BIOLOGICAL PROPERTIES AND PATHOGENETIC POTENTIAL OF WEEKSELLACEAE FAMILY BACTERIA



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Abstract

In recent years, the role of former saprophyte bacteria in the etiology of human infectious diseases has become an increasingly pressing issue. Of particular interest are non-fermenting gram-negative bacteria, many of which are members of the rhizobiome in some plants, soil and water microbiome. Increasingly, scientific literature covers cases of infections caused by members of the order Flavobacteriales, namely the genus Chryseobacterium, Elizabethkingia and Empedobacter of the Weeksellaceae family. Microorganisms of the latter are causative agents in several human diseases, including urinary tract and respiratory tract infections. The aim of our study was to investigate the biological properties of bacteria of the genus Chryseobacterium, Elizabethkingia and Empedobacter of the Weeksellaceae family isolated from patients with cystic fibrosis and to assess their pathogenetic potential. To analyze the biological properties and pathogenetic potential, 32 clinical strains of bacteria belonging to the Weeksellaceae family, isolated from biological material samples of the respiratory tract from cystic fibrosis patients, were selected for the study. Differential diagnostic media and plate test systems were used. DNA was isolated using 5X ScreenMix (ZAO Evrogen) and PCR with gel electrophoresis was performed. Bacteria of the Weeksellaceae family are characterized by a wide range of enzymes, especially C. arthrosphaerae and E. meningoseptica. It was found that strains of the Chryseobacterium, Elizabethkingia and Empedobacter genus are carriers of virulence genes, exhibit pathogenicity and participate in chronic respiratory infections in patients with cystic fibrosis. Phenotypic methods for studying enzymatic activity showed that all the examined strains of bacteria of the Weeksellaceae family are characterized by gelatinase activity, and representatives of the genus Chryseobacterium spp. – also by the ability to synthesize plasma coagulase. The potential to produce other enzymes of aggression is less pronounced and was strain specific. Analysis of the genetic profile of virulence factors in the studied bacteria showed that in their genome, genes encoding the enzymes lecithinase, hyaluronidase, elastase and hemolysin were most often found, and in bacteria of the genus Chryseobacterium spp. and Empedobacter spp. – neuroaminidase, which contribute to overcoming tissue barriers, pathogen invasion and spread throughout host tissues. The obtained results and scientific literature data indicate a special clinical role for bacteria of the Weeksellaceae family, which determines the importance of a deep study of the biological properties and virulence factors in members of the genus Chryseobacterium spp., Elizabethkingia spp. and Empedobacter spp.

About the authors

Ksenia V. Zubova

Saratov State University

Email: zubovaksushechka@mail.ru

Assistant Professor, Department of Microbiology and Plant Physiology

Россия, 83 Astrakhanskaya Street, Saratov, Russia, 410012

Victoria A. Kuznetsova

"National Medical Research Center of Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov" of the Ministry of Health of the Russian Federation

Email: kuznecovaviktoria803@yandex.ru

 specialist of the Laboratory for the collection and storage of biomaterials

Россия, 4 Akademika Oparina street, Moscow, Russia, 117513

Matvey V. Kanevsky

Saratov State University

Email: matvejkanev@mail.ru

PhD, Associate Professor of the Department of Biochemistry and Biophysics, Head of the Educational and Scientific Laboratory of Molecular Biology

Россия, 83 Astrakhanskaya Street, Saratov, Russia, 410012

Olga V. Kondratenko

Federal State Budgetary Educational Institution of Higher Education Samara State Medical University of the Ministry of Health of the Russian Federation

Email: helga1983@yandex.ru

Doctor of Medical Sciences, Associate Professor, Acting Head of the Department of Medical Microbiology and Immunology

Россия, 89 Chapaevskaya Street, Samara, 443099, Russia

Elena V. Glinskaya

Saratov State University

Email: elenavg-2007@yandex.ru

PhD, Associate Professor, Department of Microbiology and Plant Physiology

Россия

Olga V. Nechaeva

"National Medical Research Center of Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov" of the Ministry of Health of the Russian Federation;
FSBEI FPE "Russian Medical Academy of Continuous Professional Education" of the Ministry of Healthcare of the Russian Federation

Email: olgav.nechaeva@rambler.ru
ORCID iD: 0000-0003-3331-1051
SPIN-code: 9984-9594

PhD, MD (Biology), Professor of the Department of Ecology and Technosphere Safety

Россия, 4 Akademika Oparina street, Moscow, Russia, 117513; 10/12 Polikarpova St., Moscow, 125284

Anna G. Afinogenova

St. Petersburg Pasteur Institut

Author for correspondence.
Email: spbtestcenter@mail.ru
ORCID iD: 0000-0001-8175-0708

doctor of biological science, head of testing laboratorial center

Россия

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Copyright (c) Zubova K.V., Kuznetsova V.A., Kanevsky M.V., Kondratenko O.V., Glinskaya E.V., Nechaeva O.V., Afinogenova A.G.

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