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It is known that any surfaces in environment of healthcare organizations are a reservoir for microorganisms and contribute to transmission of pathogens, increasing the risk of cross-contamination through indirect contact with the patient. Wide and not always rational use of chemical disinfectants carries risks for the safety of the environment, the patient and medical staff. In this regard, there remains the search for alternative methods of purification and decontamination of abiotic surfaces in health care facilities. Tested the tool for cleaning areas that contain 3 strains of the genus Bacillus: B. subtillis, B. licheniformis, B. pumilus. Were treated areas of the medical centre in St. Petersburg Pasteur Institute: the treatment room (experience) and the office of the gynecologist (control with conventional cleaning and disinfection). Within 30 days of the sowing of samples of swabs from surfaces were made of 3 Petri dishes with the “blood” agar-based environment “agar of Givental–Witch” debilitating touch (gold method). Isolated cultures of bacteria identified by microscopy, biochemical typing and MALDI-TOF MS (Bruker). In control and the experience in the 1st day of observation prior to the cleaning sown Enterococcus faecium 104 –105 CFU/ml. The 2nd day of observation in the control was dominated by E. faecium, then his growth stopped. For the 2nd–4th day watched the growth of Enterobacteriaceae to 106 CFU/ml, in the future, the number was 10 CFU/ml until the end of the observation period (30 days). The 5th day in the control revealed the growth of bacteria of the genus Staphylococcus spp. mainly S. epidermidis. The experience from the 2nd day of observation revealed a significant increase in Bacillus spp. at 105 CFU/ml, then before the end of the experiment, the number was 102 –103 CFU/ml. In the 1st–2nd day revealed growth of E. faecium, and then it stopped. For the 2nd–4th day watched the growth of Enterobacteriaceae to the level of 106 CFU/ml, on the 8th day — to 102 CFU/ml, the growth of conditionally pathogenic microflora was absent. Cleaning of the premises on the basis of probiotic bacteria of the genus Bacillus inhibits the growth of sanitary-indicative microorganisms compared to control. Thus, the means of probiotic treatment did not have a biocidal effect, but inhabiting the surface, they inhibit the growth and proliferation of opportunistic bacteria.


About the authors

A. G. Afinogenova

St. Petersburg Pasteur Institute, St. Petersburg;
St. Petersburg State University, St. Petersburg

Author for correspondence.
PhD, MD (Biology), Head of Laboratory Testing Centre, St. Petersburg Pasteur Institute; Professor of Surgical Dentistry Department, St. Petersburg State University Russian Federation

L. A. Kraeva

St. Petersburg Pasteur Institute, St. Petersburg

PhD, MD (Medicine), Head of Clinical Bacteriological Laboratory Russian Federation

G. E. Afinogenov

St. Petersburg State University, St. Petersburg

PhD, MD (Medicine), Professor of Surgical Dentistry Department Russian Federation

V. V. Veretennikov

St. Petersburg State University, St. Petersburg

St. Petersburg State Academy of Veterinary Medicine Student; Trainee of Clinical Bacteriological Laboratory Russian Federation


