphocyte-dependent immune response was lower than in the control group. Evaluation of the cytokine status (IL-8, IL-12, IL-18) and proteins of acute inflammation phase (neopterin and lipopolysaccharide-binding protein) will provide valuable information for monitoring the effect of pharmacotherapy of acute brucellosis. Indicators of lipopolysaccharide-binding protein and neopterin in the serum of patients with brucellosis should be considered as a marker of inflammatory activity and as a predictor of outcome of acute brucellosis.
About the authors
N. I. Kovalevich
Federal Governmental Health Agency Stavropol anti Plague Institute of Rospotrebnadzor, Stavropol, Russian Federation
Author for correspondence.
Email: nyshik@yandex.ru
PhD (Medicine), Head of the Research and Prevention Clinical Diagnostic Laboratory Россия
N. S. Sarkisyan
Federal Governmental Health Agency Stavropol anti Plague Institute of Rospotrebnadzor, Stavropol, Russian Federation
Email: nyshik@yandex.ru
Physician of Clinical Laboratory Diagnostics, Research and Prevention Clinical Diagnostic Laboratory Россия
E. L. Rakitina
Federal Governmental Health Agency Stavropol anti Plague Institute of Rospotrebnadzor, Stavropol, Russian Federation
Email: nyshik@yandex.ru
PhD (Medicine), Leading Researcher, Laboratory of Bruzellosis, Sector of Immunology and Pathomorphology of Especially Dangerous Infections Россия
V. A. Galyas
Federal Governmental Health Agency Stavropol anti Plague Institute of Rospotrebnadzor, Stavropol, Russian Federation
Email: nyshik@yandex.ru
Physician of Clinical Laboratory Diagnostics, Research and Prevention Clinical Diagnostic Laboratory Россия
I. V. Sannikova
Stavropol State Medical University, Stavropol, Russian Federation
Email: nyshik@yandex.ru
PhD, MD (Medicine), Professor of the Department of Infectious Diseases and Phthisiatry Россия
O. V. Makhinya
Stavropol State Medical University, Stavropol, Russian Federation
Email: nyshik@yandex.ru
PhD Candidate, Department of Infectious Diseases and Phthisiatry, Россия
References
- Жумадилова З.К., Байсугуров Ж.Б. Ферментные системы фагоцитирующих мононуклеаров при различных вариантах течения бруцеллеза // Здравоохранение Казахстана. 1989. № 6. С. 29–31. [Zhumadilova Z.K., Baysugurov J.B. Mononuclear phagocyte enzyme system in different variants of flow of brucellosis. Zdravookhranenie Kazakhstana = Healthcare in Kazakhstan, 1989, no. 6, pp. 29–31 (In Russ.)]
- Инфекционные болезни: национальное руководство / под ред. Н.Д. Ющука, Ю.Я. Венгерова. М.: ГЭОТАР-Медиа, 2009. 1040 с. [Infektsionnye bolezni: natsional’noe rukovodstvo / pod red. N.D. Yushchuka, Yu.Ya. Vengerova [Infectious diseases: national guideline. Eds. N.D. Yushchuk, U.Yu. Vengerov]. Moscow: GEOTAR-Media, 2009. 1040 p.
- Лямкин Г.И., Пономаренко Д.Г., Худолеев А.А., Вилинская С.В., Зайцев А.А., Куличенко А.Н. Эпидемическая ситуация по бруцеллезу в Российской Федерации и государствах — участниках Содружества Независимых Государств // Инфекционные болезни: новости, мнения, обучение. 2016. № 1. С. 68–74. [Lyamkin G.I., Ponomarenko D.G., Khudoleev A.A., Vilinskay S.V., Zaitsev A.A., Kulichenko A.N. Epidemiological situation on brucellosis in the Russian Federation and the states — participants of the Commonwealth of Independent States. Infektsionnye bolezni: novosti, mneniya, obuchenie = Infectious Diseases: News, Opinions, Training, 2016, no. 1, pp. 68–74. (In Russ.)]
- Свиридов Е.А., Телегина Т.А. Неоптерин и его восстановленные формы: биологическая роль и участие в клеточном иммунитете // Успехи биологической химии. 2005. Т. 45. С. 355–390. [Sviridov Е .A., Telegina T.A. Neopterin and its reduced forms: biological role and participation in cellular immunity. Uspekhi biologicheskoy khimii = Advances in Biochemistry, 2005, vol. 45, pp. 355–390. (In Russ.)]
- Ariza J., Corredoira J., Pallares R., Viladrich P.F., Rufi G., Pujol M., Gudiol F. Characteristics of and risk factors for relapse of brucellosis in humans. Clin. Infect. Dis., 1995, vol. 20, pp. 1241–1249. doi: 10.1093/clinids/20.5.1241
- Boschiroli M.L. Foulongne V., Callaghan D.O. Brucellosis: a worldwide zoonosis. Curr. Opin. Microbiol., 2001, vol. 4, pp. 58–64. doi: 10.1016/S1369-5274(00)00165-X
- Liautard J.P., Gross A., Dornand J., Köhler S. Interactions between professional phagocytes and Brucella spp. Microbiologia, 1996, vol. 12 (2), pp. 197–206.
- Mantur B.G. Amarnath S.K., Shinde R.S. Review of clinical and laboratory features of human brucellosis. J. Indian Med. Microbiol., 2007, vol. 25 (3), pp. 188–202. doi: 10.4103/0255-0857.34758
- Ocon P., Reguera J.M., Morata P., Juarez C., Alonso A., Colmenero J.D. Phagocytic cell function in active brucellosis. Infect. Immun., 1994, vol. 62, pp. 910–914.
- Skendros P., Pappas G., Boura P. Cell-mediated immunity in human brucellosis. Microbes Infect., 2011, vol. 13 (2), pp. 134–142. doi: 10.1016/j.micinf.2010.10.015
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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).