BIOFILM FORMATION INDUCED BY CLINICAL ISOLATES OF MYCOBACTERIUM TUBERCULOSIS

Cover Page


Cite item

Full Text

Abstract

Abstract. The data proving low probability of observing Biofilm Formation (BF) by contemporary clinical strains of M. tuberculosis growing on liquid medium in vitro are discussed. A hypothesis about the role of MDR/XDR development hindering BF production was proposed. It was found that strains capable of producing BF grow on Lewenstein–Jensen medium generated R-form specific colonies shaped as a disk with a convex center, “UFO-colonies”. Sixty seven “UFO”- strains were investigated to BF production, resistance to antibiotics and their belonging to the main epidemics clusters of the Beijing genotype (CC1 and CC2-W148). It was shown that MDR/XDR strains were also capable of BF production that, however, was remarkably more frequent in strains of CC1 and CC2-W148 genotypes. Thus, it was hypothesized that BF production might potentially influence an outcome of chronic forms of TB-infection.

About the authors

O. B. Ogarkov

Scientific Center for Family Health and Human Reproduction Problems, Irkutsk; Irkutsk State Medical Academy of Continuing Education, Irkutsk

Author for correspondence.
Email: obogarkov@yandex.ru

PhD, MD (Medicine), Head of the Department of Epidemiology and Microbiology

664003, Russian Federation, Irkutsk, Timiryaseva str., 16

Phone: +7 (3952) 20-73-67

Russian Federation

A. E. Suzdalnitsky

Irkutsk Regional Clinical Tuberculosis Hospital, Irkutsk

Email: fake@neicon.ru
Head of the Surgical Department Russian Federation

P. A. Khromova

Scientific Center for Family Health and Human Reproduction Problems, Irkutsk

Email: fake@neicon.ru
Junior Researcher, Department of Epidemiology and Microbiology Russian Federation

T. A. Tsyrenova

Irkutsk Regional Clinical Tuberculosis Hospital, Irkutsk

Email: fake@neicon.ru
Head of the Bacteriological Laboratory Russian Federation

N. A. Sokolnikova

Irkutsk Regional Clinical Tuberculosis Hospital, Irkutsk

Email: fake@neicon.ru
Bacteriologist Russian Federation

S. N. Zhdanova

Scientific Center for Family Health and Human Reproduction Problems, Irkutsk

Email: fake@neicon.ru
PhD (Medicine), Junior Researcher, Department of Epidemiology and Microbiology Russian Federation

