THE CHARACTERIZATION OF PROAPOPTOTIC ACTIVITY OF LIPOPOLYSACCHARIDE IN ISOLATED SUBPOPULATIONS OF HUMAN T-LYMPHOCYTES

Cover Page
  • Authors: Filatova E.N.1, Anisenkova E.V.2, Presnyakova N.B.2, Neumoina N.V.2, Sycheva T.D.3, Utkin O.V.3
  • Affiliations:
    1. Blokhina Research Institute of Epidemiology and Microbiology of Federal Service on Surveillance for Consumer Rights Protection and Human Welfare, Nizhny Novgorod, Russian Federation 603950, Russian Federation, Nizhni Novgorod, Malaya Yamskaya str., 71, Nizhny Novgorod State Medical Academy. Phone: +7 (831) 469-79-46, +7 (906) 368-37-22. Fax: +7 (831) 469-79-20.
    2. Blokhina Research Institute of Epidemiology and Microbiology of Federal Service on Surveillance for Consumer Rights Protection and Human Welfare, Nizhny Novgorod, Russian Federation
    3. Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russian Federation
  • Issue: Vol 4, No 4 (2014)
  • Pages: 347-352
  • Section: ORIGINAL ARTICLES
  • Submitted: 03.02.2015
  • Accepted: 03.02.2015
  • Published: 03.02.2015
  • URL: https://iimmun.ru/iimm/article/view/245
  • DOI: https://doi.org/10.15789/2220-7619-2014-4-347-352
  • ID: 245


Cite item

Full Text

Abstract

Lipopolysaccharide (LPS) is used as activator and inductor of immune cells apoptosis in case of infectious processes modeling. It is not clear if LPS is direct inductor of apoptosis in human T-cells. The subpopulations of effector and naive CD4+ T-helper and CD8+ cytotoxic T lymphocytes of human peripheric blood were isolated and cultivated in presence of LPS. It has been shown that LPS was inductor of apoptosis in studying T-cells populations.

About the authors

E. N. Filatova

Blokhina Research Institute of Epidemiology and Microbiology of Federal Service on Surveillance for Consumer Rights Protection and Human Welfare, Nizhny Novgorod, Russian Federation 603950, Russian Federation, Nizhni Novgorod, Malaya Yamskaya str., 71, Nizhny Novgorod State Medical Academy. Phone: +7 (831) 469-79-46, +7 (906) 368-37-22. Fax: +7 (831) 469-79-20.

Author for correspondence.
Email: el.filatova83@gmail.com

PhD (Biology), Leading Researcher, Laboratory of Molecular Biology and Biotechnology of Blokhina Research Institute of Epidemiology and Microbiology of Federal Service on Surveillance for Consumer Rights Protection and Human Welfare, Nizhny Novgorod, Russian Federation

Россия

E. V. Anisenkova

Blokhina Research Institute of Epidemiology and Microbiology of Federal Service on Surveillance for Consumer Rights Protection
and Human Welfare, Nizhny Novgorod, Russian Federation

Email: fake@neicon.ru

Junior Researcher Laboratory of Molecular Biology and Biotechnology of Blokhina Research Institute of Epidemiology and Microbiology of Federal Service on Surveillance for Consumer Rights Protection and Human Welfare, Nizhny Novgorod, Russian Federation

Россия

N. B. Presnyakova

Blokhina Research Institute of Epidemiology and Microbiology of Federal Service on Surveillance for Consumer Rights Protection
and Human Welfare, Nizhny Novgorod, Russian Federation

Email: fake@neicon.ru

Researcher Scientist Laboratory of Molecular Biology and Biotechnology of Blokhina Research Institute of Epidemiology and Microbiology of Federal Service on Surveillance for Consumer Rights Protection and Human Welfare, Nizhny Novgorod, Russian Federation

Россия

N. V. Neumoina

Blokhina Research Institute of Epidemiology and Microbiology of Federal Service on Surveillance for Consumer Rights Protection
and Human Welfare, Nizhny Novgorod, Russian Federation

