An accelerated method for determining «self/non-self» microorganisms in the agglutination reaction

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Abstract

A simple accelerated method for determining «self/non-self» microorganisms by using the agglutination reaction (RA) and therapeutic/prophylactic serum (Immunoglobulin complex preparation, lyophilized IgG, IgA, IgM immunoglobulins, developed by CSC Immuno-Gem, Moscow) is proposed to test for pathogenic, opportunistic and dominant probiotic Bifidobacteria spp. In parallel, all the microbial cell cultures examined were registered in the databases of Russia-wide and international collections and tested by the intermicrobial “self/non-self” recognition method, previously developed by us. 16 collection strains of various microorganisms were assessed by the RA with relevant therapeutic and prophylactic serum. Biological samples were obtained from the collection bacterial strains of Bifidobacterium bifidum 791, Escherichia coli LEGM-18, Klebsiella pneumoniae 278, Lactobacillus fermentum 90T-C4, Bifidobacterium longum MC-42, Escherichia coli M-17, Shigella sonnei 177b, Shigella flexneri 170, Escherichia coli 157, Staphylococcus aureus 209, Candida albicans 10231 and Salmonella serovar Enteritidis ATCC 10708. In addition, cell cultures obtained from the Museum of the Institute of Cellular and Intracellular Symbiosis UB RAS such as Bifidobacterium longum ICIS-505, Lactobacillus acidophilus ICIS-1127, Bifidobacterium bifidum ICIS-202, Bifidobacterium bifidum ICIS-310 were also included into the study. To assess microbial peptidoglycan foreignness, the intermicrobial “self/non-self” recognition method was also used based on inducing metabolites produced by the “dominant” test strain Bifidobacterium longum MC-42 after pre-incubation with metabolites collected from the studied cell cultures (“associates”) followed by established “dominant-associate” feedback loop. The data were evaluated by assessing change-fold in reproduction (growth/replication) and adaptation (biofilm formation and anti-lysozyme test) of microbial cultures in accordance with the described technique followed by comparing these two methods for intermicrobial “self/non-self” recognition. All the RA data were found to fully agree with those obtained after previous studies by using intermicrobial “self/non-self” recognition method coupled to “dominant-associate” system. Moreover, compared to analogous “intermicrobial recognition” method (5 days), ease of use and test timeframe (24 hours) allow to consider RA attractive for screening studies to select strains for scientific and industrial purposes.

About the authors

O. V. Bukharin

Institute of Cellular and Intracellular Symbiosis, Ural Branch of the Russian Academy of Sciences

Email: fake@neicon.ru
ORCID iD: 0000-0002-6039-5265
http://prof-ras.ru/index.php?option=com_k2&view=item&id=104-%D0%BF%D0%B5%D1%80%D1%83%D0%BD%D0%BE%D0%B2%D0%B0-%D0%BD%D0%B0%D1%82%D0%B0%D0%BB%D1%8C%D1%8F-%D0%B1%D0%BE%D1%80%D0%B8%D1%81%D0%BE%D0%B2%D0%BD%D0%B0

RAS Full Member, PhD, MD (Medicine), Head Researcher, Institute of Cellular and Intracellular Symbiosis.

Orenburg Russian Federation

N. B. Perunova

Institute of Cellular and Intracellular Symbiosis, Ural Branch of the Russian Academy of Sciences

Author for correspondence.
Email: perunovanb@gmail.com
ORCID iD: 0000-0002-6352-8879
http://prof-ras.ru/index.php?option=com_k2&view=item&id=104-%D0%BF%D0%B5%D1%80%D1%83%D0%BD%D0%BE%D0%B2%D0%B0-%D0%BD%D0%B0%D1%82%D0%B0%D0%BB%D1%8C%D1%8F-%D0%B1%D0%BE%D1%80%D0%B8%D1%81%D0%BE%D0%B2%D0%BD%D0%B0

Natalia B. Perunova - PhD, MD (Medicine), Professor of RAS, Leading Researcher (with the Duties of the Head of the Laboratory), Biomonitoring and Molecular Genetic Research Laboratory, Institute of Cellular and Intracellular Symbiosis.

