EFFECTIVENESS OF MOLECULAR-GENETIC DIAGNOSTICS DURING PERTUSSIS INFECTION FOCI EXAMINATION

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Abstract

Purpose: whooping cough diagnostics by molecular-genetic methods.

Materials and methods. Under observation there were 4930 people during the period from 2012 to 2015. All samples were received in 8 schools of Moscow and the Moscow region: in 3 schools had been found children with whooping cough, in other 5 schools – only children with prolonged cough. Whooping cough diagnosis had been given earlier by bacteriological and serological methods. 430 clinical samples were studied by 2 methods: PCR with fluorescent hybridized detection of amplified products and isothermal amplification.

Results. In three of eight schools whooping cough cases at 7 children at the age of 7, 9, 11 and 15 years were revealed earlier. The diagnosis of whooping cough at them was confirmed by means of bacteriological and serological methods. 33 positive DNA samples (7.7%) are revealed. DNA-positive samples are allocated from 18 pupils and 15 employees of schools. In two schools where earlier infection sources were established, 15 DNA-positive samples from which in three cases clinical manifestations were observed are revealed. In those schools where it wasn’t earlier established a source of an infection and examinations conducted it is long the coughing children, 18 DNA-positive samples are revealed, and in two cases clinical manifestations in the form of cough were observed.

Conclusion. Performed research confirmed high efectiveness of molecular-genetic methods during pertussis infection foci examination in schools for infection source identification also amongst long coughing children. 

About the authors

A. S. Pimenova

G.N. Gabrichevsky Institute of Epidemiology and Microbiology

Email: fake@neicon.ru

Junior Researcher, Laboratory of Diagnostics of Diphtheria and Pertussis Infections,

Moscow

Russian Federation

O. Yu. Borisova

G.N. Gabrichevsky Institute of Epidemiology and Microbiology;
The Russian National Research Medical University named after N.I. Pirogov

Author for correspondence.
Email: olgborisova@mail.ru

PhD, MD (Medicine), Associate Professor, Head of Laboratory of Diagnostics of Diphtheria and Pertussis Infections, 125212, Moscow, Admiral Makarov str., 10;

Professor of Department of Microbiology and Virology,

Moscow

Russian Federation

O. V. Tsvircun

G.N. Gabrichevsky Institute of Epidemiology and Microbiology

Email: fake@neicon.ru

PhD, MD (Medicine), Head Researcher of Laboratory of Whooping Cough and Measles,

Moscow

Russian Federation

A. S. Basov

G.N. Gabrichevsky Institute of Epidemiology and Microbiology

Email: fake@neicon.ru

Junior Researcher, Laboratory of Whooping Cough and Measles,

Moscow

Russian Federation

V. A. Aleshkin

G.N. Gabrichevsky Institute of Epidemiology and Microbiology

Email: fake@neicon.ru

PhD, MD (Medicine), Professor, Director,

Moscow

Russian Federation

S. S. Afanasiev

G.N. Gabrichevsky Institute of Epidemiology and Microbiology

Email: fake@neicon.ru

PhD, MD (Medicine), Professor, Deputy Director for Biotechnology Department,

Moscow

Russian Federation

E. E. Donskich

The Russian National Research Medical University named after N.I. Pirogov

Email: fake@neicon.ru

PhD (Biology), Associate Professor, Associate Professor of Department of Microbiology and Virology,

Moscow

Russian Federation

A. P. Pikina

The Russian National Research Medical University named after N.I. Pirogov

Email: fake@neicon.ru

Assistant Professor, Department of Microbiology and Virology,

Moscow

Russian Federation

L. I. Kafarskaya

The Russian National Research Medical University named after N.I. Pirogov

Email: fake@neicon.ru

PhD, MD (Medicine), Professor, Head of the Department of Microbiology and Virology,

Moscow

Russian Federation

M. S. Afanasiev

Sechenov Moscow State Medical University

Email: fake@neicon.ru

PhD, MD (Medicine), Professor of the Department of Clinical Allergology and Immunology,

Moscow

Russian Federation

A. V. Karaulov

Sechenov Moscow State Medical University

Email: fake@neicon.ru

RAS Full Member, PhD, MD (Medicine), Professor, Head of the Department of Clinical Allergology and Immunology,

Moscow

Russian Federation

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Copyright (c) 2017 Pimenova A.S., Borisova O.Y., Tsvircun O.V., Basov A.S., Aleshkin V.A., Afanasiev S.S., Donskich E.E., Pikina A.P., Kafarskaya L.I., Afanasiev M.S., Karaulov A.V.

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