Email this article MULTIDRUG-RESISTANCE AND PRESENCE OF CLASS 1 INTEGRONS IN CLINICAL ISOLATES OF SALMONELLA ENTERICA SEROTYPE ENTERITIDIS, CIRCULATING IN ARMENIA
- Authors: Sedrakyan A.M.1, Arakelova K.A.1, Zakaryan M.K.1, Hovhanisyan A.I.1, Asoyan A.V.2, Gevorkyan Z.U.2, Mnatsakanyan A.A.2, Ktsoyan Z.A.1, Boyajyan A.S.1, Aminov R.3
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Affiliations:
- Institute of Molecular Biology of National Academy of Sciences of the Republic of Armenia
- “Nork” Clinical Hospital of Infectious Diseases, Ministry of Health of the Republic of Armenia, Yerevan
- National Veterinary Institute, Technical University of Denmark, Copenhagen
- Issue: Vol 3, No 4 (2013)
- Pages: 355-358
- Section: SHORT COMMUNICATIONS
- Submitted: 08.07.2014
- Accepted: 08.07.2014
- Published: 08.07.2014
- URL: https://iimmun.ru/iimm/article/view/145
- DOI: https://doi.org/10.15789/2220-7619-2013-4-355-358
- ID: 145
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Abstract
Abstract. The aim of this work was detection of class 1 integrons and their contribution to the antimicrobial resistance phenotypes in strains of subspecies enterica serotype Enteritidis. S. Enteritidis strains (n = 29) were isolated from patients with salmonellosis at “Nork” Clinical Hospital of Infectious Diseases, Yerevan, Republic of Armenia. High prevalence of multi-drug resistance (MDR) phenotypes was revealed and isolates with MDR phenotypes which are rare in the S. Enteritidis serotype were observed. Class 1 integrons were detected in 27,6% of isolates, with the prevalence of a variable region of 1000 bp. Occurrence of the MDR phenotype was more frequent in integron-positive isolates compared to integron-negative isolates of S. Enteritidis. Further studies are necessary to reveal the genetic background of MDR phenotypes and to estimate the genetic kinship among the isolates. Our results suggest a rapid and large-scale penetration of antibiotic resistance genes into populations of S. Enteritidis, which complicates infection control. More rigorous regulations should be imposed on antibiotic use, together with a vigilant epidemiological surveillance, to prevent the emergence and spread of MDR S. Enteritidis.
About the authors
A. M. Sedrakyan
Institute of Molecular Biology of National Academy of Sciences of the Republic of Armenia
Author for correspondence.
Email: sedanahit@rambler.ru
PhD (Biology), Senior Researcher, Laboratory of Molecular Genetics
0014, Republic of Armenia, Yerevan, Asratyan str., 7
АрменияK. A. Arakelova
Institute of Molecular Biology of National Academy of Sciences of the Republic of Armenia
Email: sedanahit@rambler.ru
Master’s degree, Researcher, Laboratory of Molecular Genetics Армения
M. K. Zakaryan
Institute of Molecular Biology of National Academy of Sciences of the Republic of Armenia
Email: sedanahit@rambler.ru
Master’s degree, Junior Researcher, Laboratory of Molecular Genetics Армения
A. I. Hovhanisyan
Institute of Molecular Biology of National Academy of Sciences of the Republic of Armenia
Email: sedanahit@rambler.ru
PhD (Biology), Researcher, Laboratory of Molecular Genetics Армения
A. V. Asoyan
“Nork” Clinical Hospital of Infectious Diseases, Ministry of Health of the Republic of Armenia, Yerevan
Email: sedanahit@rambler.ru
PhD (Medicine), Associate Professor, Director Армения
Z. U. Gevorkyan
“Nork” Clinical Hospital of Infectious Diseases, Ministry of Health of the Republic of Armenia, Yerevan
Email: sedanahit@rambler.ru
PhD (Medicine), Head of the Laboratory Services Армения
A. A. Mnatsakanyan
“Nork” Clinical Hospital of Infectious Diseases, Ministry of Health of the Republic of Armenia, Yerevan
Email: sedanahit@rambler.ru
Bacteriologist Армения
Z. A. Ktsoyan
Institute of Molecular Biology of National Academy of Sciences of the Republic of Armenia
Email: sedanahit@rambler.ru
PhD (Biology), Leading Researcher, Head of the Laboratory of Molecular Genetics Армения
A. S. Boyajyan
Institute of Molecular Biology of National Academy of Sciences of the Republic of Armenia
Email: sedanahit@rambler.ru
PhD, MD (Biology), Professor, Director Армения
R. Aminov
National Veterinary Institute, Technical University of Denmark, Copenhagen
Email: sedanahit@rambler.ru
PhD (Biology), Senior Researcher Дания
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