Email this article Prevalence of Borrelia burgdorferi sensu lato genetic markers in blood-sucking ticks in suburban park zones in Saint Petersburg
- Authors: Panferova Y.A.1, Vaganova A.N.1, Freylikhman O.A.1, Tretyakov K.A.2, Medvedev S.G.2, Shapar’ A.O.3, Tokarevich N.K.1
-
Affiliations:
- St. Petersburg Pasteur Institute
- Zoological Intstitute, RAS
- Center for State Sanitary and Epidemiological Surveillance in St. Petersburg
- Issue: Vol 10, No 1 (2020)
- Pages: 175-179
- Section: SHORT COMMUNICATIONS
- URL: https://iimmun.ru/iimm/article/view/806
- DOI: https://doi.org/10.15789/2220-7619-POB-806
- ID: 806
Cite item
Full Text
Abstract
Tick-borne bacterial and viral infections are widespread in middle latitudes of the Northern hemisphere. Natural foci of such infections coincide with geographic areas inhabited by ixodid ticks. Ixodid tick-borne borreliosis is a pressing issue for some territories of Russia, especially for the North-Western Federal District and St. Petersburg megalopolis as well as adjacent areas of the Leningrad District, where people may become infected after tick bite in recreational zones in suburban park areas. Currently, very few publications regarding prevalence of tick-borne pathogens in St. Petersburg area are available. In our study, questing ticks flagged in park zone (northern coast of Finnish Gulf, Kurortny District) were examined with PCR for carriage of pathogenic B. burgdorferi sensu lato complex. In addition, samples positive for Borrelia DNA signal were further genotyped with species-specific primers against rpoBgene fragment. It was found that Ixodes persulcatus dominated in this area. Prevalence of Borrelia burgdorferi s.l. complex comprised 9.33%. Genospecies B. afzelii and less frequently B. garinii were detected. A mixt-infection with two Borel-lia species was detected in one sample. Interestingly, all Borrelia-infected ticks belonged to I. persulcatus suggesting a closer association for certain species in «pathogen-vector» system. Our findings are essential in investigating distribution of ixodid borreliosis foci in St. Petersburg and suburbs, obtaining new data regarding epidemiology, diagnostics, treatment and prevention of this infection. It is noteworthy than prevalence of pathogenic Borrelia spp. vs. tick-borne encephalitis virus in vectors was higher thereby accounting for its higher morbidity. Comparing our data with those published elsewhere by European researchers allows to note that prevalence of pathogenic Borrelia spp. in ticks varies broadly in diverse geographic regions. It is necessary to take into consideration that prevalence of Borrelia markers achieves ~10% in ticks given frequent attendance of park areas near St. Petersburg that point at risk of developing bor-reliosis in recreational zones.
Keywords
About the authors
Yu. A. Panferova
St. Petersburg Pasteur Institute
Author for correspondence.
Email: ersvart@inbox.ru
Yulia A. Panferova - Junior Researcher, Laboratory of Zoonoses.
197101, St. Petersburg, Mira str., 14, Phone: +7 (812) 232-21-36 (office), Fax: +7 (812) 233-20-92
Russian FederationA. N. Vaganova
St. Petersburg Pasteur Institute
Email: zoonoses@mail.ru
Researcher, Laboratory of Biomolecular Technologies, Department of New Technologies.
St. Petersburg
Russian FederationO. A. Freylikhman
St. Petersburg Pasteur Institute
Email: zoonoses@mail.ru
PhD (Biology), Head of the Laboratory of Molecular Biological Techniques.
St. Petersburg
Russian FederationK. A. Tretyakov
Zoological Intstitute, RAS
Email: zoonoses@mail.ru
PhD (Biology), Senior Researcher, Laboratory of Parasitology.
St. Petersburg Russian FederationS. G. Medvedev
Zoological Intstitute, RAS
Email: zoonoses@mail.ru
PhD, MD (Biology), Head of Laboratory of Parasitology.
St. Petersburg
Russian FederationA. O. Shapar’
Center for State Sanitary and Epidemiological Surveillance in St. Petersburg
Email: zoonoses@mail.ru
Head of Parasitological Department.
St. Petersburg Russian FederationN. K. Tokarevich
St. Petersburg Pasteur Institute
Email: zoonoses@mail.ru
Tokarevich N.K., PhD, MD (Medicine), Professor, Head of the Laboratory of Zoonoses, St. Petersburg PI; Professor, Department of Epidemiology, North-Western SMU named after I.I. Mechnikov.
St. Petersburg Russian FederationReferences
- Балашов Ю.С., Григорьева Л.А., Оливер Дж.Х. Локализация боррелий в организме клеща Ixodes persulcatus на разных стадиях развития // Паразитология. 1997. Т. 31, № 2. С. 97—103.
- Коротков Ю.С., Кисленко Г.С., Буренкова Л.А., Рудникова Н.А., Карань Л.С. Пространственная и временная изменчивость зараженности клещей Ixodes ricinus и Ixodes persulcatus возбудителем болезни Лайма в Московской области // Паразитология. 2008. Т. 42, № 6. С. 441-451.
