Genetic diversity of hepatitis C virus in Nanaian region, Khabarovsk territory

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Abstract

Examining hepatitis C virus (HCV) genetic diversity is of great practical value in molecular-epidemiological research, development of specific prevention tools and outlining therapeutic strategy.

Aim of study is to conduct analysis assessing HCV genetic diversity circulating in the Nanaisky Region population of the Khabarovsk Krai.

Materials and methods. Molecular and genetic analysis of 124 blood plasma samples collected from patients with chronic hepatitis C and residing in the Nanaisky Region was conducted.

Results. HCV RNA was detected in 84 (67.7±4.2%) plasma samples. HCV genotyping was performed by using AmpliSens — 1/2/3 kit (Central Research Institute of Epidemiology, Moscow, Russian Federation) showing that genotype 3 dominated reaching up to 47.6±5.4% (n = 40). Genotype 1 was detected in 30 patients (35.7±5.2%). In thirteen cases (15.5±3.9%) genotype 2 was identified, whereas in one case (1.2±1.2%) virus genotype was unidentified. Phylogenetic analysis of the nucleotide sequences in HCV NS5B region was performed for 60 HCV RNA-positive samples showing subtype ratio as follows: 1a — 2 (3.3±2.3%), 1b — 23 (38.3±6.3%), 2а — 6 (10.0±3.9%), 2с — 2 (3.3±2.3%), 3а — 27 (45.0±6.4%). Three samples of RF2k/1b recombinant virus were found. A full NS2 gene nucleotide sequence was cloned in order to confirm the recombination event. The results of the study evidence about a need to conduct multi-layered examination of patients with chronic hepatitis C by using current molecular and biologic methods for assigning proper therapy coupled to characteristics of the isolated strains. The data regarding hepatitis C virus molecular and genetic parameters circulating in the Far Eastern Federal District, Russia, are rather limited. Hence, our study would contribute to current understanding of HCV genovariants circulating in territories of the Russian Federation.

About the authors

V. O. Kotova

Khabarovsk Research Institute of Epidemiology and Microbiology of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Shevchenko str., 2, Khabarovsk, 680610,Russian Federation

Author for correspondence.
Email: kotova.valeriya@mail.ru

Valeriia O. Kotova - Senior Researcher, Head of the Laboratory of Epidemiology and Prevention of Viral Hepatitis and AIDS.

680000, Khabarovsk, Shevchenko str., 2, Phone: +7 (4212) 46-18-54

Russian Federation

L. A. Balakhontseva

Khabarovsk Research Institute of Epidemiology and Microbiology of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Shevchenko str., 2, Khabarovsk, 680610,Russian Federation

Email: kotova.valeriya@mail.ru

Head of the Far Eastern Regional Center on Prevention and Combat Against AIDS.

Khabarovsk

Russian Federation

E. A. Bazykina

Khabarovsk Research Institute of Epidemiology and Microbiology of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Shevchenko str., 2, Khabarovsk, 680610,Russian Federation

Email: kotova.valeriya@mail.ru

Junior Researcher, Laboratory of Epidemiology and Prevention of Viral Hepatitis and AIDS.

Khabarovsk

Russian Federation

O. E. Trotsenko

Khabarovsk Research Institute of Epidemiology and Microbiology of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Shevchenko str., 2, Khabarovsk, 680610,Russian Federation

Email: kotova.valeriya@mail.ru

PhD, MD (Medicine), Head.

Khabarovsk

Russian Federation

V. N. Beldy

Government-owned publicly funded health care institution “Troitsk central regional hospital” of the Ministry of Heathcare of the Khabarovsk krai, Pushnikova str., 10, Troitskoe, Nanaisky region of the Khabarovsk krai, 682350, Russian Federation

Email: kotova.valeriya@mail.ru

Infectologist

Troitskoe, Nanaisky region of the Khabarovsk krai

Russian Federation

S. E. Kirdyashova

Government-owned publicly funded health care institution “Troitsk central regional hospital” of the Ministry of Heathcare of the Khabarovsk krai, Pushnikova str., 10, Troitskoe, Nanaisky region of the Khabarovsk krai, 682350, Russian Federation

Email: kotova.valeriya@mail.ru

Head Physician.

