Functional activity of peripheral blood monocytes in patients with opisthorchiasis coupled to liver fibrosis intensity

Cover Page

Cite item

Abstract

The aim is to examine activity of peripheral blood monocytes from patients with opisthorchiasis coupled to intensity of liver fibrosis. Materials and Methods. A total of 74 patients with chronic opisthorchiasis (39 males and 35 females, average age 42.3 years) and 32 apparently healthy subjects (17 males and 15 females, average age 41.5 years) aged 24 to 60 years were enrolled. Opisthorchiasis was mainly diagnosed by parasitological assays to detect adult parasite eggs or bodies in duodenal contents and/or feces in all 74 patients. Liver fibrosis was assessed by elastometry according to the METAVIR scale. Functional activity of peripheral blood monocytes was performed in all patients with opisthorchiasis and healthy individuals from the control group by chemiluminescent analysis to measure intensity of reactive oxygen species production in lucigenin- and luminol-dependent spontaneous and zymosan-induced reactions. Results. Liver fibrosis F2 and F3—F4 stage according to METAVIR scale was found in 20.3% and 17.6% of patients with opisthorchiasis, respectively. While analyzing total pool of reactive oxygen species in the luminol-dependent process in patients with opisthorchiasis, a significantly decreased monocyte functional activity was observed as compared to healthy subjects that was evidenced by significantly decreased maximum intensity of produced reactive oxygen species as well as area under the chemiluminescence curve both in spontaneous and zymosan-induced reaction. Such parameters in liver fibrosis F3—F4 compared to F0—F1 in zymosan-induced response were lowered. Monocyte functional activity in spontaneous luminol-dependent reaction did not differ significantly depending on liver fibrosis intensity in patients with opisthorchiasis. The phagocytosis activation index in patients with opisthorchiasis with liver fibrosis F3—F4 compared to F0—F1 and F2 stage was lower. Similar changes were observed in the lucigenin-dependent reaction. Conclusion. The data obtained undoubtedly provide promising evidence to interpret the mechanisms behind liver fibrosis in patients with opisthorchiasis and create new opportunities for development of diagnostic and therapeutic technologies.

About the authors

V. V. Tsukanov

Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS

Author for correspondence.
Email: gastro@impn.ru
ORCID iD: 0000-0002-9980-2294

Vladislav V. Tsukanov - PhD, MD (Medicine), Professor, Head of the Clinical Department of the Digestive System Pathology of Adults and Children, Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS.

660022, Krasnoyarsk, Partizan Zheleznyak str., 3-G.

Phone: +7 (391) 256-81-71; Fax: +7 (391) 228-06-83

SPIN: 2529-9513

Russian Federation

E. G. Gorchilova

Private Healthcare Institution "Clinical hospital "RZD-Medicine" of Krasnoyarsk city

Email: yourself.15@mail.ru

Gastroenterologist, Gastroenterological Department, Private Healthcare Institution Clinical hospital “RZD-Medicine” of Krasnoyarsk city.

Krasnoyarsk.

Russian Federation

O. A. Kolenchukova

Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS

Email: kalina-chyikova@mail.ru
ORCID iD: 0000-0001-9552-447X

PhD, MD (Biology), Leading Researcher, Laboratory of Molecular and Cellular Physiology and Pathology, Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS.

Krasnoyarsk.

SPIN: 8008-5580

Russian Federation

I. I. Gvozdev

Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS

Email: leshman-mult@mail.ru

Junior Researcher, Laboratory of Molecular and Cellular Physiology and Pathology, Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS.

Krasnoyarsk.

SPIN: 6203-4651

Russian Federation

A. A. Savchenko

Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS

Email: aasavchenko@yandex.ru

PhD, MD (Medicine), Professor, Head of the Laboratory of Molecular and Cellular Physiology and Pathology, Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS.

Krasnoyarsk.

SPIN: 3132-8260

Russian Federation

A. V. Vasyutin

Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS

Email: alexander@kraslan.ru
ORCID iD: 0000-0002-6481-3196

PhD (Medicine), Senior Researcher, Clinical Department of the Digestive System Pathology of Adults and Children, Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS.

Krasnoyarsk.

SPIN: 4075-4538

Russian Federation

J. L. Tonkikh

Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS

Email: tjulia@bk.ru
ORCID iD: 0000-0001-7518-1895

PhD (Medicine), Leading Researcher, Clinical Department of the Digestive System Pathology of Adults and Children, Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS.

Krasnoyarsk.

SPIN: 3292-9128

Russian Federation

O. S. Rzhavicheva

Private Healthcare Institution "Clinical hospital "RZD-Medicine" of Krasnoyarsk city

Email: ros315@yandex.ru

PhD (Medicine), Head of the Gastroenterological Department, Private Healthcare Institution Clinical hospital “RZD-Medicine” of Krasnoyarsk city.

Krasnoyarsk.

Russian Federation

A. G. Borisov

Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS

Email: 2410454@mail.ru
ORCID iD: 0000-0002-9026-2615

PhD (Medicine), Leading Researcher, Laboratory of Molecular and Cellular Physiology and Pathology, Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS.

Krasnoyarsk.

