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Abstract. Pathogenesis of chronic hepatitis C (CHC) remains to be determined. Mechanisms of liver parenchyma damage in patients with CHC are complex and different. Cytokines play the role of intermediaries in the process of fibrosis development and chronic inflammation. In the present study levels of 27 cytokines in the blood plasma of 14 patients with CHC were tested using multiplex analysis. The liver biopsy was performed in all patients to define the activity of inflammation (histological activity index) and the degree of fibrosis. Nineteen samples of blood plasma obtained from healthy individuals were served as a control group in this study. The following cytokines were measured: IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17, eotaxin, FGF-2, G-CSF, GM-CSF, IFNγ, IP-10, MCP-1, MIP-1α, MIP-1β, RANTES, PDGF-BB, TNFα and VEGF. In patients with CHC elevated levels of plasma IL-1ra, IL-6, IL-7, IFNγ, IL-12 (p70), IL-4, IL-9, IL-8, IP-10, eotaxin, MCP-1, MIP-1β, TNFα, G-CSF and GM-CSF were found in compare with the control group. At the same time levels of FGF-2 and PDGF-BB were reduced in patients with CHC in compare with controls. Differences in the production of IL-1ra, IL-6, IL-7, IFNγ, IL-12 (p70), IL-4, IL-9, IL-8, IP-10, eotaxin, MCP-1, MIP-1β, TNFα, G-CSF and GM-CSF were depend on the genotype of HCV (3a or 1b), histological activity index in liver tissue and the degree of liver fibrosis. The revealed changes of cytokine production in patients with CHC characterize different orientation of regulatory violations confirming that CHC is an immunopathological process.

About the authors

K. A. Sysoev

Санкт-Петербургский государственный медицинский университет им. акад. И.П. Павлова, Санкт-Петербург

Author for correspondence.

к.м.н., старший научный сотрудник НМЦ по молекулярной медицине МЗ РФ

197022, Санкт-Петербург, ул. Льва Толстого, 6/8

Russian Federation

A. V. Chukhlovin

Санкт-Петербургский государственный медицинский университет им. акад. И.П. Павлова, Санкт-Петербург

Russian Federation

D. M. Shakhmanov

Военно-медицинская академия им. С.М. Кирова, Санкт-Петербург

Russian Federation

K. V. Zhdanov

Военно-медицинская академия им. С.М. Кирова, Санкт-Петербург

Russian Federation

Areg A. Totolian

ФБУН НИИ эпидемиологии и микробиологии имени Пастера, Санкт-Петербург

Russian Federation


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Copyright (c) 2014 Sysoev K.A., Chukhlovin A.V., Shakhmanov D.M., Zhdanov K.V., Totolian A.A.

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