VIRAL ETIOLOGY ACUTE INTESTINAL INFECTIONS MOLECULAR MONITORING IN CHILDREN’S HOSPITAL

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Abstract

On the territory of the Russian Federation in the overall structure of acute intestinal infections the proportion of viral diarrhea among children varies from 24 to 78% of cases depending on the season. The acute viral intestinal infections etiological confirmation is performed mainly among patients of infectious hospitals. The prevalence of viral acute intestinal infections in non-infectious hospitals, including infections associated with medical care, remains unclear. Currently estimation of viral component in the acute intestinal infections overall structure mainly consists in determination of rotavirus infection prevalence excluding other pathogens. As the part of viral etiology hospital infections epidemiological surveillance in non-infections children’s hospital the study of acute viral intestinal infections etiological structure and molecular genetics characterization of identified enteric viruses is conducted. The syndrome diagnosis of acute intestinal infections cases was introduced — an identification and evaluation of patients with signs of dysfunction of the gastrointestinal tract, that is not related to the underlying disease. A set of laboratory methods included identification of various intestinal pathogens DNA (RNA) by PCR-RT method; genotyping of enteric viruses using sequencing; nucleotide sequence analysis of cDNA fragments using the BLAST software package for identification of closely related strains and an online service for automatic genotyping of noroviruses by Norovirus Genotyping Tool Version 1.0. Alignment of nucleotide sequences and phylogenetic analysis was performed using the software MEGA 5.0. The obtained sequence fragments of the genome was downloaded in GenBank international database. The use of molecular genetics research methods allowed to differentiate viral pathogens of acute intestinal infections and to establish the fact of nosocomial transmission. The proportion of viral etiology acute intestinal infections in patients with clinical signs of intestinal infection, and contact persons was 43.8%. The etiological structure of intestinal virus infections was presented by noroviruses (73.2%) genotypes GII.1, GII.3, GII.4 Sydney 2012, rotaviruses (23.2%) genotypes G4P[8] and G1-IP[8], and adenovirus (1.8%) of the group F and astroviruses (1.8%) genotypes 1 and 2. Among the hospitalized children were 9 cases of viral etiology acute intestinal infections importation, and 66 of cases were of nosocomial origin. Examination of the environment revealed the presence of contamination by enteric viruses in 47.8% of cases. 

About the authors

A. V. Sergeeva

Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russian Federation

Author for correspondence.
Email: sergeeva-av2013@yandex.ru

PhD (Medicine), Senior Lecturer of the Department of Epidemiology, Head of Problematic Scientific Laboratory PCR-studies, Scientific Research Institute of Preventive Medicine, Nizhny Novgorod State Medical Academy of the Ministry of Public Health of the Russian Federation, Nizhny Novgorod, Russian Federation;

Россия

L. Y. Poslova

The State Budget Hospital Nizhny Novgorod Regional Children’s Clinical Hospital, Nizhny Novgorod, Russian Federation

Email: fake@neicon.ru

PhD (Medicine), Head of Epidemiological Department of the State Budget Hospital Nizhny Novgorod Regional Children’s Clinical Hospital, Nizhny Novgorod, Russian Federation; 

Россия

O. V. Kovalishena

Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russian Federation

Email: fake@neicon.ru

PhD, MD (Medicine), Professor of the Department of Epidemiology, Deputy Director of Science Scientific Research Institute of Preventive Medicine of the Nizhny Novgorod State Medical Academy of the Ministry of Public Health of the Russian Federation, Nizhny Novgorod, Russian Federation; 

Россия

A. S. Blagonravova

Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russian Federation

Email: fake@neicon.ru

PhD, MD (Medicine), Professor of the Department of Epidemiology, Director of Scientific Research Institute of Preventive Medicine of the Nizhny Novgorod State Medical Academy of the Ministry of Public Health of the Russian Federation, Nizhny Novgorod, Russian Federation;


Россия

N. V. Epifanova

Nizhny Novgorod Research Institute of Epidemiology and Microbiology named after Aсad. I.N. Blokhina, Nizhny Novgorod, Russian Federation

Email: fake@neicon.ru

PhD (Biology), Leading Researcher Assistant оf Laboratory of Molecular Epidemiology of Viral Infections, Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology named after Acad. I.N. Blokhina, Nizhny Novgorod, Russian Federation;


Россия

T. A. Sashina

Nizhny Novgorod Research Institute of Epidemiology and Microbiology named after Aсad. I.N. Blokhina, Nizhny Novgorod, Russian Federation

Email: fake@neicon.ru

Junior Researcher, Assistant оf Laboratory of Molecular Epidemiology of Viral Infections, Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology named after Acad. I.N. Blokhina, Nizhny Novgorod, Russian Federation;


Россия

O. V. Morozova

Nizhny Novgorod Research Institute of Epidemiology and Microbiology named after Aсad. I.N. Blokhina, Nizhny Novgorod, Russian Federation

Email: fake@neicon.ru

Junior Researcher, Assistant оf Laboratory of Molecular Epidemiology of Viral Infections, Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology named after Acad. I.N. Blokhina, Nizhny Novgorod, Russian Federation;

Россия

N. A. Novikova

Nizhny Novgorod Research Institute of Epidemiology and Microbiology named after Aсad. I.N. Blokhina, Nizhny Novgorod, Russian Federation

Email: fake@neicon.ru

PhD, MD (Biology), Professor, Head of Laboratory of Molecular Epidemiology of Viral Infections, Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology named after Acad. I.N. Blokhina, Nizhny Novgorod, Russian Federation. 

Россия

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Copyright (c) 2015 Sergeeva A.V., Poslova L.Y., Kovalishena O.V., Blagonravova A.S., Epifanova N.V., Sashina T.A., Morozova O.V., Novikova N.A.

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