Troitskoe, Nanaisky region of the Khabarovsk krai

Russian Federation

References

  1. Дементьева Н.Е., Калинина О.В., Знойко О.О., Беляков Н.А., Жебрун А.Б. Циркулирующая рекомбинантная форма вируса гепатита С RF2k/1b: проблемы диагностики и терапии // ВИЧ-инфекция и иммуносупрессии. 2016. Т. 8, № 1. С. 42—52. doi: 10.22328/2077-9828-2016-8-1-42-52
  2. Кравченко А.В., Куимова У.А., Ганкина Н.Ю., Канестри В.Г., Чуланов В.П. Предикторы устойчивого вирусологического ответа при терапии хронического гепатита пегилированным интерфероном и рибавирином у больных ВИЧ-инфекцией // Инфекционные болезни. 2014. Т. 12, № 2. С. 30—34.
  3. Кюрегян К.К., Михайлов М.И. Молекулярно-биологические основы контроля вирусных гепатитов. М.: Икар, 2013. 336 с.
  4. Львов Д.К., Самохвалов Е.И., Миширо С., Тсуда Ф., Селиванов Н.А., Окамото Х., Стаханова В.М. Закономерности распространения вируса гепатита С и его генотипов в России и странах СНГ // Вопросы вирусологии. 1997. № 4. С. 157—161.
  5. Мукомолов С.Л., Левакова Л.Г., Сулягина Е.В., Синайская Е.В., Болсун Д.Д., Иванова Н.В. Современная эпидемиология гепатита С в России // Эпидемиология и инфекционные болезни. Актуальные вопросы. 2012. № 6. C. 21—25.
  6. Семенов А.В., Останкова Ю.В., Герасимова В.В., Бичурина М.А., Козлов А.В., Мукомолов С.Л., Тотолян А.А. Молекулярно-эпидемиологические особенности изолятов вируса гепатита С из разных регионов Республики Саха (Якутия) // Инфекция и иммунитет. 2015. Т. 5, № 4. С. 359—372. doi: 10.15789/2220-7619-2015-4-359-372
  7. Соболева Н.В., Карлсен А.А., Кожанова Т.В., Кичатова В.С., Клушкина В.В., Исаева О.В., Игнатьева М.Е., Романенко В.В., Ооржак Н.Д., Малинникова Е.Ю., Кюрегян К.К., Михайлов М.И. Распространенность вируса гепатита С среди условно здорового населения Российской Федерации // Журнал инфектологии. 2017. Т. 9, № 2. С. 56—64.
  8. Чуб Е.В., Кочнева Г.В., Гранитов В.М., Нетесов С.В. Рекомбинанты вируса гепатита С типа 2k/1bY населения Алтайского края // Инфекционные болезни. 2007. Т. 5, № 4. С. 5—11. [Chub E.V., Kochneva G.V., Granitov V.M., Netesov S.V. Recombinants of hepatitis С virus type 2k/1b in the population of the Altai region. Infektsionnye bolezni = Infectious Diseases, 2007, vol. 5, no. 4, pp. 5-11. (In Russ.)]
  9. Чуланов В.П., Пименов Н.Н., Мамонова Н.А., Сагалова О.И., Шестакова И.В., Покровский В.И. Хронический гепатит С как проблема здравоохранения России сегодня и завтра // Терапевтический архив. 2015. № 11. С. 5—10.
  10. Borgia S.M., Hedskog C., Parhy B., Hyland R.H., Stamm L.M., Brainard D.M., Subramanian M.G., McHutchison J.G., Mo H., Svarovskaia E., Shafran S.D. Identification of a novel hepatitis C virus genotype from Punjab, India: expanding classification of hepatitis C virus into 8 genotypes. J. Infect. Dis., 2018, vol. 218, no. 11, pp. 1722-1729. doi: 10.1093/infdis/jiy401
  11. Bukh J. The history of hepatitis C virus (HCV): Basic research reveals unique features in phylogeny, evolution and the viral life cycle with new perspectives for epidemic control. J. Hepatol., 2016, vol. 65, no. 1, pp. S2-S21. doi: 10.1016/j.jhep.2016.07.035
  12. Demetriou V.L., Kyriakou E., Kostrikis L.G. Near-full genome characterisation of two natural intergenotypic 2k/1b recombinant hepatitis C virus isolates. Adv. Virol., 2011: 710438, 7p. doi: 10.1155/2011/710438
