LEPTOSPIROSIS IN THE REPUBLIC OF GUINEA
- Authors: Baimova R.R1, Karmokov I.A.1, Riabiko E.G.1, Khalilov E.S2, Blinova O.V.1, Tokarevich N.K.1, Boumbaly S.3, Camara A.3, Soropogui B.3, Camara J.3, Berete F.3
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Affiliations:
- St. Petersburg Pasteur Institute, St. Petersburg, Russian Federation
- Federal State Institution of Health "North-West Anti-Plague Station" of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, St. Petersburg, Russia
- Virology Research Center (CRV), Guinea Viral Hemorrhagic Fevers Laboratory. Conakry, Republic of Guinea
- Section: ORIGINAL ARTICLES
- Submitted: 29.05.2025
- Accepted: 23.09.2025
- URL: https://iimmun.ru/iimm/article/view/17937
- DOI: https://doi.org/10.15789/2220-7619-LIT-17937
- ID: 17937
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Abstract
Abstract
Leptospirosis is an acute zoonotic infection caused by pathogenic bacteria from the genus Leptospira. Leptospirosis creates significant though underdiagnosed public health problems in Africa. The Republic of Guinea (Guinea) is a country in West Africa, its capital is Conakry. The study objective was to assess the prevalence of the leptospirosis in febrile patients in the Republic of Guinea. We analyzed 827 blood serum samples obtained from febrile patients who applied to municipal health services, in the period from 2020 to 2024. RT-PCR was used to test 627 blood serum samples for the presence of genetic material of pathogenic Leptospira spp. Genotyping of positive samples was performed with the help of primers selected for a fragment of the secY gene. As a result of our study, IgG antibodies to Leptospira spp. were detected in 202 blood serum samples, which amounted to 24.4%.154 blood serum samples (18.6%) were positive for IgM to Leptospira spp. In 80 of the 276 positive samples both antibodies (IgG and IgM) were detected, 122 samples contained only IgG, while 74 only IgM. Leptospira spp. DNA was detected in 5 (0.8%) of 657 serum samples tested for the presence genetic markers of pathogenic leptospira. Sanger sequencing was applied to all 5 samples, and for three samples it resulted in successful leptospira genotyping, but it failed for two other samples may be due to insufficient amount of DNA or its degradation. When analyzed according to the BLAST algorithm, the obtained sequences revealed 100% similarity to those of L. kirschneri that was further confirmed by clustering when constructing the phylogenetic tree. Our study revealed high level of antibodies to leptospirosis in febrile patients, which bear witness to the wide spread of this pathogen in the Republic of Guinea. Joint research with international organizations can provide valuable information on the epidemiology of leptospirosis and improve control and prevention of the disease.
Keywords
About the authors
Regina R Baimova
St. Petersburg Pasteur Institute, St. Petersburg, Russian Federation
Email: baimova@pasteurorg.ru
ORCID iD: 0000-0002-0145-2653
Junior Researcher, Laboratory of Zooanthroponotic Infections
РоссияIslam A. Karmokov
St. Petersburg Pasteur Institute, St. Petersburg, Russian Federation
Email: karmokov2013@yandex.ru
ORCID iD: 0000-0003-3820-7106
SPIN-code: 8660-4404
Scopus Author ID: 57770227300
Junior Researcher, Laboratory of Zoonoses; Resident Physician, Department of Epidemiology, Parasitology and Disinfectology
Россия, St. Petersburg; St. PetersburgEkaterina G. Riabiko
St. Petersburg Pasteur Institute, St. Petersburg, Russian Federation
Email: katya.ryabiko@mail.ru
Junior Researcher, Laboratory of Zoonoses; Resident Physician, Department of Epidemiology, Parasitology and Disinfectology
Россия, St. Petersburg; St. PetersburgErik S Khalilov
Federal State Institution of Health "North-West Anti-Plague Station" of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, St. Petersburg, Russia
Email: erik.khalilov@yandex.ru
ORCID iD: 0000-0002-0599-4302
Biologist of the Virology Laboratory
Россия, Nevelskaya st., 3, St. Petersburg, 198035Olga V. Blinova
St. Petersburg Pasteur Institute, St. Petersburg, Russian Federation
Email: zoonoses@mail.ru
PhD (Chemistry), Junior Researcher, Laboratory of Zoonoses
Россия, St. PetersburgNikolay K. Tokarevich
St. Petersburg Pasteur Institute, St. Petersburg, Russian Federation
Email: zoonoses@mail.ru
DSc (Medicine), Professor, Head of the Laboratory of Zoonoses
Россия, St. PetersburgS. Boumbaly
Virology Research Center (CRV), Guinea Viral Hemorrhagic Fevers Laboratory. Conakry, Republic of Guinea
Email: drboumbaly@yahoo.fr
PhD (Microbiology), Director General
Гвинея, ConakryAlimou Camara
Virology Research Center (CRV), Guinea Viral Hemorrhagic Fevers Laboratory. Conakry, Republic of Guinea
Email: alimou.4camara@gmail.com
PhD (Immunology), Deputy Director General
Гвинея, ConakryBarré Soropogui
Virology Research Center (CRV), Guinea Viral Hemorrhagic Fevers Laboratory. Conakry, Republic of Guinea
Email: Barresoropogui@gmail.com
Head of the Molecular Biology Laboratory
Гвинея, ConakryJ. Camara
Virology Research Center (CRV), Guinea Viral Hemorrhagic Fevers Laboratory. Conakry, Republic of Guinea
Email: Jacob2240@gmail.com
Head of the Genomics Laboratory
Гвинея, ConakryF. Berete
Virology Research Center (CRV), Guinea Viral Hemorrhagic Fevers Laboratory. Conakry, Republic of Guinea
Author for correspondence.
