Cellular and humoral immune parameters in the peripheral and local blood flow in patients with primary pelvic varicose

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Abstract

Pelviс varicose veins (PVV) in women is of extremely high relevance due to a close relationship with reproductive disorders and disease relapse. Despite on research in the field, the results of the analysis on immune reactivity in PVV are rather contradictory, and mainly relate to general mechanisms. The study of cell-mediated and humoral arms of adaptive immunity will allow us to assess an intensity and dynamics of PVV progression as well as a potential for using immune status parameters to optimize diagnosis and immunological correction. The aim of this study was to assess characteristics of cellular and humoral immunity in peripheral (ulnar) and local (ovarian) venous pools in women with mild and moderate PVV. The study involved 142 women of reproductive age (mean age — 37.2±7.1 years) with diagnosed PVV — mild (Group 1) (n = 79) and moderate (Group 2) (n = 63) forms. Data from 30 apparently healthy women (mean age 33.5±6.3 years) were in control group. Flow cytometry was used to identify lymphocyte subsets. The functional state of the humoral immunity was assessed by measuring concentration of immunoglobulins IgA, IgG, IgM by using radial immunodiffusion in the gel. According to our data obtained, patients in group 1 had changes found only in the local blood flow such as prominent lymphocytopenia (decreased level of CD3+ and CD4+ lymphocytes). Patients in group 2 were featured with more pronounced changes: increased lymphocyte level, CD3+, CD4+/CD8+, IgA and lower levels of IgM, IgG in peripheral blood; high average leukocyte and lymphocyte count, IgA and reduced levels of CD3+, CD4+, CD4+/CD8+, IgM and IgG in the local area. It can be concluded that altered parameters in the local blood flow are of primary origin in mild PVV being characterized by disturbed cellular immune arm. Along with increasing disease severity, the host compensatory capabilities decline, which is manifested by pronounced combined immunodeficiency at the level of local blood flow less evident at peripheral level. These results may contribute to a more accurate assessment of intensity and dynamics of PVV progression to optimize diagnostics and immunological correction.

About the authors

Marina A. Darenskaya

Scientific Centre of the Family Health and Human Reproduction Problems

Author for correspondence.
Email: marina_darenskaya@inbox.ru

DSc (Biology), Professor of the RAS, Leading Researcher, Laboratory of Pathophysiology

Russian Federation, Irkutsk

Andrey A. Semendyaev

Irkutsk State Medical University

Email: marina_darenskaya@inbox.ru

DSc (Medicine), Professor of the Department of Obstetrics and Gynecology with a Course of Gynecology for Children and Adolescents

Russian Federation, Irkutsk

Dmitry A. Stupin

Scientific Centre of the Family Health and Human Reproduction Problems; Irkutsk State Medical University

Email: marina_darenskaya@inbox.ru

PhD (Medicine), Doctoral Candidate; Assistant Professor, Department of Obstetrics and Gynecology with a Course of Gynecology for Children and Adolescents

Russian Federation, Irkutsk; Irkutsk

Sergey I. Kolesnikov

Scientific Centre of the Family Health and Human Reproduction Problems

Email: marina_darenskaya@inbox.ru

RAS Full Member, DSc (Medicine), Professor, Head Researcher

Russian Federation, Irkutsk

Natalya V. Semenova

Scientific Centre of the Family Health and Human Reproduction Problems

Email: marina_darenskaya@inbox.ru

DSc (Biology), Leading Researcher, Laboratory of Pathophysiology

Russian Federation, Irkutsk

Konstantin V. Pesterev

Irkutsk State Medical University

Email: marina_darenskaya@inbox.ru

Postgraduate Student, Department of Obstetrics and Gynecology with a course of Gynecology for Children and Adolescents

Russian Federation, Irkutsk

Lyubov I. Kolesnikova

Scientific Centre of the Family Health and Human Reproduction Problems

Email: marina_darenskaya@inbox.ru

RAS Full Member, DSc (Medicine), Professor, Scientific Director

Russian Federation, Irkutsk

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Copyright (c) Darenskaya M.A., Semendyaev A.A., Stupin D.A., Kolesnikov S.I., Semenova N.V., Pesterev K.V., Kolesnikova L.I.

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