- Москвитина Н.С., Коробицын И.Г., Тютеньков О.Ю., Гашков С.И., Кононова Ю.В., Москвитин С.С., Романенко В.Н., Микрюкова Т.П., Протопопова Е.В., Карташов М.Ю., Чаусов Е.В., Коновалова С.Н., Тупота Н.Л., Семенцова А.О., Терновой В.А., Локтев В.Б. Участие птиц в поддержании клещевых инфекций в Томском антропургическом очаге // Известия Российской Академии наук. Серия биологическая. 2014. № 4. С. 408-414.
- Панферова Ю.А., Суворова М.А., Шапарь А.О., Токаревич Н.К. Бактериальные и вирусные патогены в клещах Ixodes sp. на территории Санкт-Петербурга и Ленинградской области // Инфекция и иммунитет. 2018. Т. 8, № 2. С. 219-222.
- Филиппова Н.А. Иксодовые клещи подсемейства Ixodinae. Л.: Наука. Ленингр. отделение, 1977. 393 с.
- Hubalek Z., Halouzka J. Prevalence rates of Borrelia burgdorferi sensu lato in host seeking Ixodes ricinus ticks in Europe. Parasitol. Res., 1998, vol. 84, pp. 167-172.
- Junttila J., Tanskanen R., Tuomi J. Prevalence of Borrelia burgdorferi in selected tick populations in Finland. Scand. J. Infect. Dis, 1994, vol. 26, pp. 349-355
- Junttila J., Peltomaa M., Soini H., Marjamaki M., Viljanen M. Prevalence of Borrelia burgdorferi in Ixodes ricinus ticks in urban recreational areas in Helsinki. J. Clin. Microbiol., 1999, vol. 37, pp. 1361-1365.
- Kalmar Z., Cozma V., Sprong H., Jahfari S., D’Amico G., Marcutan D., Ionica A., Magdas C., Modry D., Mihalca A. Transstadial transmission of Borrelia turcica in Hyalomma aegyptum ticks. PLoS One, 2015, vol. 10: e0115520. doi: 10.1371/journal.pone.0115520
- Kim C., Yi Y., Yu D., Lee M., Ccho M., Desai A., Shringi S., Klein T.A, Kim H.C., Song J.W., Baek L.J., Chong S.T., O’Guinn M.L., Lee J.S., Lee I.Y., Park J.H., Foley J., Chae J.S. Tick-borne rickettsial pathogens in ticks and small mammals in Korea. Appl. Environ. Microbiol., 2006, vol. 72, no. 9,pp. 5766-5776. doi: 10.1128/AEM.00431-06
- Kjelland V., Stuen S., Skarpaas T., Slettan A. Prevalence and genotypes of Borrelia burgdorferi sensu lato infection in Ixodes ricinus ticks in southern Norway. Scand. J. Infect. Dis., 2010, vol. 42, pp. 579-585. doi: 10.3109/00365541003716526
- Laaksonen M., Klemola T., Feuth E., Sormunen J., Puisto A., Makela S., Penttinen R., Ruohomaki K., Hanninen J., Saaksjarvi I., Sprong H., Hytonen J., Vesterinen E. Tick-borne pathogens in Finland: comparison of Ixodes ricinus and I. persulcatus in sym-patric and parapatric areas. Parasites Vectors, 2018, vol. 11: 556. doi: 10.1186/s13071-018-3131-y
- Lee S.H., Kim B.J., Kim J.H., Park K.H., Kim S.J., Kook Y.H. Differentiation of Borrelia burgdorferi sensu lato on the basis of RNA polymerase gene (rpoB) sequences. J. Clin. Microbiol., 2000, vol. 38, pp. 2557-2562.
- Nefedova V., Korenberg E., Gorelova N., Kovalevkii Yu. Studies on the transovarial transmission of Borrelia burgdorferi sensu lato in the taiga tick Ixodes persulcatus. Folia Parasitol., 2004, vol. 51. pp. 67-71.
- Noda A., Rodriguez I., Mondeja B., Fernandez C. Design, оptimization and evaluation of a polymerase chain reaction for detection of Borrelia spp. Adv. Clin. Exp. Med., 2013, vol. 22, pp. 639-653.
- Rogovskyy A., Batool M., Gilis D., Holman P., Nebogatkin I., Rogovska Yu., Rogovskyy M. Diversity of Borrelia spirochetes and other zoonotic agents in ticks from Kyiv, Ukraine. Ticks Tick-borne Dis., 2018, vol. 9, pp. 404-409. doi: 10.1016/j.ttbdis.2017.12.006
- Sormunen J., Penttinen R., Klemola T., Hanninen J., Vuorinen I., Laaksonen M., Saaksjarvi I., Ruohomaki K., Vesterinen E. Tick-borne bacterial pathogen in southwestern Finland. Parasit. Vectors, 2016, vol. 9, pp. 168. doi: 10.1186/s13071-016-1449-x
- Toutoungi L.N, Gern L. Ability of transovarially and subsequent transstadially infected Ixodes hexagonus ticks to maintain and transmit Borrelia burgdorferi in the laboratory. Exp. Appl. Acarol., 1993, vol. 17, pp. 581-586.
Supplementary files
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).