  13. International Committee on Taxonomy of Viruses (ICTV), 2019. URL: https://talk.ictvonline.org (30.05.2019)
  14. Kalinina O., Norder H., Mukomolov S., Magnius L.O. A natural intergenotypic recombinant of hepatitis C virus identified in St. Petersburg. J. Virol., 2002, vol. 76, no. 8, pp. 4034-4043. doi: 10.1128/jvi.76.8.4034-4043.2002
  15. Kalinina O., Norder H., Magnius L.O. Full-length open reading frame of a recombinant hepatitis C virus strain from St. Petersburg: proposed mechanism for its formation. J. Gen. Virol., 2004, vol. 85, no. 7,pp. 1853—1857. doi: 10.1099/vir.0.79984-0
  16. Kuiken C., Simmonds P. Nomenclature and numbering of the hepatitis C virus. Methods Mol. Biol., 2009, no. 510, pp. 33—53. doi: 10.1007/978-1-59745-394-3_4
  17. Messina J., Humphreys I., Flaxman A., Brown A., Cooke G.S., Pybus O.G., Barnes E. Global distribution and prevalence of hepatitis C virus genotypes. Hepatology, 2015, vol. 61, no. 1, pp. 77—87. doi: 10.1002/hep.27259
  18. Morel V., Descamps V., Francois C., Fournier C., Brochot E., Capron D., Duverlie G., Castelain S. Emergence of a genomic variant of the recombinant 2k/1b strain during a mixed hepatitis C infection: a case report. J. Clin. Virol., 2010, vol. 47, no. 4, pp. 382-386. doi: 10.1016/j.jcv.2010.01.011
  19. Rajhi M., Ghedira K., Chouikha A., Djebbi A., Cheikh I., Ben Yahia A., Sadraoui A., Hammami W., Azouz M., Ben Mami N., Triki H. Phylogenetic analysis and epidemic history of hepatitis C virus genotype 2 in Tunisia, North Africa. PLoS One, 2016, vol. 11, no. 4: e0153761. doi: 10.1371/journal.pone.0153761
  20. Simmonds P. Genetic diversity and evolution of hepatitis C virus-15 years on. J. Gen. Virol., 2004, vol. 85, no. 11, pp. 3173-3188. doi: 10.1099/vir.0.80401-0
  21. Simmonds P., Bukh J., Combet C., Deleage G., Enomoto N., Feinstone S., Halfon P., Inchauspe G., Kuiken C., Maertens G., Mizokami M., Murphy D.G., Okamoto H., Pawlotsky J.M., Penin F., Sablon E., Shin-I T., Stuyver L.J., Thiel H.J., Viazov S., Weiner A.J., Widell A. Consensus proposals for a unified system of nomenclature of hepatitis C virus genotypes. Hepatology, 2005, vol. 42, no. 4, pp. 962-973. doi: 10.1002/hep.20819
  22. Smith D.B., Bukh J., Kuiken C., Muerhoff A.S., Rice C.M., Stapleton J.T., Simmonds P. Expanded classification of hepatitis C virus into 7 genotypes and 67 subtypes: updated criteria and genotype assignment web resource. Hepatology, 2014, vol. 59, no. 1, pp. 318-327. doi: 10.1002/hep.26744
  23. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol., 2013, vol. 30, no. 12, pp. 2725-2729. doi: 10.1093/molbev/mst197
  24. Thong V.D., Akkarathamrongsin S., Poovorawan K. Hepatitis C virus genotype 6: virology, epidemiology, genetic variation and clinical implication. World J. Gastroenterol., 2014, vol. 20, no. 11, pp. 2927-2940. doi: 10.3748/wjg.v20.i11.2927
  25. WHO. Global hepatitis report, 2017. Geneva: WHO, 2017. 83 p. URL: https://www.who.int/hepatitis/publications/global-hepatitis-report2017/en (15.05.2019)

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Copyright (c) 2021 Kotova V.O., Balakhontseva L.A., Bazykina E.A., Trotsenko O.E., Beldy V.N., Kirdyashova S.E.

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