BIOFILM FORMATION INDUCED BY CLINICAL ISOLATES OF MYCOBACTERIUM TUBERCULOSIS
- Authors: Ogarkov O.B.1,2, Suzdalnitsky A.E.3, Khromova P.A.1, Tsyrenova T.A.3, Sokolnikova N.A.3, Zhdanova S.N.1, Koshcheev M.E.3
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Affiliations:
- Scientific Center for Family Health and Human Reproduction Problems, Irkutsk
- Irkutsk State Medical Academy of Continuing Education, Irkutsk
- Irkutsk Regional Clinical Tuberculosis Hospital, Irkutsk
- Issue: Vol 8, No 4 (2018)
- Pages: 435-440
- Section: MOLECULAR BASES OF EPIDEMIOLOGY, DIAGNOSTICS, PREVENTION AND TREATMENT OF INFECTIOUS DISEASES
- Submitted: 13.12.2018
- Published: 30.12.2018
- URL: https://iimmun.ru/iimm/article/view/822
- DOI: https://doi.org/10.15789/2220-7619-2018-4-435-440
- ID: 822
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Full Text
Abstract
Abstract. The data proving low probability of observing Biofilm Formation (BF) by contemporary clinical strains of M. tuberculosis growing on liquid medium in vitro are discussed. A hypothesis about the role of MDR/XDR development hindering BF production was proposed. It was found that strains capable of producing BF grow on Lewenstein–Jensen medium generated R-form specific colonies shaped as a disk with a convex center, “UFO-colonies”. Sixty seven “UFO”- strains were investigated to BF production, resistance to antibiotics and their belonging to the main epidemics clusters of the Beijing genotype (CC1 and CC2-W148). It was shown that MDR/XDR strains were also capable of BF production that, however, was remarkably more frequent in strains of CC1 and CC2-W148 genotypes. Thus, it was hypothesized that BF production might potentially influence an outcome of chronic forms of TB-infection.
Keywords
About the authors
O. B. Ogarkov
Scientific Center for Family Health and Human Reproduction Problems, Irkutsk; Irkutsk State Medical Academy of Continuing Education, Irkutsk
Author for correspondence.
Email: obogarkov@yandex.ru
PhD, MD (Medicine), Head of the Department of Epidemiology and Microbiology
664003, Russian Federation, Irkutsk, Timiryaseva str., 16
Phone: +7 (3952) 20-73-67
РоссияA. E. Suzdalnitsky
Irkutsk Regional Clinical Tuberculosis Hospital, Irkutsk
Email: fake@neicon.ru
Head of the Surgical Department Россия
P. A. Khromova
Scientific Center for Family Health and Human Reproduction Problems, Irkutsk
Email: fake@neicon.ru
Junior Researcher, Department of Epidemiology and Microbiology Россия
T. A. Tsyrenova
Irkutsk Regional Clinical Tuberculosis Hospital, Irkutsk
Email: fake@neicon.ru
Head of the Bacteriological Laboratory Россия
N. A. Sokolnikova
Irkutsk Regional Clinical Tuberculosis Hospital, Irkutsk
Email: fake@neicon.ru
Bacteriologist Россия
S. N. Zhdanova
Scientific Center for Family Health and Human Reproduction Problems, Irkutsk
Email: fake@neicon.ru
PhD (Medicine), Junior Researcher, Department of Epidemiology and Microbiology Россия
M. E. Koshcheev
Irkutsk Regional Clinical Tuberculosis Hospital, Irkutsk
Email: fake@neicon.ru
PhD (Medicine), Chief Physician Россия
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