Email this article OUTBREAK OF DENGUE VIRUS SEROTYPES IN DENGUE HEMORRHAGIC FEVER PATIENTS OF PESHAWAR PAKISTAN
- Authors: Rehman F.1,2, Khan S.2, Hassan F.1,3, Khan H.2, Ahmad I.4, Khan Z.5
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Affiliations:
- Government Superior Science College Peshawar, KP, 25000 Pakistan
- Department of Zoology University of Peshawar, KP, 25000 Pakistan
- Department of Microbiology, Kohat University of Science and Technology, KP, Kohat 26000, Pakistan
- Harbin Veterinary Research Institute, CAAS- Michigan State University Joint Laboratory of Innate Immunity Key Laboratory of Veterinary Biotechnology, Chinese academy of Agricultural sciences, Harbin China
- Government Superior Science College Peshawar
- Section: ORIGINAL ARTICLES
- URL: https://iimmun.ru/iimm/article/view/2062
- DOI: https://doi.org/10.15789/2220-7619-OOD-2062
- ID: 2062
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Abstract
Abstract
Background: Dengue is the most critical medical problem in tropical and sub-tropical locales of the world transmitted by the bite of the mosquito Aedes aegypti. Dengue virus (DENV) is categorized into 4 serotypes i.e. DENV1, DENV2, DENV3, and DENV4. The role of dengue virus (DENV) serotypes in the causation of primary Dengue hemorrhagic fever (DHF) among dengue patients are not well studied in Pakistan. This research aimed to determine the association of DENV serotypes with DHF in dengue hemorrhagic fever patients of district Peshawar Pakistan.
Methods: A total of 200 blood samples were collected from DF and DHF patients. All of the samples were considered to be positive by NS1 Elisa. RNA was extracted with an RNA kit (TRIzol, USA) using the manufacturer protocol. The extracted RNA was then converted into cDNA by using Revert AID Reverse transcriptase (Thermofisher scientific) the target region for transcription and Amplification is a c-prM region which was amplified by Regular PCR and by using Nested PCR, the DENV serotypes (1-4) (Thermofisher scientific) was identified by using serotypes specific primers (TS1, TS2, TS3, and TS4) the samples was run through gel electrophoresis and then read the product size.
Results: a total of 164 (82%) samples were considered to be positive by both NS1 Elisa and RT-PCR while 36 (18%) were negative by RT-PCR. the study population consists of 60% male and 40% female. Within positive samples, 40 patients have DHF of which 24 (15%) were male and 16 (10%) were females. Most of the affected patients have an age range between 10-30 years followed by 30-40 years. All of the DHF patients were primary patients and had no previous dengue fever history. By using Serotype-specific primers all the DHF samples were positive for Serotype-2 (DENV2). This shows that DENV2 was primarily associated with DHF
Conclusion: DENV2 is the dominant serotype in the Dengue outbreak in affected patients of Peshawar.
About the authors
Faiz Ur Rehman
Government Superior Science College Peshawar, KP, 25000 Pakistan;Department of Zoology University of Peshawar, KP, 25000 Pakistan
Email: faiz02140@gmail.com
ORCID iD: 0000-0001-5970-9346
ResearcherId: Zoology/Virology
Im Mr Faiz Ur Rehman Belongs to Peshawar Pakistan working as a lecturer in Zoology at Government Superior Science College Peshawar Pakistan
I have 5 years teaching and research experience
Pakistan, Government Superior Science College Peshawar Affiliated with University of Peshawar PakistanSana ullah Khan
Department of Zoology University of Peshawar, KP, 25000 Pakistan
Email: sanaullahkhan@uop.edu.pk
ResearcherId: Virology
Associate Professor Department of zoology University of Peshawar Pakistan (20 years)
Pakistan, Department Of Zoology University of Peshawar PakistanFaaiz ul Hassan
Government Superior Science College Peshawar, KP, 25000 Pakistan;Department of Microbiology, Kohat University of Science and Technology, KP, Kohat 26000, Pakistan
Email: fhassan.mb@gmail.com
ResearcherId: Microbiology
PhD Scholar Department of Microbiology Kohat University of Science and Technology (3 years)
Pakistan, Department of Microbiology Kohat University of Science and TechnologyHafeez ullah Khan
Department of Zoology University of Peshawar, KP, 25000 Pakistan
Email: hafeez_ullah300@yahoo.com
Department of zoology University of Peshawar Pakistan (5 years)
Pakistan, University of Peshawar PakistanIqbal Ahmad
Harbin Veterinary Research Institute, CAAS- Michigan State University Joint Laboratory of Innate Immunity Key Laboratory of Veterinary Biotechnology, Chinese academy of Agricultural sciences, Harbin China
Email: iqbal_microbiologist@yahoo.com
ResearcherId: Microbiology /Virology
Post Doctorate Research Scholar Harbin Veterinary Research Institute, CAAS- Michigan State University Joint Laboratory of Innate Immunity Key Laboratory of Veterinary Biotechnology, Chinese academy of Agricultural sciences, Harbin China. (5 years)
Pakistan, Harbin Veterinary Research Institute, CAAS- Michigan State University Joint Laboratory of Innate Immunity Key Laboratory of Veterinary Biotechnology, Chinese academy of Agricultural sciences, Harbin ChinaZahoor Khan
Government Superior Science College Peshawar
Author for correspondence.
Email: zahoor303@gmail.com
MPhil zoology Department of Zoology Islamia College University Peshawar Pakistan (3 years)
Pakistan, Islamia College University Peshawar PAkistanReferences
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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).