Cellular and humoral immune parameters in the peripheral and local blood flow in patients with primary pelvic varicose

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Abstract

Pelviс varicose veins (PVV) in women is of extremely high relevance due to a close relationship with reproductive disorders and disease relapse. Despite on research in the field, the results of the analysis on immune reactivity in PVV are rather contradictory, and mainly relate to general mechanisms. The study of cell-mediated and humoral arms of adaptive immunity will allow us to assess an intensity and dynamics of PVV progression as well as a potential for using immune status parameters to optimize diagnosis and immunological correction. The aim of this study was to assess characteristics of cellular and humoral immunity in peripheral (ulnar) and local (ovarian) venous pools in women with mild and moderate PVV. The study involved 142 women of reproductive age (mean age — 37.2±7.1 years) with diagnosed PVV — mild (Group 1) (n = 79) and moderate (Group 2) (n = 63) forms. Data from 30 apparently healthy women (mean age 33.5±6.3 years) were in control group. Flow cytometry was used to identify lymphocyte subsets. The functional state of the humoral immunity was assessed by measuring concentration of immunoglobulins IgA, IgG, IgM by using radial immunodiffusion in the gel. According to our data obtained, patients in group 1 had changes found only in the local blood flow such as prominent lymphocytopenia (decreased level of CD3+ and CD4+ lymphocytes). Patients in group 2 were featured with more pronounced changes: increased lymphocyte level, CD3+, CD4+/CD8+, IgA and lower levels of IgM, IgG in peripheral blood; high average leukocyte and lymphocyte count, IgA and reduced levels of CD3+, CD4+, CD4+/CD8+, IgM and IgG in the local area. It can be concluded that altered parameters in the local blood flow are of primary origin in mild PVV being characterized by disturbed cellular immune arm. Along with increasing disease severity, the host compensatory capabilities decline, which is manifested by pronounced combined immunodeficiency at the level of local blood flow less evident at peripheral level. These results may contribute to a more accurate assessment of intensity and dynamics of PVV progression to optimize diagnostics and immunological correction.

About the authors

Marina A. Darenskaya

Scientific Centre of the Family Health and Human Reproduction Problems

Author for correspondence.
Email: marina_darenskaya@inbox.ru

DSc (Biology), Professor of the RAS, Leading Researcher, Laboratory of Pathophysiology

Russian Federation, Irkutsk

Andrey A. Semendyaev

Irkutsk State Medical University

Email: marina_darenskaya@inbox.ru

DSc (Medicine), Professor of the Department of Obstetrics and Gynecology with a Course of Gynecology for Children and Adolescents

Russian Federation, Irkutsk

Dmitry A. Stupin

Scientific Centre of the Family Health and Human Reproduction Problems; Irkutsk State Medical University

Email: marina_darenskaya@inbox.ru

PhD (Medicine), Doctoral Candidate; Assistant Professor, Department of Obstetrics and Gynecology with a Course of Gynecology for Children and Adolescents

Russian Federation, Irkutsk; Irkutsk

Sergey I. Kolesnikov

Scientific Centre of the Family Health and Human Reproduction Problems

Email: marina_darenskaya@inbox.ru

RAS Full Member, DSc (Medicine), Professor, Head Researcher

Russian Federation, Irkutsk

Natalya V. Semenova

Scientific Centre of the Family Health and Human Reproduction Problems

Email: marina_darenskaya@inbox.ru

DSc (Biology), Leading Researcher, Laboratory of Pathophysiology

Russian Federation, Irkutsk

Konstantin V. Pesterev

Irkutsk State Medical University

Email: marina_darenskaya@inbox.ru

Postgraduate Student, Department of Obstetrics and Gynecology with a course of Gynecology for Children and Adolescents