SPIN: 9570-2254

Russian Federation

References

  1. Цуканов В., Горчилова Е., Васютин А., Тонких Ю., Ржавичева О. Современные принципы ведения больных с описторхозом // Врач. 2019. Т. 30, № 12. С. 25-28. doi: 10.29296/25877305-2019-12-07
  2. Цуканов В.В., Тонких Ю.Л., Гилюк А.В., Васютин А.В., Коленчукова О.А., Ржавичева О.С., Борисов А.Г., Савченко А.А., Масленникова Н.А. Диагностика, клиника и лечение описторхоза // Доктор.Ру. 2019. № 8 (163). С. 49-53. doi: 10.31550/1727-2378-2019-163-8-49-53
  3. Bernal W., Wendon J. Acute liver failure. N. Engl. J. Med., 2013, vol. 369, no. 26, pp. 2525—2534. doi: 10.1056/NEJMra1208937
  4. Bouvard V., Baan R., Straif K., Grosse Y., Secretan B., El Ghissassi F., Benbrahim-Tallaa L., Guha N., Freeman C., Galichet L., Cogliano V. A review of human carcinogens — Part B: biological agents. The Lancet. Oncology, 2009, vol. 10, no. 4, pp. 321—322. doi: 10.1016/s1470-2045(09)70096-8
  5. Brempelis K.J., Crispe I.N. Infiltrating monocytes in liver injury and repair. Clin. Transl. Immunology, 2016, vol. 5, no. 11: e113. doi: 10.1038/cti.2016.62
  6. Heymann F., Tacke F. Immunology in the liver — from homeostasis to disease. Nat. Rev. Gastroenterol. Hepatol., 2016, vol. 13, no. 2, pp. 88—110. doi: 10.1038/nrgastro.2015.200
  7. Krenkel O., Tacke F. Liver macrophages in tissue homeostasis and disease. Nat. Rev. Immunol., 2017, vol. 17, no. 5, pp. 306—321. doi: 10.1038/nri.2017.11
  8. Pellicoro A., Aucott R.L., Ramachandran P., Robson A.J., Fallowfield J.A., Snowdon V.K., Hartland S.N., Vernon M., Duffield J.S., Benyon R.C., Forbes S.J., Iredale J.P. Elastin accumulation is regulated at the level of degradation by macrophage metalloelastase (MMP-12) during experimental liver fibrosis. Hepatology, 2012, vol. 55, no. 6, pp. 1965—1975. doi: 10.1002/hep.25567
  9. Poynard T., Bedossa P., Opolon P. Natural history of liver fibrosis progression in patients with chronic hepatitis C. The OBSVIRC, METAVIR, CLINIVIR, and DOSVIRC groups. Lancet, 1997, vol. 349, no. 9055,pp. 825-832. doi: 10.1016/s0140-6736(96)07642-8
  10. Pradere J.P., Kluwe J., De Minicis S., Jiao J.J., Gwak G.Y., Dapito D.H., Jang M.K., Guenther N.D., Mederacke I., Friedman R., Dragomir A.C., Aloman C., Schwabe R.F. Hepatic macrophages but not dendritic cells contribute to liver fibrosis by promoting the survival of activated hepatic stellate cells in mice. Hepatology, 2013, vol. 58, no. 4, pp. 1461-1473. doi: 10. 1002/hep.26429
  11. Prueksapanich P., Piyachaturawat P., Aumpansub P., Ridtitid W., Chaiteerakij R., Rerknimitr R. Liver fluke-associated biliary tract cancer. Gut Liver, 2018, vol. 12, no. 3, pp. 236-245. doi: 10.5009/gnl17102
  12. Schuppan D., Kim Y.O. Evolving therapies for liver fibrosis. J. Clin. Invest., 2013, vol. 123, no. 5, pp. 1887-1901. doi: 10.1172/JCI66028
  13. Sripa B., Kaewkes S., Intapan P.M., Maleewong W., Brindley P.J. Food-borne trematodiases in Southeast Asia epidemiology, pathology, clinical manifestation and control. Adv. Parasitol., 2010, vol. 72, pp. 305-350. doi: 10.1016/S0065-308X(10)72011-X
  14. Strnad P., Tacke F., Koch A., Trautwein C. Liver — guardian, modifier and target of sepsis. Nat. Rev. Gastroenterol. Hepatol., 2017, vol. 14, no. 1, pp. 55- 66. doi: 10.1038/nrgastro.2016.168
  15. Tacke F., Zimmermann H.W. Macrophage heterogeneity in liver injury and fibrosis. J. Hepatol., 2014, vol. 60, no. 5, pp. 10901096. doi: 10.1016/j.jhep.2013.12.025
  16. Triantafyllou E., Woollard K.J., McPhail M.J.W., Antoniades C.G., Possamai L.A. The role of monocytes and macrophages in acute and acute-on-chronic liver failure. Front. Immunol., 2018, vol. 9: 2948. doi: 10.3389/fimmu.2018.02948

Supplementary files

There are no supplementary files to display.


Copyright (c) 2020 Tsukanov V.V., Gorchilova E.G., Kolenchukova O.A., Gvozdev I.I., Savchenko A.A., Vasyutin A.V., Tonkikh J.L., Rzhavicheva O.S., Borisov A.G.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies