GENETIC VARIANTS OF HEPATITIS B VIRUS IN PRIMARY DONORS IN ASTANA, KAZAKHSTAN

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Abstract

The prevalence of one of the hepatotropic virus, hepatitis B virus (HBV) remains a serious global health problem. Since hepatitis B is transmitted through contact with blood or other fluids of an infected person, blood safety is one of the major public health issues in regions with high virus prevalence. Observed in recent years the trend to a shift in the prevalence of various genotypes of HBV in different geographical areas due to immigration from regions of the world with a high incidence of hepatotropic viruses, makes doctors and epidemiologists to pay close attention to the epidemiological situation in neighboring countries. The aim of our work was to study the characteristics of the genetic structure of the HBV in primary donors in Astana, Kazakhstan. A total of 30 blood plasma samples from newly diagnosed hepatitis B (HBsAg+) of Astana. HBV DNA was detected in 27 samples out of 30. Based on the phylogenetic analysis of the isolates showed that among patients examined HBV identified mainly D genotype, which is the most common genotype of HBV in Central Asia. Thus HBV subtype predominant D1 (85,2%) compared to the HBV subtype D2 (3,7%) and subtype D3 (7,4%), in a single sample was detected HBV genotypes A subtype A1. High similarities identified isolates previously described in Iran, Sudan, Mongolia, Tunisia suggest numerous independent, perhaps mutual, the importation of the virus in the country, including in the major migration waves. First detected at the territory of Kazakhstan HBV subtype A1, uncharacteristic for the region, as well as subtypes D2 and D3, which have a high similarity with the nucleotide sequences of HBV in Russia, show cases of importation of the virus from other countries. Identification of the propagation and the role of «imported» genotypes of HBV virus in circulation may be essential for regions where the prevalence of hepatotropic viruses is high, and the genome structure and the way of their distribution sufficiently studied.

About the authors

Yu. V. Ostankova

St. Petersburg Pasteur Institute, St. Petersburg, Russian Federation

Author for correspondence.
Email: shenna1@yandex.ru

Researcher, Laboratory of Molecular Immunology

Russian Federation

A. V. Semenov

St. Petersburg Pasteur Institute, St. Petersburg, Russian Federation
Pavlov First Saint Petersburg State Medical University, St. Petersburg, Russian Federation
North-West State Medical University named after I.I. Mechnikov, St. Petersburg, Russian Federation

Email: shenna1@yandex.ru

PhD (Biology), Head of the Laboratory of Virology and Immunology HIV, St. Petersburg Pasteur Institute; Associate Professor, Pavlov First St. Petersburg State Medical University; Associate Professor, Department of Clinical Laboratory Diagnostics, North-West State Medical University named after I.I. Mechnikov,
St. Petersburg, Russian Federation

Russian Federation

Z. K. Burkitbayev

Research-Production Center of Transfusiology, Astana, Kazakhstan

Email: shenna1@yandex.ru

PhD (Medicine), Director

Kazakhstan

T. N. Savchuk

Research-Production Center of Transfusiology, Astana, Kazakhstan

Email: shenna1@yandex.ru

Head of Department of Laboratory Studies  of Transfusion Infections

Kazakhstan

A. A. Totolian

St. Petersburg Pasteur Institute, St. Petersburg, Russian Federation
Pavlov First Saint Petersburg State Medical University, St. Petersburg, Russian Federation

Email: shenna1@yandex.ru

RAS Full Member, PhD, MD (Medicine), Professor, Head of the Laboratory of Molecular Immunology, Director of St. Petersburg Pasteur Institute; Head of the Department of Immunology, Pavlov First St. Petersburg State Medical University, St. Petersburg, Russian Federation