Russian Federation, Irkutsk

Lyubov I. Kolesnikova

Scientific Centre of the Family Health and Human Reproduction Problems

Email: marina_darenskaya@inbox.ru

RAS Full Member, DSc (Medicine), Professor, Scientific Director

Russian Federation, Irkutsk

References

  1. Головина В.И., Селиверстов Е.И., Ефремова О.И., Золотухин И.А. Роль цитокинов в патогенезе варикозной болезни // Флебология. 2021. Т. 15, № 2. C. 117–126. [Golovina V.I., Seliverstov E.I., Efremova O.I., Zolotukhin I.A. Cytokines in pathogenesis of varicose veins. Flebologiya = Flebologiya, 2021,vol. 15, no. 2, pp. 117–126. (In Russ.)] doi: 10.17116/flebo202115021117
  2. Даренская М.А., Ступин Д.А., Семендяев А.А., Колесников С.И., Гребенкина Л.А., Щербатых А.В., Толкачев К.С., Колесникова Л.И. Сравнительный анализ липопероксидации в периферическом и регионарном кровотоках у женщин с первичным варикозным расширением вен малого таза // Клиническая лабораторная диагностика. 2022. Т. 67, № 7. С. 374–380. [Darenskaya M.A., Stupin D.A., Semendyaev A.A., Kolesnikov S.I., Grebenkina L.A., Shcherbatykh A.V., Tolkachev K.S., Kolesnikova L.I. Peripheral and regional blood bloodstreams lipid peroxidation comparative analysis in women with pelvic venous disorder. Klinicheskaya laboratornaya diagnostika = Russian Clinical Laboratory Diagnostics, 2022, vol. 67, no. 7, pp. 374–380. (In Russ.)] doi: 10.51620/0869-2084-2022-67-7-374-380
  3. Зинатулина Г.М., Фазылов В.Х., Василькин Д.А. Особенности иммунного статуса у больных микробной экземой и рожей: метод терапевтической коррекции // Вестник дерматологии и венерологии. 2016. № 2. С. 87–94. [Zinatulina G.M., Fayzullina E.V., Fazyilov V.H., Vasilkin D.A. Particular features of the immune status in microbial eczema and erysipelas patients: a therapeutic method. Vestnik dermatologii i venerologii = Vestnik Dermatologii i Venerologii, 2016, no. 2, pp. 87–95. (In Russ.)]
  4. Карзакова Л.М., Кудряшов С.И., Луткова Т.С., Соколова Е.В., Сидоров И.А. Основы общей иммунологии: учеб. пособие. Чебоксары: Издательство Чувашского университета, 2020. 200 с. [Karzakova L.M., Kudryashov S.I., Lutkova T.S., Sokolova E.V., Sidorov I.A. Fundamentals of general immunology: tutorial. Cheboksary: Chuvash University Publishing, 2020. 200 p. (In Russ.)]
  5. Колесникова Л.И., Семендяев А.А., Ступин Д.А., Даренская М.А., Гребёнкина Л.А., Натяганова Л.В., Данусевич И.Н., Черепанова М.А., Колесников С.И. Интенсивность процессов липопероксидации у женщин с первичным варикозным расширением вен малого таза в зависимости от стадии заболевания // Вестник Российской академии медицинских наук. 2018. Т. 73, № 4. С. 229–235. [Kolesnikova L.I., Semendyaev A.A., Stupin D.A., Darenskaya M.A., Grebenkina L.A., Natyaganova L.V., Danusevich I.N., Cherepanova M.A., Kolesnikov S.I. The intensity of lipid peroxidation processes in women with primary varicose veins of the pelvic depending on the stage of the disease. Vestnik Rossiiskoi akademii meditsinskikh nauk = Annals of the Russian Academy of Medical Sciences, 2018, vol. 73, no. 4, pp. 229–235. (In Russ.)] doi: 10.15690/vramn1005
  6. Колесникова Л.И., Гус А.И., Семендяев А.А., Ступин Д.А., Даренская М.А., Колесников С.И., Гребенкина Л.А., Щербатых А.В., Калягин А.Н., Пурбуева В.Н. Варикозная болезнь вен малого таза у женщин. Новосибирск: Наука, 2020. 156 c. [Kolesnikova L.I., Gus A.I., Semendyaev A.A., Stupin D.A., Darenskaya M.A., Kolesnikov S.I., Grebenkina L.A., Shcherbatykh A.V., Kalyagin A.N., Purbueva V.N. Pelvic varicosis in womеn. Novosibirsk: Nauka, 2020. 156 p. (In Russ.)]
