State of rat colon microbiocenosis in chronic restraint stress treated with Selank

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Abstract

It is currently accepted that stress significantly affects composition of microbiocenosis due to changes in permeability of intestinal barrier and pro-inflammatory effects. This, in turn, changes behavioral reactions, anxiety and stress response. In this regard, it seems promising to use regulatory peptide-based neurotropic drugs including Selank to correct stress-induced dysbiosis. Our study was aimed at assessing state of rat colon microbiocenosis in modelled chronic restraint stress and treated with Selankby using 65 Wistar male rats divided into five groups (per 13 rats in each): group 1 — rats injected with saline; group 2 — injected with saline and induced chronic restraint stress; group 3–5 — administered with Selank at dose of 80 μg/kg, 250 μg/kg and 750 μg/kg body weight, respectively, and induced chronic restraint stress. Quantitative and qualitative study of animal colon microbiota was carried out according to the method by L.I. Kafarskaja and V.M. Korshunov. Identification of microorganisms was carried out by using a Maldi Biotyper Microflex mass spectrometer (Bruker, United States). Microbial species-specific composition was presented as lg CFU/g mass of examined sample. For each identified microbial genus, the relative mean and frequency of occurrence were calculated. Statistical significance of differences in mean values was determined by using Student’s t-test. Chronic restraint stress in the experiment did not result in affecting dominant microbiota species in rat colon nor reduce their frequency, however, it significantly influenced examined parameters for commensal microbiota disturbing pattern of pathogenic bacterial strains. Use of Selank led to the reversing changes in composition of colonic microbiocenosis caused by stress model. Moreover, magnitude of parameters examined in experiment after applying Selank at dose of 750 μg/kg reached those in non-stressed animals. Thus, effects related to Selank administration may presumably be mediated due to both central and peripheral effects including immunotropic and anti-inflammatory activities which contributed to restoring colon microbiocenosis composition in stress model.

About the authors

A. Yu. Mukhina

Kursk State Medical University

Author for correspondence.
Email: 111ms@mail.ru

Aleksandra Yu. Mukhina, Assistant Professor, Department of Microbiology, Virology, Immunology

305041, Kursk, K. Marksa str., 3.

Phone: +7 (4712) 58-81-43 (office); +7 919 135-29-08 (mobile).

Russian Federation

O. A. Medvedeva

Kursk State Medical University

Email: fake@neicon.ru

PhD, MD (Biology), Associate Professor, Professor of the Department of Microbiology, Virology, Immunology

Kursk Russian Federation

M. V. Svishcheva

Kursk State Medical University

Email: fake@neicon.ru

PhD Student, Department of Microbiology, Virology, Immunology

Kursk 

Russian Federation

A. V. Shevchenko

Kursk State Medical University

Email: fake@neicon.ru

PhD (Medicine), Assistant Professor, Department of Microbiology, Virology, Immunology

Kursk 

Russian Federation

N. N. Efremova

Kursk State Medical University

Email: fake@neicon.ru

PhD (Biology), Associate Professor, Department of Microbiology, Virology, Immunology

Kursk 

Russian Federation

I. I. Bobyntsev

Kursk State Medical University

Email: fake@neicon.ru

PhD, MD (Medicine), Professor, Head of the Department of Pathophysiology

Kursk 

Russian Federation

P. V. Kalutsky

Kursk State Medical University

Email: fake@neicon.ru

PhD, MD (Medicine), Professor, Head of the Department of Microbiology, Virology, Immunology

Kursk 

Russian Federation

L. A. Andreeva

Institute of Molecular Genetics, Russian Academy of Sciences

Email: fake@neicon.ru

PhD (Chemistry), Head of the Regulatory Peptide Sector, Department of Physiologically Active Substances Chemistry

Moscow

Russian Federation

N. F. Myasoedov

Institute of Molecular Genetics, Russian Academy of Sciences

Email: fake@neicon.ru

RAS Full Member, PhD, MD (Chemistry), Head of the Department of Physiologically Active Substances Chemistry

