DETECTION AND PHYLOGENETIC ANALYSIS OF CLOSTRIDIUM VENTRICULI IN AUTISTIC CHILDREN



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Abstract

Abstract

Background: Autism spectrum disorder (ASD) is characterized by repetitive behaviors. There is evidence that gut flora imbalance may cause GI difficulties in autistic people. Gastrointestinal (GI) issues are associated with Clostridium ventriculi (C. ventriculi).

Aim: The purpose of this study was to use 16S rRNA gene sequencing to identify and genetically describe Clostridium ventriculi in fecal samples from children with autism.

Materials and Methods: A case-control study was done on fecal samples collected from 50 children diagnosed with autism. Also, samples were taken from 50 children who were not autistic as a control group. Using the FavorPrep Genomic DNA Mini Kit, DNA was extracted. PCR was used to amplify the 16S rRNA gene using the universal primers 27F and 1492R. After the PCR products were sequenced, BLAST and BioEdit tools were used to check the sequences for homology. The MEGA program was used for phylogenetic analysis.

Results: Based on PCR results, 10% (5/50) of the 50 samples of autistic children that were examined proved positive for C. ventriculi, and all control group were negative for this bacteria. Genetic polymorphisms were indicated by specific nucleotide transitions and transversions that were discovered by sequencing. The Iraqi isolates and global samples exhibited a high level of genetic similarity (99%) according to phylogenetic analysis, indicating a recent common ancestor and potential clonal expansion.

Conclusions: The discovery of C. ventriculi in autistic children raises the possibility of a connection between this bacteria and gastrointestinal problems linked to ASD.

About the authors

Alaa Hussein Hasan

Ibn Sina University of Medical and Pharmaceutical Sciences, Baghdad, Iraq

Email: alaa.hussein@ibnsina.edu.iq

Ph.D. Microbiology, Lecturer

Ирак

Husam Hussein Lazim

Ibn Sina University of Medical and Pharmaceutical Sciences, Baghdad, Iraq

Author for correspondence.
Email: hlazim@ibnsina.edu.iq

Ph.D. Microbiology, lecturer

Ирак

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Copyright (c) Hasan A.H., Lazim H.H.

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