  7. Патент № 2646563 Российская Федерация, МПК А61В 10/00 (2006.01). Cпособ определения функционального состояния венозной системы малого таза у женщин: № 2016124576; заявлено 2016.06.20: опубликовано 2018.05.03 / Семендяев А.А., Ступин Д.А., Черепанова М.А., Бачурина В.М., Селецкий А.Н., Колесникова Л.И. Патентообладатель: Семендяев Андрей Александрович. 5 с. [Patent No. 2646563 Russian Federation, Int. Cl. А61В 10/00 (2006.01). Method for determining the functional state of the venous system of the small pelvis in women. No. 2016124576; application: 2016.06.20: date of publication 2018.05.03 / Semendyaev A.A., Stupin D.A., Cherepanova M.A., Bachurina V.M., Seletsky A.N., Kolesnikova L.I. Proprietors: Semendyaev Andrei Aleksandrovich. 5 p.]
  8. Тотолян А.А. Современные подходы к диагностике иммунопатологических состояний // Медицинская иммунология. 1999. Т. 1, № 1–2. С. 75–108. [Totolian A.A. Modern approaches to the diagnosis of immunopathological conditions. Meditsinskaya immunologiya = Medical Immunology (Russia), 1999, vol. 1, no. 1–2, pp. 75–108. (In Russ.)]
  9. Фомин В.С., Фомина М.Н. Варикозное расширение вен малого таза как причина синдрома хронических тазовых болей: взгляд на проблему // Фарматека. 2017. Т. 18, № 351. С. 14–19. [Fomin V.S., Fomina M.N. Varicose veins of the small pelvis as a cause of chronic pelvic pain syndrome: a look at the problem. Farmateka = Farmateka, 2017, vol. 18, no. 351, pp. 14–19. (In Russ.)]
  10. Bałabuszek K., Toborek M., Pietura R. Comprehensive overview of the venous disorder known as pelvic congestion syndrome. Ann. Med., 2022, vol. 54, no. 1, pp. 22–36. doi: 10.1080/07853890.2021.2014556
  11. Barge T.F., Uberoi R. Symptomatic pelvic venous insufficiency: a review of the current controversies in pathophysiology, diagnosis, and management. Clin. Radiol., 2022, vol. 77, no. 6, pp. 409–417. doi: 10.1016/j.crad.2022.01.053
  12. Darenskaya M.A., Stupin D.A., Semendyaev A.A., Kolesnikov S.I., Shcherbatykh A.V., Tolkachev K.S., Kolesnikova L.I. Pelvic venous insufficiency: lipid peroxidation levels in ovarian venous blood. Biomed. Res. Ther., 2022, vol. 9, no. 2, pp. 4884–4891. doi: 10.15419/bmrat.v9i2.730
  13. Gavrilov S.G., Vassilieva G.Y., Vasilev I.M., Grishenkova A.S. The role of vasoactive neuropeptides in the genesis of venous pelvic pain: a review. Phlebology, 2020, vol. 35, no. 1, pp. 4–9. doi: 10.1177/0268355519855598
  14. Khilnani N.M., Winokur R.S., Scherer K.L., Meissner M.H. Clinical presentation and evaluation of pelvic venous disorders in women. Tech. Vasc. Interv. Radiol., 2021, vol. 24, no. 1: 100730. doi: 10.1016/j.tvir.2021.100730
  15. Mancini G., Carbonara A.O., Heremans J.F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry, 1965, no. 2, pp. 235–254. doi: 10.1016/0019-2791(65)90004-2
  16. Meissner M.H., Gloviczki P., Bergan J., Kistner R.L., Morrison N., Pannier F., Pappas P.J., Rabe E., Raju S., Villavicencio J.L. Primary chronic venous disorders. J. Vasc. Surg., 2007, vol. 46, suppl. S, pp. 54S–67S. doi: 10.1016/j.jvs.2007.08.038
  17. Riding D.M., Hansrani V., McCollum C. Pelvic vein insufficiency: clinical perspectives. Vasc. Health Risk Manag., 2017, vol. 13: 439. doi: 10.2147/VHRM.S132827
  18. Sayer G.L., Smith P.D.C. Immunocytochemical characterisation of the inflammatory cell infiltrate of varicose veins. Eur. J. Vasc. Endovasc. Surg., 2004, vol. 5, no. 28, pp. 479–483. doi: 10.1016/j.ejvs.2004.07.023
  19. Seryapina Yu.V., Sevost’yanova K.S., Tulupov A.A., Morozov V.V., Shevela A.I. The genetic predictors of varicose veins of small pelvis: a pilot study. Flebologiya, 2018, vol. 12, no. 1, pp. 25–29. doi: 10.17116/flebo201812125-29
  20. Spath P., Tisato V., Gianesini S., Tessari M., Menegatti E., Manfredini R., Occhionorelli S., Secchiero P., Zamboni P. The calendar of cytokines: seasonal variation of circulating cytokines in chronic venous insufficiency. JRSM Cardiovasc. Dis., 2017, no. 6: 2048004017729279. doi: 10.1177/2048004017729

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Copyright (c) Darenskaya M.A., Semendyaev A.A., Stupin D.A., Kolesnikov S.I., Semenova N.V., Pesterev K.V., Kolesnikova L.I.

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