Email: fake@neicon.ru

PhD (Medicine), Medical Director, Clinic of Inflectious Deseases of Blokhina Research Institute of Epidemiology and Microbiology of Federal Service on Surveillance for Consumer Rights Protection and Human Welfare, Nizhny Novgorod, Russian Federation

Россия

T. D. Sycheva

Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russian Federation

Email: fake@neicon.ru

Resident Physician, Department of Children Deseases of Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russian Federation

Россия

O. V. Utkin

Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russian Federation

Email: fake@neicon.ru

PhD (Biology), Head of Laboratory of Molecular Biology
and Biotechnology of Blokhina Research Institute of Epidemiology and Microbiology of Federal Service on Surveillance for Consumer Rights Protection and Human Welfare, Assistant Professor of the Department of Microbiology and Immunology of Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russian Federation

Россия

References

  1. Akashi S., Shimazu R., Ogata H., Nagai Y., Takeda K., Kimoto M., Miyake K. Cutting edge: cell surface e 1. xpression and lipopolysaccharide signaling via the Toll-like receptor 4-MD-2 complex on mouse peritoneal macrophages. J. Immunol., 2000, vol. 164, no. 7, pp. 3471–3475.
  2. Beutler B. TLR4: central component of the sole mammalian LPS sensor. Curr. Opin. Immunol., 2000, vol. 12, no. 3, pp. 20–26.
  3. Caramalho I., Lopes-Carvalho T., Ostler D., Zelenay S., Haury M., Demengeot J. Regulatory T cells selectively express Toll-like receptors and are activated by lipopolysaccharide. J. Exp. Med., 2003, vol. 197, no. 4, pp. 403–411.
  4. Crellin N.K., Garcia R.V., Hadisfar O., Allan S.E., Steiner T.S., Levings M.K. Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells. J. Immunol., 2005, vol. 175, no. 12, pp. 8051–8059.
  5. Da Silva C.J., Soldau K., Christen U., Tobias P.S., Ulevitch R.J. Lipopolysaccharide is in close proximity to each of the proteins in its membrane receptor complex transfer from CD14 to TLR4 and MD-2. J. Biol. Chem., 2001, vol. 276, no. 24, pp. 21129–21135.
  6. Gelman A.E., Zhang J., Choi Y., Turka L.A. Toll-like receptor ligands directly promote activated CD4+T cell survival. J. Immunol., 2004, vol. 172, no. 10, pp. 6065–6073.
  7. Gonzalez-Navaias J.M., Fine S., Law J., Data S.K., Nguyen K.P., Yu M., Corr M., Katakura K., Eckman L., Lee J., Raz E. TLR4 signaling in effector CD4+ T cells regulates TCR activation and experimental colitis in mice. J. Clin. Invest., 2010, vol. 120, no. 2, pp. 570–581.
  8. Gupta S., Gollapudi S. Susceptibility of naive and subsets of memory T cells to apoptosis via multiple signaling pathways. Autoimmune Rev., 2007, vol. 6, no. 7, pp. 476–481.
  9. Hornung V., Rothenfusser S., Britsch S., Krug A., Jahrsdorfer B., Giese T., Endres S., Hartman G. Quantitative expression of tolllike receptor 1–10 mRNA in cellular subsets of human peripheral blood mononuclear cells and sensitivity to CpG oligodeoxynucleotides. J. Immunol., 2002, vol. 168, no. 9, pp. 4531–4537.
  10. Kaisho T., Akira S. Dendritic-cell function in toll-like receptor- and MyD88-knockout mice. Trends Immunol., 2001, vol. 22, no. 2, pp. 78–83.
  11. Komai-Koma M., Jones L., Ogg G.S., Xu D., Liew F.Y. TLR2 is expressed on activated T cells as a costimulatory receptor. Proc. Natl. Acad. Sci. USA, 2004, vol. 101, no. 9, pp. 3029–3034.
