THE CHANGES OF OF CELLULAR IMMUNITY PARAMETERS IN PATIENTS WITH PREINVASIVE AND MICROINVASIVE CERVICAL CANCER BEFORE AND AFTER TREATMENT

Cover Page


Cite item

Full Text

Abstract

Abstract. The comparative study of immunocompetent cells’ phenotype was performed in patients with the preinvasive and microinvasive cervical cancer before and after the treatment. Besides, the study of importance of selected cellular immunity indices as a predicitive factors of effective surgical treatment in a combination with immunotherapy (the study group) in comparison with a surgical treatment only (the control group) have been conducted. The true numerical normalization of subpopulation composition of peripheral blood lymphocyte has been determined including the expression level of suppressor lymphocytes molecular markers (FOXP3 и TGF-β1) and the expression level of CD95-marker in a study group in comparison with a control group. Moreover, true decrease of HPV (human papillomavirus) — positive patients (1.7%) and the absence of E7 oncoprotein in the study group have been discovered.

About the authors

P. I. Kovchur

Federal state-financed educational institution of high professional education «Petrozavodsk State University», Petrozavodsk

Author for correspondence.
Email: pkovchur@mail.ru

PhD (Medicine), Associate Professor, Department of Radiation Diagnosics, Therapy, Oncology, Tuberculosis and Urology

185910, Russian Federation, Petrozavodsk, Lenina pr., 33

 

Россия

E. V. Bahidze

Federal state-financed institution «N.N. Petrov Research Institute of Oncology» of Russian Ministry of Health, St. Petersburg

Email: pkovchur@mail.ru
PhD, MD (Medicine), Leading Researcher, Department of Oncogynecology Россия

A. V. Yastrebova

Federal state-financed educational institution of high professional education «Petrozavodsk State University», Petrozavodsk

Email: pkovchur@mail.ru
Assistant Professor, Department of Radiation Diagnosics, Therapy, Oncology, Tuberculosis and Urology Россия

