Detection of international high-risk clones of food-borne pathogens Salmonella and Escherichia coli in the Russian Federation

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Abstract

The use of whole genome sequencing, standard analyze methods and international databases allows to detect international high-risk clones of food-borne pathogens, to assess their evolution and geographical distribution due to international trade of animal food products and farm animals. In Saint-Petersburg, Russian Federation, Salmonella Newport, Salmonella Kentucky and Escherichia coli O26:H11 of well-known international high-risk clones were first identified from patients with diarrhea. Two S. Kentucky strains isolated in 2015 and 2019 were identical to the international clone ST198 widely distributed in European countries: had multidrug resistance, high-level fluoroquinolone resistance (MIC of ciprofloxacin  >  32,0 mg/l) due to three single-nucleotide substitutions in the gyrA (Ser83Phe and Asp87Asn) and parC (Ser80Ile). In 2008, a multidrug resistant S. Newport strain resistant to extended-spectrum cephalosporins due to AmpC-cephalosporinase CMY-2 was isolated, belonging to the international clone of S. Newport MDR-AmpC/CMY-2  which caused sporadic cases and outbreaks in the USA and Europe in the 2000s. The plasmid containing the blaCMY-2 had a PstI-restriction profile, one of the most prevalent in the international clone. E. coli O26:H11 strains, isolated in Saint-Petersburg,  produced the shiga-like toxin STX1 (stx1a), had additional virulence genes: ehxA (enterohemolysin), katP (catalase peroxidase), espP (serine protease), as well as cba (colicin B), gad (glutamate decarboxylase), cif (type III secreted effector), iss (increased serum survival), belonged to the phylogenetic group B1 and the international high-risk clone  E. coli O26:H11 ST21 widely distributed in Europe and the USA. 25% of the strains had multidrug resistance and produced CTX-M extended spectrum beta-lactamases. In the Russian Federation E. coli O26 is consider as the pathogen of diarrheal diseases and registered as Enteropathogenic E. coli in routine bacteriological laboratories without detecting of H-antigen and shiga-like toxins.

About the authors

L. A. Kaftyreva

Saint-Petersburg Pasteur Institute;
North-Western State Medical University named after I.I. Mechnikov

Author for correspondence.
Email: kaflidia@mail.ru
ORCID iD: 0000-0003-0989-1404

Kaftyreva Lidiia A., PhD, MD (Medicine), Professor, Head of the Laboratory of Enteric Infections, St. Petersburg Pasteur Institute; Professor of the Department of Medical Microbiology, North-Western State Medical University named after I.I. Mechnikov, Ministry of Health of the Russian Federation

197101, St. Petersburg, Mira str., 14

Scopus Author ID: 6602939287

Russian Federation

S. A. Egorova

Saint-Petersburg Pasteur Institute

Email: egorova72@mail.ru
ORCID iD: 0000-0002-7589-0234

Egorova Svetlana A., PhD (Medicine), Senior Researcher, Laboratory of Enteric Infections

St. Petersburg

SPIN-code: 4000-4122

Scopus Author ID: 36937138200 

Russian Federation

M. A. Makarova

Saint-Petersburg Pasteur Institute;
North-Western State Medical University named after I.I. Mechnikov

Email: makmaria@mail.ru
ORCID iD: 0000-0003-3600-2377

Makarova Maria A., PhD (Medicine), Senior Researcher, Laboratory of Pathogen Identification, St. Petersburg Pasteur Institute; Assistant Professor of the Department of Medical Microbiology, North-Western State Medical University named after I.I. Mechnikov, Ministry of Health of the Russian Federation