Email: fantabere012015@gmail.com
Head of the Immunology Laboratory
Гвинея, ConakryReferences
- Баимова Р.Р., Рябико Е.Г., Останкова Ю.В., Токаревич Н.К. Оптимизация способа детекции и генотипирования патогенных лептоспир в биологических образцах. Клиническая лабораторная диагностика. 2025; 70 (3): 210-217 Baimova R.R., Ryabiko E.G., Ostankova Yu.V., Tokarevich N.K. (2025). Optimization of the method for detection and genotyping of pathogenic leptospires in biological samples. Clinical laboratory diagnostics (Russian journal) vol.70(3), pp.210-217 https://doi.org/10.51620/0869-2084-2025-70-3-210-217
- Allan, K. J., Biggs, H. M., Halliday, J. E., Kazwala, R. R., Maro, V. P., Cleaveland, S., & Crump, J. A. (2015). Epidemiology of Leptospirosis in Africa: A Systematic Review of a Neglected Zoonosis and a Paradigm for 'One Health' in Africa. PLoS neglected tropical diseases, 9(9), e0003899. https://doi.org/10.1371/journal.pntd.0003899
- Boland, M., Sayers, G., Coleman, T., Bergin, C., Sheehan, N., Creamer, E., O'Connell, M., Jones, L., & Zochowski, W. (2004). A cluster of leptospirosis cases in canoeists following a competition on the River Liffey. Epidemiology and infection, 132(2), 195–200. https://doi.org/10.1017/s0950268803001596
- de Vries, S. G., Visser, B. J., Stoney, R. J., Wagenaar, J. F. P., Bottieau, E., Chen, L. H., Wilder-Smith, A., Wilson, M., Rapp, C., Leder, K., Caumes, E., Schwartz, E., Hynes, N. A., Goorhuis, A., Esposito, D. H., Hamer, D. H., Grobusch, M. P., & For The GeoSentinel Surveillance Network (2018). Leptospirosis among Returned Travelers: A GeoSentinel Site Survey and Multicenter Analysis-1997-2016. The American journal of tropical medicine and hygiene, 99(1), 127–135. https://doi.org/10.4269/ajtmh.18-0020
- Dobigny, G., Garba, M., Tatard, C., Loiseau, A., Galan, M., Kadaouré, I., Rossi, J. P., Picardeau, M., & Bertherat, E. (2015). Urban Market Gardening and Rodent-Borne Pathogenic Leptospira in Arid Zones: A Case Study in Niamey, Niger. PLoS neglected tropical diseases, 9(10), e0004097.