  1. Коза Н.М. Инфекции, связанные с оказанием медицинской помощи. Эпидемиология и профилактика (обзорная лекция) // Пермский медицинский журнал. 2013. Т. 30, № 3. С. 135–143. [Koza N.M. Infections connected with rendering medical care. epidemiology and prevention (review lecture). Permskii meditsinskii zhurnal = Perm Medical Journal, 2013, vol. 30, no. 3, pp. 135–143. (In Russ.)]
  2. Эпидемиологическое наблюдение за инфекциями, связанными с оказанием медицинской помощи: федеральные клинические рекомендации. Москва, 2014, 58 с. [Epidemiologicheskoe nablyudenie za infektsiyami, svyazannymi s okazaniem meditsinskoi pomoshchi: federal’nye klinicheskie rekomendatsii [Epidemiological surveillance of infections associated with the provision of medical care: federal clinical guidelines]. Moscow, 2014, 58 p. URL: common/2014_7_Epid_nabl_new.pdf (19.12.2017)
  3. Aiello A., Larson E. Antibacterial clearing and hygiene products as an emerging risk factor for antibiotic resistance in the community. Lancet Infect. Dis., 2003, vol. 3, iss. 8, pp. 501–506. doi: 10.1016/S1473-3099(03)00723-0
  4. Burke J.P. Infection control – a problem for patient safety. N. Engl. J. Med., 2003, no. 348, pp. 651–656. doi: 10.1056/NEJMhpr020557
  5. Caselli E. Hygiene: microbial strategies to reduce pathogens and drug resistance in clinical settings. Microb. Biotechnol., 2017, vol. 10, no. 5, pp. 1079–1083. doi: 10.1111/1751-7915.12755
  6. Caselli E., Antonioli P., Mazzacane S. Safety of probiotics used for hospital environmental sanitation. J. Hosp. Inf., 2016, no. 94, iss. 2, pp. 193–194. doi: 10.1016/j.jhin.2016.06.021
  7. Caselli E., D’Accolti M., Vandini A., Lanzoni L., Camerada M.T., Coccagna M., Branchini A., Antonioli P., Balboni P.G., Di Luca D., Mazzacane S. Impact of a probiotic-based cleaning intervention on the microbiota ecosystem of the hospital surfaces: focus on the resistome remodulation. PLoS ONE, 2016, vol. 11, no. 2:e0148857. doi: 10.1371/journal.pone.0148857
  8. Centers for Disease Control and Prevention (CDC). Guidelines for disinfection and sterilization in healthcare facilities. 2008. URL: (19.12.2017)
  9. Dancer S.J. The role of environmental cleaning in the control of hospital-acquired infection. J. Hosp. Infect., 2009, vol. 73, no. 4, pp. 378–385. doi: 10.1016/j.jhin.2009.03.030
  10. Doron S., Snydman D.R. Risk and safety of probiotics. Clin. Infect. Dis., 2015, vol. 60, suppl. 2, pp. S129–S134. doi: 10.1093/cid/ civ085
  11. European Centre for Disease Prevention and Control (ECDC). Point prevalence survey of healthcare-associated infections and antimicrobial use in European acute care hospitals – protocol version 5.3. s. Stockholm: ECDC, 2013. URL: https://ecdc.europa. eu/sites/portal/files/media/en/publications/Publications/healthcare-associated-infections-antimicrobial-use-PPS.pdf (19.12.2017)
  12. Falagas M.E., Makris G.C. Probiotic bacteria and biosurfactants for nosocomial infection control: a hypothesis. J. Hosp. Infect., 2009, vol. 71, no. 4, pp. 301–306. doi: 10.1016/j.jhin.2008.12.008
  13. Hibbing M.E., Fuqua C., Parsek M.R., Peterson S.B. Bacterial competition: surviving and thriving in the microbial jungle. Nat. Rev. Microbiol., 2010, vol. 8, no. 1, pp. 15–25. doi: 10.1038/nrmicro2259
  14. Kramer A., Schwebke I., Kampf G. How long do nosocomial pathogens persist on inanimate surfaces? A systematic review. BMC Infect. Dis., 2006, no. 6, p. 130. doi: 10.1186/1471-2334-6-130
  15. Otter J.A., Yezli S., Salkeld J.A., French G.L. Evidence that contaminated surfaces contribute to the transmission of hospital pathogens and an overview of strategies to address contaminated surfaces in hospital settings. Am. J. Infect. Control, 2013, no. 41, pp. S6–S11. doi: 10.1016/j.ajic.2012.12.004
  16. Vandini A., Frabetti A., Antonioli P., Platano D., Branchini A., Camerada M.T., Lanzoni L., Balboni P., Mazzacane S. Reduction of the microbiological load on hospital surfaces through probiotic-based cleaning procedures: a new strategy to control nosocomial infections. J. Microbiol. Exp., 2014, vol. 1, no. 5:00027. doi: 10.15406/jmen.2014.01.00027
  17. Vandini A., Temmerman R., Frabetti A., Caselli E., Antonioli P., Balboni P.G., Platano D., Branchini A., Mazzacane S. Hard surface biocontrol in hospitals using microbial-based cleaning products. PLoS ONE, 2014, vol. 9, no. 9: e108598. doi: 10.1371/ journal.pone.0108598

Copyright (c) 2018 Afinogenova A.G., Kraeva L.A., Afinogenov G.E., Veretennikov V.V.

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