St. Petersburg

SPIN-code: 7915-1758

Scopus Author ID: 38761767300

Russian Federation

References

  1. Макарова М.А., Дмитриев А.В., Матвеева З.Н., Кафтырева Л.А. Молекулярно-генетическая характеристика штаммов Escherichia coli серогруппы О26, вызывающих диарейные заболевания у детей // Медицинский академический журнал. 2018. Т. 18, № 3. С. 85–90. doi: 10.17816/MAJ18385-90
  2. Bielaszewska M., Mellmann A., Bletz S., Zhang W., Köck R., Kossow A., Prager R., Fruth A., Orth-Höller D., Marejková M., Morabito S., Caprioli A., Piérard D., Smith G., Jenkins C., Curová K., Karch H. Enterohemorrhagic Escherichia coli O26:H11/H-: a new virulent clone emerges in Europe. Clin. Infect. Dis., 2013, vol. 56, no. 10, pp. 1373–1381. doi: 10.1093/cid/cit055
  3. Brooks J.T., Sowers E.G., Wells J.G., Greene K.D., Griffin P.M., Hoekstra R.M., Strockbine N.A. Non-O157 Shiga toxinproducing Escherichia coli infections in the United States, 1983–2002. J. Infect. Dis., 2005, vol. 192, no. 8, pp. 1422–1429. doi: 10.1086/466536
  4. Crim S., Chai S., Karp B., Judd M., Reynolds J., Swanson K., Nisler A., McCullough A., Gould H. Salmonella enterica serotype Newport infections in the US, 2004–2013: increased incidence investigated through four surveillance systems. Foodborne Pathog. Dis., 2018, vol. 15, no. 10, pp. 612–620. doi: 10.1089/fpd.2018.2450
  5. Dallenne C., Da Costa A., Decré D., Favier C., Arlet G. Development of a set of multiplex PCR assays for the detection of genes encoding important beta-lactamases in Enterobacteriaceae. J. Antimicrob. Chemother., 2010, vol. 65, no. 3, pp. 490–495. doi: 10.1093/jac/dkp498
  6. Egorova S., Timinouni M., Demartin M., Granier S.A., Whichard J.M., Sangal V., Fabre L., Delaune A., Pardos M., Millemann Y., Espie E., Achtman M., Grimont P.A.D., Weill F.-X. Ceftriaxone-resistant Salmonella enterica serotype Newport, France. Emerg. Infect. Dis., 2008, vol. 14, no. 6, pp. 954–957. doi: 10.3201/eid1406.071168
  7. European food safety authority and european centre for disease prevention and control the European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2015. EFSA J., 2016, vol. 14, no. 12: e04634. doi: 10.2903/j.efsa.2016.4634
  8. Gonzalez-Escalona N., Toro M., Rump L.V., Cao G., Nagaraja T.G., Meng J. Virulence gene profiles and clonal relationships of Escherichia coli O26:H11 isolates from feedlot cattle as determined by whole-genome sequencing. Appl. Environ. Microbiol., 2016, vol. 82, no. 13, pp. 3900–3912. doi: 10.1128/AEM.00498-16
  9. Hawkey J., Le Hello S., Doublet B., Granier S.A., Hendriksen R.S., Fricke W.F., Ceyssens P.J., Gomart C., Billman-Jacobe H., Holt K.E., Weill F.X. Global phylogenomics of multidrug-resistant Salmonella enterica serotype Kentucky ST198. Microb. Genetics, 2019, vol. 5, no. 7: e000269. doi: 10.1099/mgen.0.000269
  10. Nakaya H., Yasuhara A., Yoshimura K., Oshihoi Y., Izumiya H., Watanabe H. Life-threatening infantile diarrhea from fluoroquinolone-resistant Salmonella enterica Typhimurium with mutations in both gyrA and parC. Emerg. Infect. Dis., 2003, vol. 9, no. 2, pp. 255–257. doi: 10.3201/eid0902.020185
  11. Ogura Y., Gotoh Y., Itoh T., Sato M.P., Seto K., Yoshino S., Isobe J., Etoh Y., Kurogi M., Kimata K., Maeda E., Piérard D., Kusumoto M., Akiba M., Tominaga K., Kirino Y., Kato Y., Shirahige K., Ooka T., Ishijima N., Lee K.I., Iyoda S., Mainil J.G., Hayashi T. Population structure of Escherichia coli O26 : H11 with recent and repeated stx2 acquisition in multiple lineages. Microb. Genomics, 2017, vol. 3, no. 11: e000141. doi: 10.1099/mgen.0.000141
  12. The European Union summary report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2017. EFSA J., 2019, vol. 17, no. 2: e05598. doi: 10.2903/j.efsa.2019.5598
  13. Tobias J., Vutukuru S.R. Simple and rapid multiplex PCR for identification of the main human diarrheagenic Escherichia coli. Microbiol. Res., 2012, vol. 167, no. 9, pp. 564–570. doi: 10.1016/j.micres.2011.11.006
  14. Varma J.K., Marcus R., Stenzel S.A., Hanna S.S., Gettner S., Anderson B.J., Hayes T., Shiferaw B., Crume T.L., Joyce K., Fullerton K.E., Voetsch A.C., Angulo F.J. Highly resistant Salmonella Newport-MDR AmpC transmitted through the domestic US food supply: a FoodNet case-control study of sporadic Salmonella Newport infections, 2002–2003. J. Infect. Dis., 2006, vol. 194, no. 2, pp. 222–230. doi: 10.1086/505084

Copyright (c) 2020 Kaftyreva L.A., Egorova S.A., Makarova M.A.

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