Russian Federation

References

  1. Иванова Л.Ю. Информированность трудовых мигрантов из разных регионов об опасных инфекционных заболеваниях: ВИЧ, ИППП, туберкулез, гепатит (на материалах опроса иностранных работников в Санкт-Петербурге // Социальные аспекты здоровья населения. № 4. 2015. [Ivanova L.Yu. Awareness of labor migrants from different regions about dangerous infectious diseases: HIV, STIS, tuberculosis, hepatitis (based on survey of foreign workers in St. Petersburg). Sotsial’nye aspekty zdorov’ya naseleniya = Social Aspects of Public Health, no. 4, 2015. URL: http://vestnik.mednet.ru/content/view/702/30/lang,ru (In Russ.)]
  2. Останкова Ю.В., Семенов А.В., Файзуллаев Х.Н., Казакова Е.И., Козлов А.В., Мусабаев Э.И., Тотолян Арег А. Молекулярно-биологические маркеры гепатита В у пациентов с фиброзом/циррозом печени в Узбекистане // Журнал микробиологии, эпидемиологии и иммунобиологии. 2016. № 5. C. 34–43. [Ostankova Yu.V., Semenov A.V., Faizullaev H.N., Kazakova E.I., Kozlov A.V., Musabaev E.I., Totolian Areg A. Molecular biological markers of hepatitis B in patients with liver fibrosis/cirrhosis in Uzbekistan. Zhurnal mikrobiologii, epidemiologii i immunobiologii = Journal of Microbiology, Epidemiology and Immunobiology, 2016, no. 5, pp. 34–43. (In Russ.)]
  3. Семенов А.В., Останкова Ю.В., Герасимова В.В., Бичурина М.А., Мукомолов С.Л., Козлов А.В., Тотолян А.А. К вопросу о молекулярной эпидемиологии гепатита B в Республике Саха (Якутия) // Журнал инфектологии. 2016. Т. 8, № 1. С. 57–65. [Semenov A.V., Ostankova Yu.V., Gerasimova V.V., Bichurina M.A., Mukomolov S.L., Kozlov A.V., Totolian Areg A. To a question on the molecular epidemiology of hepatitis B in the Republic of Sakha (Yakutia). Zhurnal infektologii = Journal of Infectology, 2016, vol. 8, no. 1, pp. 57–65. (In Russ.)]
  4. Семенов А.В., Останкова Ю.В., Ногойбаева К.А., Касымбекова К.Т., Лаврентьева И.Н., Тобокалова С.Т., Тотолян Арег А. Особенности молекулярной эпидемиологии сочетанной инфекции ВГB/ВГD в Кыргызстане // Инфекция и иммунитет. 2016. Т. 6, № 2. С. 141–150. [Semenov A.V., Ostankova Yu.V., Nogoibaeva K.A., Kasymbekova K.T., Lavrenteva I.N., Tobokalova S.T., Totolian Areg A. Molecular epidemiology features of HBV/HDV co-infection in Kyrgyzstan. Infektsiya i immunitet = Russian Journal of Infection and Immunity, 2016. vol. 6, no. 2, pp. 141–150. doi: 10.15789/2220-7619-2016-2-141-150 (In Russ.)]
  5. Скорикова С.В., Буркитбаев Ж.К., Савчук Т.Н., Жибурт Е.Б. Распространенность ВИЧ-, ВГС-, ВГВ-инфекций у доноров крови Астаны // Вопросы вирусологии. 2015. Т. 60 (1). С. 34–36. [Skorikova S.V., Burkitbayev J.C., Savchuk T.N., Zhiburt E.B. The prevalence of HIV-, HCV-, HBV-infection in blood donors in Astana. Voprosy virusologii = Problems of Virology, 2015, vol. 60 (1), pp. 34–36. (In Russ.)]
  6. Avazova D., Kurbanov F., Tanaka Y., Sugiyama M., Radchenko I., Ruziev D., Musabaev E., Mizokami M. Hepatitis B virus transmission pattern and vaccination efficiency in Uzbekistan. J. Med. Virol., 2008, vol. 80, iss. 2, pp. 217–224. doi: 10.1002/jmv.21035
  7. Allain J.P. This article has been retracted: trends in the frequency of blood donors donating blood to be tested for HIV in Shiraz from 2004 to 2006. Transfus. Med., 2010, vol. 20, p. 127.
  8. Bertolini D.A., Gomes-Gouvêa M.S., Guedes de Carvalho-Mello I.M., Saraceni C.P., Sitnik R., Grazziotin F.G., Laurino J.P., Fagundes N.J., Carrilho F.J., Pinho J.R. Hepatitis B virus genotypes from European origin explains the high endemicity found in some areas from southern Brazil. Infect. Genet. Evol., 2012, vol. 12(6), pp. 1295–1304. doi: 10.1016/j.meegid.2012.04.009.
