Comparison of the percentage of NRBC/100 WBC in early onset sepsis and non-infectious premature infants

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Introduction. In spite of significant advances in medical care, neonatal sepsis remains an important risk factor for neonatal morbidity and mortality. Accordingly, the present study was conducted to compare the number of nucleated red blood cells per 100 white blood cells (NRBC/100WBC) in neonates with early onset sepsis and non-infectious neonates. Materials and methods. In this cross-sectional study of 154 neonates admitted to the NICU of Ghaem Hospital in Mashhad, Iran within the first three days of life, during 2014 to 2018, the characteristics of 44 neonates identified early onset sepsis (Case group) were compared with 110 non-infectious neonates (Control group). After the confirmation of sepsis in neonates based on positive blood culture and laboratory results, a researcher-made questionnaire containing neonatal characteristics (gestational age, weight, first minute Apgar scores, fifth minute Apgar score, duration of oxygen therapy, and mechanical ventilation duration) and neonatal laboratory profiles (routine blood culture, WBC, NRBC/100WBC, CRP, blood glucose, calcium and venous blood gas) was filled in. Results. The results of this study showed that the absolute number of NRBC/mm3 in control group was 56.07±86.65 and in case group was 592.70±1166.75 (p = 0.000). Also, the number of NRBC per 100 white blood cells in control group was 6.54±11.18 and in case group was 31.84±40.07 (p = 0.000). The absolute number of NRBC/mm3 for the detection of early onset sepsis had a good sensitivity (78%) and NRBC/100WBC was suitable specificity (68.2%). Conclusion. This study indicated that NRBC/100 WBC and absolute NRBC count/mm3 can be helpful in the diagnosis of early onset sepsis and have an acceptable sensitivity and specificity.

About the authors

H. Boskabadi

Mashhad University of Medical Sciences

Email: boskabadih@mums.ac.ir

Hassan Boskabadi - Professor, Department of Pediatrics, Faculty of Medicine, Mashhad University of Medical Sciences.

Mashhad.

Iran, Islamic Republic of

M. H. Sadeghian

Mashhad University of Medical Sciences

Email: sadeghianmh@mums.ac.ir

Mohammad Hadi Sadeghian - Associate Professor, Department of Hematopathology, Ghaem Hospital, Mashhad University of Medical Sciences.

Mashhad.

Iran, Islamic Republic of

B. Meshkani

Mashhad University of Medical Sciences

Email: meshkaniba@mums.ac.ir

Baratali Mashkani - Associate Professor, Department of Medical Biochemistry, School of Medicine, Mashhad University of Medical Sciences.

Mashhad.

Iran, Islamic Republic of

M. Zakerihamidi

Islamic Azad University, Tonekabon Branch

Author for correspondence.
Email: maryamzakerihamidi@yahoo.co.nz

Maryam Zakerihamidi - PhD (Reproductive Health), Assistant Professor of Reproductive Health, Department of Midwifery, Faculty of Medical Sciences, Islamic Azad University, Tonekabon Branch.

Tonekabon.

Phone: +98 0115 427-11-05

Iran, Islamic Republic of

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