THE CIRCULATING IMMUNE COMPLEXES AS A BIOMARKER OF THE ENDOGENOUS INTOXICATION IN PULMONARY TUBERCULOSIS


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Abstract

Abstract. The aim of the study was to establish the concentration of the circulating immune complexes (CICs) with high (CIC-2,5%), medium (CIC-4,2%) and low (CIC-8.0%) molecular weights in patients with pulmonary tuberculosis (TB) as biomarkers of the endogenous intoxication and the degree of severity. We investigated 56 patients diagnosed with pulmonary TB and 36 healthy persons (control group). The patients were assigned to 2 groups: the 1st study group (1st SG) included 29 patients in which the test established the drug susceptible TB and in the 2nd study group (2ndSG) that included 27 patients with multidrug-resistant TB (MDR-TB). The inclusion criteria were: primary detected pulmonary TB, age older than 18 years, confirmed drug susceptibility (1st SG) and confirmed MDR-TB (2nd SG). The exclusion criteria were: anti-TB treatment in anamnesis, extrapulmonary TB, age below 18 years, HIV infection, severe somatic diseases. The method of CIC quantification consisted in placing 50 μl serum into 3 tubes followed by adding 100 μl borate buffer and solution of 2.5%, 4.2% and 8.0% polyethylene glycol. The tubes were incubated 15 minutes at 25oC and the optical density was assessed at the wavelength 340 nm. The results were compared with the general blood count and leucocital intoxication index Kalf-Kalif (LIIкк) as an alternative method to identify endogenous intoxication. As the results of the research we established that the peak level was observed for CICs with high and medium molecular weight, wrereas less pronounced it was for CICs with low molecular weight. The high severity of the endogenous intoxication predominated in the 1st SG and middle degree in the 2ndSG, without achieving the statistical significance. LII kk was lower in study groups comparing with the control healthy subjects due to increased count of eosinophils.

About the authors

E. V. Lesnic

Nicolae Testemitsanu State University of Medicine and Pharmacy, associated professor, Chisinau, Republic of Moldova

Author for correspondence.
Email: evelinalesnic@yahoo.com
ORCID iD: 0000-0002-4259-0227

PhD, Pneumophthisiology Department, NicolaeTestemitsanu State University of Medicine and Pharmacy, associated professor, Chisinau, Republic of Moldova

Moldova, Republic of

S. S. Ghinda

Nicolae Testemitsanu State University of Medicine and Pharmacy, associated professor, Chisinau, Republic of Moldova

Email: ginda-sergei@mail.ru
ORCID iD: 0000-0002-6458-4223

PhD, Immunology and Alergology Laboratory, Chiril Draganiuc Institute of Pneumophtysiology, Chisinau, Republic of Moldova, chief of laboratory.

Moldova, Republic of

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Copyright (c) 2022 Lesnic E.V., Ghinda S.S.

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