References

  1. Бахидзе Е.В., Лавринович О.Е., Чепик О.Ф., Киселев О.И. Роль вируса папилломы человека в лимфогенном метастазировании плоскоклеточного рака шейки матки // Вопросы онкологии. — 2011. — Т. 57, № 3. — С. 318–321. Bakhidze E.V., Lavrinovich O.E., Chepik O.F., Kiselev O.I. Rol` virusa papillomy cheloveka v limfogennom metastazirovanii ploskokletochnogo raka sheyki matki [The role of human papillomavirus in the lymphoid metastasis of squamous cell carcinoma of the cervix]. Voprosy onkologii — Problems of Oncology, 2011, vol. 57, no. 3, pp. 318–321.
  2. Кадагидзе З.Г., Черткова А.И., Славина Е.Г., Заботина Е.Н., Борунова А.А., Короткова О.В. Фенотип иммунокомпетентных клеток и его значение в противоопухолевом иммунном ответе // Вестник РАМН. — 2011. — № 12. — С. 21–25. Kadagidze Z.G., Chertkova A.I., Slavina E.G., Zabotina E.N., Borunova A.A., Korotkova O.V. Fenotip immunokompetentnykh kletok i ego znachenie v protivoopukholevom immunnom otvete [The Phenotype of immune cells and its significance in the antitumor immune response]. Vestnik RAMN — Herald of the Russian Academy of Medical Sciences, 2011, no. 12, pp. 20–25.
  3. Киселев В.И., Свешников П.Г., Барановский П.М., Липова Е.В., Глазко И.И., Ашрафян Л.А. Онкобелок Е7 вируса папилломы человека — новый маркер ранних стадий канцерогенеза // Terra medica. — 2011. — № 1. — С 35–39. Kiselev V.I., Sveshnikov P.G., Baranovskiy P.M., Lipova E.V., Glazko I.I., Ashrafyan L.A. Onkobelok E7 virusa papillomy cheloveka — novyy marker rannikh stadiy kantserogeneza [E7 oncoprotein of human papilloma virus — a new marker of early stages of carcinogenesis]. Terra medica, 2011, no. 1, pp. 35–39.
  4. Короленкова Л.И. Снижение вирусной нагрузки, определенной методом гибридного захвата, у больных тяжелыми интраэпителиальными неоплазиями шейки матки, как результат эффективной предэксцизионной терапии аллокином-альфа // Акушерство и гинекология. — 2012. — № 4–2. — С. 78–82. Korolenkova L.I. Snizhenie virusnoy nagruzki, opredelennoy metodom gibridnogo zakhvata, u bol`nykh tyazhelymi intraepitelial`nymi neoplaziyami sheyki matki, kak rezul`tat effektivnoy predekstsizionnoy terapii allokinom-al`fa [The Reduction in viral load determined by hybrid capture in patients with severe cervical intraepithelial neoplasia, as a result of effective preexcisive therapy by Allokin alpha]. Akusherstvo i ginekologiya — Obstetrics and Gynecology, 2012, no. 4–2, pp. 78–82.
  5. Орнер И.Ю., Абрамовских О.С., Батурина И.Л., Зотова М.А., Никушкина К.В., Телешева Л.Ф. Сравнительный анализ иммунологических показателей периферической крови у пациенток с тяжелой дисплазией и раком шейки матки, ассоциированных с вирусом папилломы человека // Российский аллергологический журнал. — 2010. — № 5 (1). — С. 212–213. Orner I.Yu., Abramovskikh O.S., Baturina I.L., Zotova M.A., Nikushkina K.V., Telesheva L.F. Sravnitel`nyy analiz immunologicheskikh pokazateley perifericheskoy krovi u patsientok s tyazheloy displaziey i rakom sheyki matki, assotsiirovannykh s virusom papillomy cheloveka [Comparative analysis of immunological parameters in peripheral blood in patients with severe dysplasia and cervical cancer associated with human papillomavirus]. Rossiyskiy allergologicheskiy zhurnal — Russian Journal of Allergy, 2010, no. 5, vol. 1, pp. 212–213.
  6. Bolpetti A., Silva J.S., Villa L.L., Lepique A.P. Interleukin-10 production by tumor infiltrating macrophages plays a role in Human Papillomavirus 16 tumor growth. BMC Immunol., 2010, vol. 11, p. 27.
  7. Buckner J.H. Mechanisms autoimmune diseases. Nat. Rev. Immunol., 2010, vol. 10, pp. 849–859.
  8. Bustin S.A. Quantification of mRNA usi ng real-time reverse transcription P.C.R (RT-PCR): trends and problems. J. Mol. Endocrinol., 2002, vol. 29, pp. 23–39.
  9. Chernysh S.I., Kim S.I., Bekker G., Pleskach V.A., Filatova N.A., Anikin V.B., Platonov V.G., Bulet P. Antiviral and antitumor peptides from insects. Proc. Natl. Acad. Sci. USA, 2002, vol. 9, no. 20, pp. 12628–12632.
  10. Contreras D.N., Krammer, P.H., Potkul, R.K., Bu P., Rossi J.L., Kaufmann A.M., Gissmann L., Qiao L. Cervical cancer cells induce apoptosis of cytotoxic T lymphocytes. J. Immunother., 2000, vol. 23, no. 1, pp. 67–74.
  11. Das S., Karim S., Datta R.C., Maiti A.K., Ghosh S.K., Chaudhury K. Peripheral blood lymphocyte subpopulations in patients with cervical cancer. Int. J. Gynaecol. Obstet., 2007, vol. 98, no. 2, pp.143–146.
  12. Doorbar J. Molecular biology of human papillomavirus infection and cervical cancer. Clin. Sci., 2006, vol. 110, pp. 525–541.
  13. Ibeanu O.A. Molecular pathogenesis of cervical cancer. Cancer Biol. Ther., 2011, vol. 11, no. 3, pp. 295–306.
  14. Kanodia S., Da Silva D.M., Kas, W.M. Recent advances in strategies for immunotherapy of human papillomavirus-induced lesions. Int. J. Cancer, 2008, vol. 122, pp. 247–259.
  15. Loddenkemper C., Hoffmann C., Stanke J., Nagorsen D., Baron U., Olek S., Huehn J., Ritz J.P., Stein H., Kaufmann A.M., Schneider A., Cichon G. Regulatory (FOXP3+) T cells as target for immune therapy of cervical intraepithelial neoplasia and cervical cancer. Cancer Sci., 2009, vol. 100, no. 6, pp. 1112–1117.
  16. Moody C.A., Laimins L.A. Human papillomavirus oncoproteins: pathways to transformation. Nat. Rev. Cancer, 2010, vol. 10, no. 8, pp. 550–560.
  17. Patel S., Chiplunkar S. Host immune responses to cervical cancer. Curr Opin Obstet Gynecol., 2009, vol. 21, no. 1, pp. 54–59.
  18. Sakaguchi S., Miyara M., Costantino C.M., Hafler D.A. FOXP3+ regulatory T cells in the human immune system. Nat. Rev. Immunol., 2010, vol. 10, pp. 490–500.
  19. Stanley M.A. Immune responses to human papilloma viruses. Indian J. Med. Res., 2009, vol. 130, pp. 266–276.
  20. Trimble C.L., Peng S., Thoburn C., Kos F., Wu T.C. Naturally occurring systemic immune responses to H.P.V antigens do not predict regression of C.I.N2/3. Cancer Immunol. Immunother., 2010, no. 59, pp. 799–803.
  21. Visser J., Nijman H.W., Hoogenboom B.N., Jager P., van Baarle D., Schuuring E., Abdulahad W., Miedema F., van der Zee A.G., Daemen T. Frequencies and role of regulatory T cells in patients with (pre)malignant cervical neoplasia. Clin. Exp. Immunol., 2007, vol. 150, no. 2, pp. 199–209.
  22. Yang Z.Z., Ansell S.M. The role of Treg cells in the cancer immunological response. Am. J. Immunol., 2009, vol. 5, no. 1, pp. 17–28.
  23. Jayshree R.S., Sreenivas A., Tessy M., Krishna S. Cell intrinsic and extrinsic factors in cervical carcinogenesis. Indian J. Med. Res., 2009, vol. 130, no. 3, pp. 286–295.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2014 Kovchur P.I., Bahidze E.V., Yastrebova A.V.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 64788 от 02.02.2016.