COMPARATIVE EVALUATION OF CULTURE MEDIA FOR PATHOGEN ISOLATION OF PURULENT BACTERIAL MENINGITIS

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Abstract

The State Research Center for Applied Microbiology and Biotechnology has designed two nutrient media — chocolate agar and PBM-agar to isolate pathogens of purulent bacterial meningitis (PBM). In our previous research using collected microbial strains the media were shown to be highly susceptible and to provide the growth of Neisseria meningiti-dis, Streptococcus pneumoniae and Haemophilus influenzae strains, when inoculated with microbial suspensions containing single cells. When isolating Haemophilus influenzae, meningococci, and pneumococci the use of selective additives in both media assures selective isolation of required microorganisms, inhibiting contaminants. The objective of this research was to assess the media in bacteriological tests of clinical samples collected from the upper and lower respiratory tract in humans. The bacteriological plating of throat smear specimens (n = 90) from children and adults at the age of 0 to 66 with disorder of the upper respiratory tract on chocolate agar, PBM-agar and on a control medium in the absence of selective additives resulted in the equal amount of microbial cultures isolated. Of 154 isolated cultures 2, 23 and 9 were attributed to Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae, respectively. The plating of throat smears (n = 10) from healthy people at the age of 30 to 55 on the analyzable and control media in the presence of additives allowed us to selectively isolate Haemophilus influenzae and Streptococcus pneumoniae cultures without a quantitative loss, with contaminants inhibited. By their growth characteristics chocolate agar and PBM-agar were highly competitive with reference media being used in clinical practice for isolating main causative agents of purulent bacterial meningitis.

About the authors

Ya. V. Podkopaev

State Research Center for Applied Microbiology and Biotechnology, Rospotrebnadzor

Author for correspondence.
Email: podkopaev@obolensk.org
Junior Researcher, Laboratory of Culture Medium Development Russian Federation

L. V. Domotenko

State Research Center for Applied Microbiology and Biotechnology, Rospotrebnadzor

Email: podkopaev@obolensk.org
PhD (Chemistry), Head of the Laboratory of Culture Medium Development Russian Federation

A. N. Kruglov

National Agency for Clinical Pharmacology and Pharmacy

Email: podkopaev@obolensk.org
PhD (Biology), Senior Researcher, Head of the Laboratory of Microbiology Russian Federation

I. V. Ryabchenko

National Agency for Clinical Pharmacology and Pharmacy

Email: podkopaev@obolensk.org
Bacteriologist Russian Federation

K. V. Detushev

State Research Center for Applied Microbiology and Biotechnology, Rospotrebnadzor

Email: podkopaev@obolensk.org
Junior Researcher, Department of Collection Cultures Russian Federation

T. P. Morozova

State Research Center for Applied Microbiology and Biotechnology, Rospotrebnadzor

Email: podkopaev@obolensk.org
Researcher, Laboratory of Culture Medium Development Russian Federation

A. P. Shepelin

State Research Center for Applied Microbiology and Biotechnology, Rospotrebnadzor

Email: podkopaev@obolensk.org
PhD, MD (Biology), Deputy Director Russian Federation

References

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Copyright (c) 2017 Podkopaev Y.V., Domotenko L.V., Kruglov A.N., Ryabchenko I.V., Detushev K.V., Morozova T.P., Shepelin A.P.

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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