Russian Journal of Infection and ImmunityRussian Journal of Infection and Immunity2220-76192313-7398SPb RAACI210810.15789/2220-7619-CAH-2108Research ArticleCellular and humoral immune parameters in the peripheral and local blood flow in patients with primary pelvic varicoseDarenskayaMarina A.<p>DSc (Biology), Professor of the RAS, Leading Researcher, Laboratory of Pathophysiology</p>marina_darenskaya@inbox.ruSemendyaevAndrey A.<p>DSc (Medicine), Professor of the Department of Obstetrics and Gynecology with a Course of Gynecology for Children and Adolescents</p>marina_darenskaya@inbox.ruStupinDmitry A.<p>PhD (Medicine), Doctoral Candidate; Assistant Professor, Department of Obstetrics and Gynecology with a Course of Gynecology for Children and Adolescents</p>marina_darenskaya@inbox.ruKolesnikovSergey I.<p>RAS Full Member, DSc (Medicine), Professor, Head Researcher</p>marina_darenskaya@inbox.ruSemenovaNatalya V.<p>DSc (Biology), Leading Researcher, Laboratory of Pathophysiology</p>marina_darenskaya@inbox.ruPesterevKonstantin V.<p>Postgraduate Student, Department of Obstetrics and Gynecology with a course of Gynecology for Children and Adolescents</p>marina_darenskaya@inbox.ruKolesnikovaLyubov I.<p>RAS Full Member, DSc (Medicine), Professor, Scientific Director</p>marina_darenskaya@inbox.ruScientific Centre of the Family Health and Human Reproduction ProblemsIrkutsk State Medical University240420231323553623012202218022023Copyright © 2023, Darenskaya M.A., Semendyaev A.A., Stupin D.A., Kolesnikov S.I., Semenova N.V., Pesterev K.V., Kolesnikova L.I.2023<p>Pelviс varicose veins (PVV) in women is of extremely high relevance due to a close relationship with reproductive disorders and disease relapse. Despite on research in the field, the results of the analysis on immune reactivity in PVV are rather contradictory, and mainly relate to general mechanisms. The study of cell-mediated and humoral arms of adaptive immunity will allow us to assess an intensity and dynamics of PVV progression as well as a potential for using immune status parameters to optimize diagnosis and immunological correction. The aim of this study was to assess characteristics of cellular and humoral immunity in peripheral (ulnar) and local (ovarian) venous pools in women with mild and moderate PVV. The study involved 142 women of reproductive age (mean age 37.27.1 years) with diagnosed PVV mild (Group 1) (n = 79) and moderate (Group 2) (n = 63) forms. Data from 30 apparently healthy women (mean age 33.56.3 years) were in control group. Flow cytometry was used to identify lymphocyte subsets. The functional state of the humoral immunity was assessed by measuring concentration of immunoglobulins IgA, IgG, IgM by using radial immunodiffusion in the gel. According to our data obtained, patients in group 1 had changes found only in the local blood flow such as prominent lymphocytopenia (decreased level of CD3<sup>+</sup> and CD4<sup>+</sup> lymphocytes). Patients in group 2 were featured with more pronounced changes: increased lymphocyte level, CD3<sup>+</sup>, CD4<sup>+</sup>/CD8<sup>+</sup>, IgA and lower levels of IgM, IgG in peripheral blood; high average leukocyte and lymphocyte count, IgA and reduced levels of CD3<sup>+</sup>, CD4<sup>+</sup>, CD4<sup>+</sup>/CD8<sup>+</sup>, IgM and IgG in the local area. It can be concluded that altered parameters in the local blood flow are of primary origin in mild PVV being characterized by disturbed cellular immune arm. Along with increasing disease severity, the host compensatory capabilities decline, which is manifested by pronounced combined immunodeficiency at the level of local blood flow less evident at peripheral level. These results may contribute to a more accurate assessment of intensity and dynamics of PVV progression to optimize diagnostics and immunological correction.</p>varicose veins of the small pelviswomenimmunityimmunoglobulinslocal circulationsystemic circulationovarian veinsварикоз вен малого тазаженщиныиммунитетиммуноглобулинылокальный кровотоксистемный кровотокяичниковые вены[Головина В.И., Селиверстов Е.И., Ефремова О.И., Золотухин И.А. Роль цитокинов в патогенезе варикозной болезни // Флебология. 2021. Т. 15, № 2. C. 117–126. [Golovina V.I., Seliverstov E.I., Efremova O.I., Zolotukhin I.A. Cytokines in pathogenesis of varicose veins. Flebologiya = Flebologiya, 2021,vol. 15, no. 2, pp. 117–126. 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