460000, Orenburg, Pionerskaya str., 11, Phone: +7 922 555-30-80

Russian Federation

I. N. Chainikova

Institute of Cellular and Intracellular Symbiosis, Ural Branch of the Russian Academy of Sciences

Email: inchainicova@yandex.ru
ORCID iD: 0000-0002-8923-8829

PhD, MD (Medicine), Professor, Leading Researcher, Biomonitoring and Molecular Genetic Research Laboratory, Institute of Cellular and Intracellular Symbiosis.

Orenburg

Russian Federation

E. V. Ivanova

Institute of Cellular and Intracellular Symbiosis, Ural Branch of the Russian Academy of Sciences

Email: walerewna13@gmail.com
ORCID iD: 0000-0002-4974-8947

Associate Professor, Leading Researcher, Biomonitoring and Molecular Genetic Research Laboratory, Institute of Cellular and Intracellular Symbiosis.

Orenburg

Russian Federation

S. V. Andryushchenko

Institute of Cellular and Intracellular Symbiosis, Ural Branch of the Russian Academy of Sciences

Email: rattus000@gmail.com
ORCID iD: 0000-0003-1691-7588

PhD (Medicine), Senior Researcher, Biomonitoring and Molecular Genetic Research Laboratory, Institute of Cellular and Intracellular Symbiosis.

Orenburg

Russian Federation

References

  1. Андрющенко С.В., Перунова Н.Б., Бухарин О.В. Молекулярные механизмы взаимодействия бактерий с лизоцимом и их роль в микросимбиоценозе // Успехи современной биологии. 2015. Т. 135, № 5. С. 453—463.
  2. Бухарин О.В. Инфекционная симбиология — новое понимание старых проблем // Вестник РАН. 2016. Т. 86, № 10. С. 915—920. doi: 10.7868/S0869587316070033 (In Russ.)
  3. Бухарин О.В. Персистенция патогенных бактерий. М.: Медицина, 1999. 365 с.
  4. Бухарин О.В., Перунова Н.Б. Феномен микробного распознавания как медико-биологическая проблема. В кн.: Микросимбиоценоз. Екатеринбург: УрО РАН, 2014. С. 181—217.
  5. Andryuschenko S.V., Ivanova E.V., Perunova N.B., Bukharin O.V. Genome sequence and biochemical properties of Bifidobacterium longum strain ICIS-505, isolated from the intestine of a healthy woman. Microbiol. Recour. Announc., 2019, vol. 8, no. 33, pp. e00491—19. doi: 10.1128/MRA.00491-19
  6. Andryuschenko S.V., Ivanova E.V., Perunova N.B., Zdvizhkova I.A., Bekpergenova A.V., Bukharin O.V. Draft genome sequence of Bifidobacterium bifidum strain ICIS 310 Isolated from а feces of a healthy 5-year-old child from Orenburg. Microbiol. Recour. Announc., 2018, vol. 7, no. 18, pp. e01271—18. doi: 10.1128/MRA.01271—18
  7. Andryuschenko S.V., Zdvizhkova I.A., Perunova N.B., Bukharin O.V. Draft genome sequence of Klebsiella pneumoniae strain ICIS-278_PBV, isolated from the feces of a healthy 59-year-old man from Orenburg, Russia. Genome Announc., 2018, vol. 6, no. 27, pp. e00576—18. doi: 10.1128/genomeA.00576-18.
  8. Bukharin O.V. Adaptive strategies of pathogen-host interrelationships under infection. Her. Russ. Acad. Sci, 2018, vol. 88, no. 4, pp. 294 -299. doi: 10.1134/S1019331618040020
  9. Lilja E.E., Johnson D.R. Segregating metabolic processes into different microbial cells accelerates the consumption of inhibitory substrates. ISME J., 2016, vol. 10, no. 7, pp. 1568-1578. doi: 10.1038/ismej.2015.243
  10. Sakurai T., Hashikura N., Minami J., Yamada A., Odamaki T., Xiao J.Z. Tolerance mechanisms of human-residential bifidobac-terial against lysozyme. Anaerobe, 2017, no. 47, pp. 104-110. doi: 10.1016/j.anaerobe.2017.05.001

Copyright (c) 2020 Bukharin O.V., Perunova N.B., Chainikova I.N., Ivanova E.V., Andryushchenko S.V.

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