  9. Bissinger A.L., Fehrle C., Werner C.R., Lauer U.M., Malek N.P., Berg C.P. Epidemiology and genotyping of patients with chronic hepatitis B: genotype shifting observed in patients from Central Europe. Pol. J. Microbiol., 2015, vol. 64(1), pp. 15–21.
  10. Brichler S., Lagathu G., Chekaraou M.A., Le Gal F., Edouard A., Dény P., Césaire R., Gordien E. African, Amerindian and European hepatitis B virus strains circulate on the Caribbean Island of Martinique. J. Gen. Virol., 2013, vol. 94 (pt. 10), pp. 2318– 2329. doi: 10.1099/vir.0.055459-0
  11. Higgins D.G., Bleasby A.J., Fuchs R. CLUSTAL V: improved software for multiple sequence alignment. Comput. Appl. Biosci., 1992, vol. 8, pp. 189–191.
  12. Kasraian L., Tavasoli A. Positivity of HIV, hepatitis B and hepatitis C in patients enrolled in a confidential self-exclusion system of blood donation: a cross-sectional analytical study. Sao Paulo Med. J., 2010. vol. 128, pp. 320–323.
  13. Kramvis A., Paraskevis D. Subgenotype A1 of HBV-tracing human migrations in and out of Africa. Antivir. Ther., 2013. vol. 18 (3B), pp. 513–521. doi: 10.3851/IMP2657
  14. Lago B.V., Mello F.C., Kramvis A., Niel C., Gomes S.A. Hepatitis B virus subgenotype A1: evolutionary relationships between Brazilian, African and Asian isolates. PLoS One, 2014, vol. 9, iss. 8, e105317. doi: 10.1371/journal.pone.0105317
  15. Mokdad A.A., Lopez A.D., Shahraz S., Lozano R., Mokdad A.H., Stanaway J., Murray C.J., Naghavi M. Liver cirrhosis mortality in 187 countries between 1980 and 2010: a systematic analysis. BMC Med., 2014, vol. 12, p. 145. doi: 10.1186/s12916-014-0145-y
  16. Mössner B.K., Staugaard B., Jensen J., Lillevang S.T., Christensen P.B., Holm D.K. Dried blood spots, valid screening for viral hepatitis and human immunodeficiency virus in real-life. World J. Gastroenterol., 2016, vol. 22, iss. 33, pp. 7604–7612. doi: 10.3748/wjg.v22.i33.7604
  17. Rodríguez Lay L.A., Corredor M.B., Villalba M.C., Frómeta S.S., Wong M.S., Valdes L., Samada M., Sausy A., Hübschen J.M., Muller C.P. Genetic diversity of the hepatitis B virus strains in Cuba: absence of West-African genotypes despite the Transatlantic Slave Trade. PLoS One, 2015, vol. 10, iss. 5, e0125052. doi: 10.1371/journal.pone.0125052
  18. Saitou N., Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol., 1987, vol. 4, pp. 406–425.
  19. Stanojević B., Osiowy C., Schaefer S., Bojović K., Blagojević J., Nešić M., Yamashita S., Stamenković G. Molecular characterization and phylogenetic analysis of full-genome HBV subgenotype D3 sequences from Serbia. Infect. Genet. Evol., 2011, vol. 11, iss. 6, pp. 1475–1480. doi: 10.1016/j.meegid.2011.05.004
  20. Tallo T., Tefanova V., Priimagi L., Schmidt J., Katargina O., Michailov M., Mukomolov S., Magnius L., Norder H. D2: major sub-genotype of hepatitis B virus in Russia and the Baltic region. J. Gen. Virol., 2008, vol. 89, pp. 1829–1839. doi: 10.1099/vir.0.83660-0
  21. WHO Prevention and control of viral hepatitis infection: frame work for global action 2012. Geneva: WHO, 2012.
  22. Zehender G., Shkjezi R., Ebranati E., Gabanelli E., Abazaj Z., Tanzi E., Kraja D., Bino S., Ciccozzi M., Galli M. Reconstruction of the epidemic history of hepatitis B virus genotype D in Albania. Infect. Genet. Evol., 2012, vol. 12, pp. 291–298. doi: 10.1016/j.meegid.2011.11.009

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Copyright (c) 2017 Ostankova Y.V., Semenov A.V., Burkitbayev Z.K., Savchuk T.N., Totolian A.A.

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