Moscow

Russian Federation

References

  1. Андреева Л.А., Мезенцева М.В., Наровлянский А.Н., Нагаев И.Ю., Шаповал И.М., Щербенко В.Э., Руссу Л.И., Мясоедов Н.Ф. Перспективы создания новых пептидных лекарственных препаратов, обладающих противоинфекционной и иммуномодулирующей активностью // Инфекция и иммунитет. 2011. Т. 1, № 2. С. 171–176. doi: 10.15789/2220-7619-2011-2-171-176
  2. Богданова Е.А., Несвижский Ю.В., Воробьев А.А. Исследование пристеночной микрофлоры желудочно-кишечного тракта крыс при пероральном введении пробиотических препаратов // Вестник РАМН. 2006. № 2. С. 6–10.
  3. Козловский И.И., Андреева Л.А., Козловская М.M., Надорова А.В., Колик Л.Г. О роли опиоидной системы в формировании особенностей анксиолитического действия пептидного препарата cеланка // Экспериментальная и клиническая фармакология. 2012. Т. 75, № 2. С. 10–13. doi: 10.30906/0869-2092-2012-75-2-10-13
  4. Коломин Т.А., Агапова Т.Ю., Агниуллин Я.В., Шрам С.И., Шадрина М.И., Сломинский П.А., Лимборская С.А., Мясоедов Н.Ф. Изменение транскрипционного профиля гиппокампа в ответ на введение аналога тафтцина селанка // Журнал высшей нервной деятельности им. И.П. Павлова. 2013. Т. 63, № 3. С. 365–373. doi: 10.7868/S0044467713030052
  5. Мэгарран Э. Экологическое разнообразие и его измерение. М.: Мир, 1992. 184 с.
  6. Учакина О.Н., Учакин П.Н., Мясоедов Н.Ф., Андреева Л.А., Щербенко В.Э., Мезенцева М.В., Габаева М.В., Соколов О.Ю., Зозуля А.А., Ершов Ф.И. Иммуномодулирующее действие cеланка у больных с тревожно-астеническими расстройствами // Журнал неврологии и психиатрии им. С.С. Корсакова. 2008. Т. 108, № 5. С. 71–75.
  7. Crumeyrolle-Arias M., Jaglin M., Bruneau A., Vancassel S., Cardona A., Dauge V., Naudon L., Rabot S. Absence of the gut microbiota enhances anxiety-like behavior and neuroendocrine response to acute stress in rats. Psychoneuroendocrinology, 2014, no. 42, pp. 207–217. doi: 10.1016/j.psyneuen.2014.01.014
  8. De Palma G., Blennerhassett P., Lu J., Deng Y., Park A.J., Green W., Denou E., Silva M.A., Santacruz A., Sanz Y., Surette M.G., Verdu E.F., Collins S.M., Bercik P. Microbiota and host determinants of behavioural phenotype in maternally separated mice. Nat. Commun. 2015, no. 6: 7735. doi: 10.1038/ncomms8735
  9. Galley J.D., Nelson M.C., Yu Z., Dowd S.E., Walter J., Kumar P.S., Lyte M., Bailey M.T. Exposure to a social stressor disrupts the community structure of the colonic mucosa-associated microbiota. BMC Microbiol., 2014, no. 14, p. 189. doi: 10.1186/1471-2180-14-189
  10. Kim M.H., Leem Y.H. Chronic exercise improves repeated restraint stress-induced anxiety and depression through 5HT1A receptor and cAMP signaling in hippocampus. J. Exerc. Nutrition Biochem., 2014, vol. 18, no 1, pp. 97–104. doi: 10.5717/jenb.2014.18.1.97
  11. Moussaoui N., Jacobs J.P., Larauche M., Biraud M., Million M., Mayer E. Chronic early-life stress in rat pups alters basal corticosterone, intestinal permeability, and fecal microbiota at weaning: influence of sex. J. Neurogastroenterol. Motil., 2017, vol. 23, pp. 135–143. doi: 10.5056/jnm16105
  12. Sudo N. Effects of gut microbiota on stress response and behavioral phenotype of the host. Brain Nerve, 2016, vol. 68, no. 6, pp. 595–605. doi: 10.11477/mf.1416200447
  13. Taché Y., Larauche M., Yuan P.Q., Million M. Brain and gut CRF signaling: biological actions and role in the gastrointestinal tract. Curr. Mol. Pharmacol., 2018, vol. 11, pp. 51–71. doi: 10.2174/1874467210666170224095741

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Copyright (c) 2020 Mukhina A.Y., Medvedeva O.A., Svishcheva M.V., Shevchenko A.V., Efremova N.N., Bobyntsev I.I., Kalutsky P.V., Andreeva L.A., Myasoedov N.F.

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