Email this article SEROTONIN-RELATED IMMUNOMODULATORY EFFECT IN A MODEL OF A CONTAMINATED WOUND IN WARM-BLOODED ANIMALS
- Authors: Chibirova T.1,2, Skupnevskii S.1, Saveljev R.1, Kokaev R.2
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Affiliations:
- Federal State Budgetary Educational Institution of Higher Education "North Ossetian State University named after K.L. Khetagurov", Vladikavkaz, Russia
- Institute of Biomedical Investigations, Mikhaylovskoye village, Russia Resume
- Section: ORIGINAL ARTICLES
- Submitted: 24.02.2025
- Accepted: 09.06.2025
- URL: https://iimmun.ru/iimm/article/view/17863
- DOI: https://doi.org/10.15789/2220-7619-SRI-17863
- ID: 17863
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Abstract
Abstract
Introduction. The treatment of contaminated wounds remains a significant challenge, and the solution to this problem may lie in the realm of pathogenetic therapy.
The aim of this study was to investigate serotonin-related immunoregulatory effects in a contaminated wound model in experimental animals.
Materials and Methods. Wistar rats underwent surgical skin incisions in the epigastric region along the abdominal white line. Control animals received intraperitoneal saline (1st group) injections. Experimental animals were administered serotonin (days 0-5) at a dosage of 1.43 mg/kg b.w. intraperitoneally (2nd group); a serotonin pulse therapy (1,43; 1,43; 3,58; 7,15; 14,30 mg/kg) regimen was also administered (3rd group). After 5 days, the animals were euthanized, and microbiological, immunological, and histological analyses were performed.
Results. Upon serotonin treatment, total microbial wound and peri-wound contamination (both hemolytic and non-hemolytic) decreased by 27% compared to control group (p>0.05). The number of yeast-like and mold-like fungi also decreased significantly in experimental groups, by 4 times in the low dose group (1.43 mg/kg/day) and 4.6 times after pulse therapy. At the same time, an immunomodulatory effect was noted presented as increased neutrophil phagocytic activity, IL-6 levels, and activation of granulocyte lineage in the hematopoietic system. Histological analysis revealed accelerated skin regeneration in groups of treated animals.
Conclusion. Serotonin administration has an immunomodulatory effect in a model of contaminated wounds in animals, leading to reduced bacterial contamination and accelerated wound healing.
About the authors
Tamara Chibirova
Federal State Budgetary Educational Institution of Higher Education "North Ossetian State University named after K.L. Khetagurov", Vladikavkaz, Russia;Institute of Biomedical Investigations, Mikhaylovskoye village, Russia Resume
Email: tamaramerdenova@mail.ru
ORCID iD: 0000-0003-0819-8915
junior researcher at the Laboratory of Subcellular Cultures
Россия, 363110, РСО-Алания, с. Михайловское, ул. Вильямса д. 1Sergej Skupnevskii
Federal State Budgetary Educational Institution of Higher Education "North Ossetian State University named after K.L. Khetagurov", Vladikavkaz, Russia
Email: dreammas@yandex.com
ORCID iD: 0000-0002-6233-5944
Doctor of Biological Sciences, Head of the Laboratory of Systemic Environmental Analysis
Россия, Vatutina str., 44-46, Vladikavkaz, 362025, RussiaRodion Saveljev
Federal State Budgetary Educational Institution of Higher Education "North Ossetian State University named after K.L. Khetagurov", Vladikavkaz, Russia
Email: rodion.savelev23@gmail.com
ORCID iD: 0000-0002-4717-1734
laboratory assistant at the Laboratory of Systemic Environmental Analysis
Россия, Vatutina str., 44-46, Vladikavkaz, 362025, RussiaRomesh Kokaev
Institute of Biomedical Investigations, Mikhaylovskoye village, Russia Resume
Author for correspondence.
Email: romesh_k@mail.ru
ORCID iD: 0000-0002-2326-1348
Ph.D. of Medical Sciences, head of the Cell Technologies Laboratory
Россия, 363110, РСО-Алания, с. Михайловское, ул. Вильямса д. 1References
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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).