- https://doi.org/10.1371/journal.pntd.0004097
- Fischer, S., Mayer-Scholl, A., Imholt, C., Spierling, N. G., Heuser, E., Schmidt, S., Reil, D., Rosenfeld, U. M., Jacob, J., Nöckler, K., & Ulrich, R. G. (2018). Leptospira Genomospecies and Sequence Type Prevalence in Small Mammal Populations in Germany. Vector borne and zoonotic diseases (Larchmont, N.Y.), 18(4), 188–199. https://doi.org/10.1089/vbz.2017.2140
- Franceschi, C., Salvioli, S., Garagnani, P., de Eguileor, M., Monti, D., & Capri, M. (2017). Immunobiography and the Heterogeneity of Immune Responses in the Elderly: A Focus on Inflammaging and Trained Immunity. Frontiers in immunology, 8, 982. https://doi.org/10.3389/fimmu.2017.00982
- Gizamba, J. M., & Mugisha, L. (2023). Leptospirosis in humans and selected animals in Sub-Saharan Africa, 2014-2022: a systematic review and meta-analysis. BMC infectious diseases, 23(1), 649. https://doi.org/10.1186/s12879-023-08574-5
- Gurman, T. A., Diallo, K., Larson, E., Sugg, K., & Tibbels, N. (2024). Balancing the uncertain and unpredictable nature of possible zoonotic disease transmission with the value placed on animals: Findings from a qualitative study in Guinea. PLOS global public health, 4(3), e0001174. https://doi.org/10.1371/journal.pgph.0001174
- Koffi, S. K., Meite, S., Ouattara, A., Kouassi, S. K., Aboubacar, S., Akran, V. A., Bourhy, P., & Dosso, M. (2018). Geographic distribution of anti-Leptospira antibodies in humans in Côte d'Ivoire, West Africa. European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 37(11), 2177–2180. https://doi.org/10.1007/s10096-018-3359-7
- Mane S. (2017). Revue des filières betail/viande & lait et des politiques qui les influences en Guinée. Organisation des Nations Unies pour l’alimentation et l’agriculture 2017. Mane S. (2017). Review of the livestock/meat & dairy sectors and the policies that influence them in Guinea. Food and Agriculture Organization of the United Nations, 2017. https://www.fao.org/3/i5268f/i5268f.pdf
- Mayer-Scholl, A., Teifke, J. P., Huber, N., Luge, E., Bier, N. S., Nöckler, K., & Ulrich, R. G. (2019). Leptospira spp. in Rodents and Shrews from Afghanistan. Journal of wildlife diseases, 55(2), 477–481. https://doi.org/10.7589/2018-05-122
- Oruganti, P., Root, E., Ndlovu, V., Mbhungele, P., Van Wyk, I., & Berrian, A. M. (2023). Gender and zoonotic pathogen exposure pathways in a resource-limited community, Mpumalanga, South Africa: A qualitative analysis. PLOS global public health, 3(6), e0001167. https://doi.org/10.1371/journal.pgph.0001167
- Palma, F. A. G., Costa, F., Lustosa, R., Mogaji, H. O., de Oliveira, D. S., Souza, F. N., Reis, M. G., Ko, A. I., Begon, M., & Khalil, H. (2022). Why is leptospirosis hard to avoid for the impoverished? Deconstructing leptospirosis transmission risk and the drivers of knowledge, attitudes, and practices in a disadvantaged community in Salvador, Brazil. PLOS global public health, 2(12), e0000408. https://doi.org/10.1371/journal.pgph.0000408
- Paquet, D., Jung, L., Trawinski, H., Wendt, S., & Lübbert, C. (2022). Fever in the Returning Traveler. Deutsches Arzteblatt international, 119(22), 400–407. https://doi.org/10.3238/arztebl.m2022.0182
- Roqueplo, C., Kodjo, A., Demoncheaux, J. P., Scandola, P., Bassene, H., Diatta, G., Sokhna, C., Raoult, D., Davoust, B., & Mediannikov, O. (2019). Leptospirosis, one neglected disease in rural Senegal. Veterinary medicine and science, 5(4), 536–544. https://doi.org/10.1002/vms3.186
- Soares, P. M., Gomes, D. O., Macedo, F. P., Soares, M. M., Lemes, K. R., Jaeger, L. H., Lilenbaum, W., & Lima, A. M. C. (2020). Serological and molecular characterization of Leptospira kirschneri serogroup Grippotyphosa isolated from bovine in Brazil. Microbial pathogenesis, 138, 103803. https://doi.org/10.1016/j.micpath.2019.103803
- World Health Organization 2025 data.who.int, Guinea [Country overview]. (Accessed on 8 May 2025) https://data.who.int/countries/324
- Yadeta, W., Bashahun, G.M., Abdela, N. (2016). Leptospirosis in animal and its public health implications: a review. World Applied Sciences Journal 34 (6): 845-853. https://doi.org/10.5829/idosi.wasj.2016.34.6.103113
- Zhang, Y., Ye, F., Xia, L. X., Zhu, L. W., Kamara, I. L., Huang, K. Q., Zhang, Y., Liu, J., Kargbo, B., Wang, J., Liang, M. F., Song, J. D., Ma, X. J., & Wu, G. Z. (2019). Next-generation Sequencing Study of Pathogens in Serum from Patients with Febrile Jaundice in Sierra Leone. Biomedical and environmental sciences : BES, 32(5), 363–370. https://doi.org/10.3967/bes2019.048
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