  12. Komai-Koma M., Gilchrist D.S., Xu D. Direct recognition of LPS by human but not murine CD8+ T cells via TLR4 complex. Eur. J. Immunol., 2009, vol. 39, no. 6, pp. 1564–1572.
  13. Liew F.Y., Xu D., Brint E.K., O’Neill L.A. Negative regulation of toll-like receptor-mediated immune responses. Nat. Rev. Immunol., 2005, vol. 5, pp. 446–458.
  14. Mburu S., Marnewick J.L., Abayomi A., Ipp H. Modulation of LPS-induced CD4+ T-cell activation and apoptosis by antioxidants in untreated asymptomatic HIV infected participants: an in vitro study. Clin. Dev. Immunol., 2013, vol. 2013, 9 p. Available at: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3856122.
  15. Medzhitov R., Preston-Hurlburt P., Janeway С.А. Jr. A human homologue of the Drosophila Toll protein signals activation of adaptive immunity. Nature, 1997, vol. 388, no. 6640, pp. 394–397.
  16. Muzio M., Polentarutti N., Bosisio D., Prahladan M.K., Mantovani A. Toll-like receptor family (TLT) and signaling pathway. Eur. Cytokine Netw., 2000, vol. 11, no. 3, pp. 489–490.
  17. Nielsen J.S., Larsson A., Brix-Christensen V., Nvengaard J.R., Ledet T., Tonnesen E. Endotoxemia-induced lymphocyte apoptosis is augmented by a hyperinsulinemic-euglycemic clamp. J. Anesthesiology, 2005, vol. 102, no. 4, pp. 768–773.
  18. Nielsen J.S., Larsson A., Ledet T., Turina M., Tonnesen E., Krog J. Rough-form lipopolysaccharide increases apoptosis in human CD4+ and CD8+ T lymphocytes. Scand. J. Immunol., 2011, vol. 75, pp. 193–202.
  19. Perl M., Chung C.S., Swan R., Ayala A. Role of programmed cell death in the immunopathogenesis of sepsis. Drug. Discov. Today Dis. Mech., 2007, vol. 4, no. 4, pp. 223–320.
  20. Raetz C.R. Biochemistry of endotoxins. Annu. Rev. Biochem., 1990, vol. 59, pp. 129–170.
  21. Schromm A.B., Lien E., Henneke P., Chow J.C., Yoshimura A., Heine H., Latz E., Monks B.G., Schwartz D.A., Miyake K., Golenbock D.T. Molecular genetic analysis of an endotoxin nonresponder mutant cell line: a point mutation in a conserved region of MD-2 abolishes endotoxin-induced signaling. J. Exp. Med., 2001, vol. 194, no. 1, pp. 79–88.
  22. Sutmuller R.P., den Brok M.H., Kramer M., Bennink E.J., Toonen L.W., Kullberg B.J., Joosten L.A., Akira S., Netea M.G., Adema G.J. Toll-like receptor 2 controls expansion and function of regulatory T cells. J. Clin. Invest., 2006, vol. 116, no. 2, pp. 485–494.
  23. Takeda K., Kaisho T., Akira S. Toll-like receptors. Annu. Rev. Immunol., 2003, vol. 21, pp. 335–376.
  24. Tian J., Zekzer D., Hanssen L., lu Y., Olcott A., Kaufman D.L. Lipopolysaccharide-activated B cells down-regulate Th1 immunity and prevent autoimmune diabetes in nonobese diabetic mice. J. Immunol., 2001, vol. 167, no. 2, pp. 1081–1089.
  25. Ulevitch R.J., Tobias P.S. Receptor-dependent mechanisms of cell stimulation by bacterial endotoxin. Annu. Rev. Immunol., 1995, vol. 13, pp. 437–457.
  26. Zarember K.A., Godowski P.J. Tissue expression of human Toll-like receptors and differential regulation of Toll-like receptor mRNAs in leukocytes in response to microbes, their products, and cytokines. J. Immunol., 2002, vol. 168, no. 2, pp. 554–561.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2015 Filatova E.N., Anisenkova E.V., Presnyakova N.B., Neumoina N.V., Sycheva T.D., Utkin O.V.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 64788 от 02.02.2016.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies