THE EFFECT OF THE G141R EGG–ADAPTIVE SUBSTITUTION ON THE BIOLOGICAL PROPERTIES OF CURRENTLY CIRCULATING B/VICTORIA-LINEAGE INFLUENZA B VIRUSES



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Abstract

Abstract

Despite the continuing interest in influenza infection and relevant pathogens, a significant number of publications in this field are devoted to influenza A viruses, while influenza B viruses receive less attention. In PubMed database, there are 10 times fewer articles on influenza B vs. influenza A viruses. However, despite the complete disappearance of influenza B/Yamagata lineage viruses in the post-COVID-19 pandemic period, B/Victoria lineage viruses continue to circulate, posing a problem for national health. The fact that only B/Victoria lineage circulate currently in human population gives a reason to believe that it should be closely investigated. An important place among the major properties of influenza virus hemagglutinin (HA) is referred to its potential to shape antigenic diversity of influenza viruses. It has been reliably proven that mutations in specific positions of HA1 receptor-binding site affect antigenic specificity. Therefore, assessing key amino acid positions located in this area is of substantial scientific and practical interest. This is especially important in practice, e.g., in influenza vaccine production that includes passaging in a sensitive substrate (usually in developing chicken embryos) within manufacturing technology. During passaging, substrate-adapting substitutions may occur in the region of the receptor-binding pocket, which may lead to undesirable changes in antigenic and biological properties of vaccine preparation. Our study is aimed at assessing a role of one of such egg-adapting substitutions (G141R). It was found that passaging of two currently circulating influenza B/Victoria viruses, B/Austria/1359417/2021 and B/Catalonia/2279261NS/2023, and LAIV vaccine strains prepared on their platform in developing chicken embryos resulted in HA1 G141R substitution that increased cold-adaptation of LAIV candidates, but not affecting their antigenic properties, temperature-sensitive phenotype, or degree of HA thermostability. The data presented in the article indicate that it is necessary to pay close attention to the emergence of mutations in the genome of influenza vaccine strains during their preparation, monitoring their possible impact on the key properties of such strains.

About the authors

Ekaterina A. Stepanova

Institute of Experimental Medicine, Saint Petersburg, Russia

Email: fedorova.iem@gmail.com
ORCID iD: 0000-0002-8670-8645
SPIN-code: 8010-3047

PhD, Leading Researcher, Department of Virology and Immunology named after A.A. Smorodintsev

Россия, 12 Acad. Pavlov Street, 197022, St Petersburg, Russia

Ekaterina A. Bazhenova

Institute of Experimental Medicine, Saint Petersburg, Russia

Email: sonya.01.08@mail.ru
ORCID iD: 0000-0003-3280-556X
SPIN-code: 5169-1426

PhD, Senior Research Fellow, Department of Virology and Immunology named after A.A. Smorodintsev

Россия, 12 Acad. Pavlov Street, 197022, St Petersburg, Russia

Anna K. Chistyakova

Institute of Experimental Medicine, Saint Petersburg, Russia

Email: anna.k.chistiakova@gmail.com
ORCID iD: 0000-0001-9541-5636
SPIN-code: 8852-4103

Researcher, Department of Virology and Immunology named after A.A. Smorodintsev

Россия, 12 Acad. Pavlov Street, 197022, St Petersburg, Russia

Pei-Fong Wong

Institute of Experimental Medicine, Saint Petersburg, Russia

Email: po333222@gmail.com
ORCID iD: 0000-0002-7939-6313

Researcher, Department of Virology and Immunology named after A.A. Smorodintsev

Россия, 12 Acad. Pavlov Street, 197022, St Petersburg, Russia

Natalie V. Larionova

Institute of Experimental Medicine, Saint Petersburg, Russia

Email: nvlarionova@mail.ru
ORCID iD: 0000-0003-1171-3383
SPIN-code: 4709-5010

Ph.D., D.Sci., Leading Researcher, Department of Virology and Immunology named after A.A. Smorodintsev

Россия, 12 Acad. Pavlov Street, 197022, St Petersburg, Russia

Vera V. Kuzmicheva

Institute of Experimental Medicine, Saint Petersburg, Russia

Email: veralynx178@gmail.com

Department of Virology and Immunology named after A.A. Smorodintsev

Россия, 12 Acad. Pavlov Street, 197022, St Petersburg, Russia

Larisa G. Rudenko

Institute of Experimental Medicine, Saint Petersburg, Russia

Email: vaccine@mail.ru
ORCID iD: 0000-0002-0107-9959
SPIN-code: 4181-1372

Ph.D., D.Sci., Professor, Chief Researcher, Department of Virology and Immunology named after A.A. Smorodintsev

Россия, 12 Acad. Pavlov Street, 197022, St Petersburg, Russia

Irina V. Kiseleva

Institute of Experimental Medicine, Saint Petersburg, Russia

Author for correspondence.
Email: irina.v.kiseleva@mail.ru
ORCID iD: 0000-0002-3892-9873
SPIN-code: 7857-7306

Ph.D., D.Sci., Professor, Head of the Laboratory of the Department of Virology and Immunology named after A.A. Smorodintsev

Россия, 12 Acad. Pavlov Street, 197022, St Petersburg, Russia

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Copyright (c) Stepanova E.A., Bazhenova E.A., Chistyakova A.K., Wong P., Larionova N.V., Kuzmicheva V.V., Rudenko L.G., Kiseleva I.V.

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