REGULATORY INFLUENCE OF BLOOD MONOCYTES ON THE POPULATION COMPOSITION OF GRANULOCYTES AND THE STATE OF THEIR RESPIRATORY BURST IN THE WIDESPREAD PURULENT PERITONITIS

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  • Authors: Savchenko A.A.1, Borisov A.G.1, Cherdancev D.V.2, Pervova O.V.2, Kudryavcev I.V.3, Belenyuk V.D.4
  • Affiliations:
    1. Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenetsky
    2. Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenetsky
    3. Research Institute of Experimental Medicine Pavlov First St. Petersburg State Medical University
    4. Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North
  • Issue: Vol 8, No 2 (2018)
  • Pages: 201-210
  • Section: ORIGINAL ARTICLES
  • URL: https://iimmun.ru/iimm/article/view/739
  • DOI: https://doi.org/10.15789/2220-7619-2018-2-201-210

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Abstract

The aim of the study was to investigate the regulatory effect of monocytes and their subpopulations on the population composition  of granulocyte leukocytes and the state of their respiratory burst in  widespread purulent peritonitis (WPP). The study involved 24  patients aged 30-65 with acute surgical diseases and injuries of  abdominal organs complicated by WPP. As a control 25 relatively  healthy people of the same age range were examined. A study of the population composition of monocytes and granulocyte leukocytes in  blood was performed using a two-platform technology on the  hematological analyzer Sysmex XE-5000 (Sysmex Inc., USA) and  FC-500 flow cytometer (Beckman Coulter, USA) using the Cytodiff antibody kit (Beckman Coulter, USA). A study of the  monocytes number expressing HLA-DR- and CD64-receptor was performed by flow cytometry using direct immunofluorescence  of whole peripheral blood. The respiratory burst state of neutrophilic granulocytes was studied by chemiluminescence analysis on a 36-channel chemiluminescence analyzer BLM-3607 (MedBioTech, Russia). As indicators of chemiluminescence  were used luminol and lucigenin. The enhancement of  chemiluminescence induced by zymosan was evaluated by the ratio  of the area of the induced chemiluminescence to the spontaneous area and was defined as the activation index. It has  been established that the immune-inflammatory process in WPP is  characterized by a decrease in the number of classical monocytes in  the peripheral blood and an increase in the content of non-classical  monocytes. In WPP in peripheral blood the level of monocytes  expressing HLA-DR receptors decreases. The change in the ratio of  monocytes subpopulations characterizes the increase in the role of  the proinflammatory fraction in the WPP pathogenesis. Changes in  the population composition of granulocytes in the blood in patients  with WPP also characterize the development of an acute  inflammatory process. In this case, there is a decrease in the  number of basophils in the peripheral blood, which, apparently, is  determined by the presence of an allergic component in WPP and,  accordingly, their migration to the inflammation area. In patients  with WPP activation of a respiratory burst of granulocytes of blood  was detected, the intensity of which is determined by the synthesis  of primary and secondary active oxygen species. The results of the  correlation analysis made it possible to establish that in WPP the  regulatory role of non-classical monocytes increases aimed at  stimulating the inflammatory processes (an increase in the number  of mature and immature forms of neutrophils and stimulation of the activity of a respiratory explosion of granulocytes). The revealed features of the regulatory effect of monocytes on the population  composition and the intensity of the respiratory burst of granulocytes can be used in the development of immunotherapeutic methods aimed at reducing the activity of the inflammatory process in WPP.

About the authors

A. A. Savchenko

Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North

Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenetsky

Email: fake@neicon.ru

PhD, MD (Medicine), Professor, Head of Laboratory of  Molecular Cell Physiology and Pathology, Scientific Research  Institute of Medical Problems of the  North, Krasnoyarsk, Russian Federation; Head of the  Department of Physiology, Krasnoyarsk State Medical  University named after Prof. V.F. Voino- Yasenetsky, Krasnoyarsk, Russian Federation

Russian Federation

A. G. Borisov

Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North

Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenetsky

Email: fake@neicon.ru

PhD (Medicine), Leading Researcher, Laboratory of  Molecular Cell Physiology and Pathology, Scientific Research Institute of Medical Problems of the North,  Krasnoyarsk, Russian Federation; Associate Professor,  Department of Infectious Diseases, Krasnoyarsk State  Medical University named after Prof. V.F. Voino- Yasenetsky, Krasnoyarsk, Russian Federation

Russian Federation

D. V. Cherdancev

Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenetsky

Email: fake@neicon.ru

PhD, MD (Medicine), Professor, Head of the Department and  Clinic Surgical Diseases named after prof. A.M. Dychno with the course of endoscopy and endosurgery, Krasnoyarsk State  Medical University named after Prof. V.F. Voino- Yasenetsky, Krasnoyarsk, Russian Federation

Russian Federation

O. V. Pervova

Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenetsky

Email: fake@neicon.ru

PhD, MD (Medicine), Professor of the Department and Clinic  Surgical Diseases named after prof. A.M. Dychno with the  course of endoscopy and endosurgery, Krasnoyarsk State Medical University named after Prof. V.F. Voino- Yasenetsky, Krasnoyarsk, Russian Federation

Russian Federation

I. V. Kudryavcev

Research Institute of Experimental Medicine

Pavlov First St. Petersburg State Medical University

Author for correspondence.
Email: igorek1981@yandex.ru

PhD (Biology), Senior Researcher, Laboratory of  Immunology, Institute of Experimental Medicine, St.  Petersburg, Russian Federation; Associate Professor,  Department of Immunology, Pavlov First St. Petersburg State Medical  University, St. Petersburg, Russian Federation

Russian Federation

V. D. Belenyuk

Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North

Email: fake@neicon.ru

Junior Researcher, Laboratory of Molecular Cell Physiology and  Pathology, Scientific Research Institute of Medical Problems of the North, Krasnoyarsk, Russian Federation

Russian Federation

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Copyright (c) 2018 Savchenko A.A., Borisov A.G., Cherdancev D.V., Pervova O.V., Kudryavcev I.V., Belenyuk V.D.

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