M. E. Koshcheev

Irkutsk Regional Clinical Tuberculosis Hospital, Irkutsk

Email: fake@neicon.ru
PhD (Medicine), Chief Physician Russian Federation

References

  1. О совершенствовании противотуберкулезных мероприятий в Российской Федерации: приказ Министерство Здравоохранения Российской Федерации № 109 от 21 марта 2003 г. [On the improvement of tuberculosis measures in the Russian Federation: Order of the Russia Ministry of Health, March 21, 2003 No. 109]. URL: http://www.consultant.ru/document/cons_doc_LAW_100829 (10.09.2018)
  2. Об унификации микробиологических методов исследования при туберкулезе: приказ Минздрава СССР № 558 от 8 июня 1978 г. [On the unification of microbiological research methods for tuberculosis: order of the USSR Ministry of Health No. 558, June 8, 1978]
  3. Огарков О.Б., Бадлеева М.В., Белькова Н.Л., Адельшин Р.В., Цыренова Т.А., Хромова П.А., Синьков В.В., Костюнин К.Ю., Дашацыренова С.Б., Кощеев М.Е., Зарбуев А.Н., Жданова С.Н. Феномен образования биопленок штаммами Brevibacillus spp. и Bacillus spp. в присутствии клинических штаммов Mycobacterium tuberculosis // Молекулярная генетика, микробиология и вирусология. 2017. Т. 35, № 3. С. 98–103. [Ogarkov O.B., Badleeva M.V., Belkova N.L., Adelshin R.V., Tsyrenova T.A., Khromova P.A., Sinkov V.V., Kostjunin K.Yu., Dashatsyrenova S.B., Koshcheyev M.E., Zarbuev A.N., Zhdanova S.N. The phenomenon of the formation of biofilms by Brevibacillus spp. and Bacillus spp. in the presence of clinical strains of Mycobacterium tuberculosis. Molekulyarnaya genetika, mikrobiologiya i virusologiya = Molecular Genetics, Microbiology and Virology, 2017, vol. 35, no. 3, pp. 98–103. doi: 10.3103/S0891416817030065 (In Russ.)]
  4. Синьков В.В., Савилов Е.Д., Огарков О.Б. Реконструкция эпидемической истории «пекинского» генотипа Mycobacterium tuberculosis в России и странах бывшего СССР по результатам сполиготипирования // Молекулярная генетика, микробиология и вирусология. 2011. № 3. С. 25–29. [Sinkov V.V., Savilov E.D., Ogarkov O.B. Reconstruction of the epidemic history of the Beijing genotype of Mycobacterium tuberculosis in Russia and former soviet countries using spoligotyping. Molekulyarnaya genetika, mikrobiologiya i virusologiya = Molecular Genetics, Microbiology and Virology, 2011, no. 3, pp. 25–29. doi: 10.3103/S0891416811030050 (In Russ.)]
  5. Хромова П.А., Огарков О.Б., Жданова С.Н., Синьков В.В., Моисеева Е.Я., Цыренова Т.А., Кощеев М.Е., Зоркальцева Е.Ю., Савилов Е.Д. Выявление высокотрансмиссивных генотипов возбудителя в клиническом материале для прогноза неблагоприятного течения туберкулеза // Клиническая лабораторная диагностика. 2017. Т. 62, № 10. С. 622– 627. [Khromova P.A., Ogarkov O.B., Zhdanova S.N., Sinkov V.V., Moiseeva E.Ya., Tzyrenova T.A., Koscheev M.E., Zorkaltseva E. Yu., Savilov E.D. The detection of highly-transmissible genotypes of agent in clinical samples for prognosis of unfavorable course of tuberculosis. Klinicheskaya laboratornaya diagnostika = Clinical Laboratory Diagnostics, 2017, vol. 62, no. 10, pp. 622–627. doi: 10.18821/0869-2084-2017-62-10-622-627 (In Russ.)]
  6. Dalton J.P., Uy B., Phummarin N., Copp B.R., Denny W.A., Swift S., Wiles S. Effect of common and experimental anti-tuberculosis treatments on Mycobacterium tuberculosis growing as biofilms. Peer J., 2016, vol. 22, no. 4: e2717. doi: 10.7717/peerj.2717
  7. Esteban J., García-Coca M. Mycobacterium biofilms. Front Microbiol., 2018, vol. 18, no. 8, p. 2651. doi: 10.3389/fmicb.2017.02651
  8. Hilz H., Wiegers U., Adamietz P. Stimulation of proteinase K action by denaturing agents: application to the isolation of nucleic acids and the degradation of “masked” proteins. Eur. J. Biochem., 1975, vol. 56, no. 1, pp. 103–108. doi: 10.1111/j.1432-1033.1975.tb02211.x
  9. Jindani A., Dore C.J., Mitchison D.A. Bactericidal and sterilizing activities of antituberculosis drugs during the first 14 days. Am. J. Respir. Crit. Care Med., 2003, vol. 167, pp. 1348–1354. doi: 10.1164/rccm.200210-1125OC
  10. Merker M., Blin C., Mona S., Duforet-Frebourg N., Lecher S., Willery E., Blum M.G., Rüsch-Gerdes S., Mokrousov I., Aleksic E., Allix-Béguec C., Antierens A., Augustynowicz-Kopeć E., Ballif M., Barletta F., Beck H.P., Barry C.E. 3rd, Bonnet M., Borroni E., Campos-Herrero I., Cirillo D., Cox H., Crowe S., Crudu V., Diel R., Drobniewski F., Fauville-Dufaux M., Gagneux S., Ghebremichael S., Hanekom M., Hoffner S., Jiao W.W., Kalon S., Kohl T.A., Kontsevaya I., Lillebæk T., Maeda S., Nikolayevskyy V., Rasmussen M., Rastogi N., Samper S., Sanchez-Padilla E., Savic B., Shamputa I.C., Shen A., Sng L.H., Stakenas P., Toit K., Varaine F., Vukovic D., Wahl C., Warren R., Supply P., Niemann S., Wirth T. Evolutionary history and global spread of the Mycobacterium tuberculosis Beijing lineage. Nat. Genet., 2015, vol. 47, no. 3, pp. 242–249. doi: 10.1038/ng.3195
  11. Ojha A.K., Baughn A.D., Sambandan D., Hsu T., Trivelli X., Guerardel Y., Alahari A., Kremer L., Jacobs W.R. Jr, Hatfull G.F. Growth of Mycobacterium tuberculosis biofilms containing free mycolic acids and harbouring drug-tolerant bacteria. Mol. Microbiol., 2008, vol. 69, pp. 164–174. doi: 10.1111/j.1365-2958.2008.06274.x
  12. Orme I.M. A new unifying theory of the pathogenesis of tuberculosis. Tuberculosis (Edinb), 2014, vol. 94, no. 1, pp. 8–14. doi: 10.1016/j.tube.2013.07.004
  13. Shitikov E., Kolchenko S., Mokrousov I., Bespyatykh J., Ischenko D., Ilina E., Govorun V. Evolutionary pathway analysis and unified classification of East Asian lineage of Mycobacterium tuberculosis. Sci. Rep., 2017, vol. 7, pp. 9227. doi: 10.1038/s41598-017-10018-5
  14. Wallis R.S., Patil S., Cheon S.H., Edmonds K., Phillips M., Perkins M.D., Joloba M., Namale A., Johnson J.L., Teixeira L., Dietze R., Siddiqi S., Mugerwa R.D., Eisenach K., Ellner J.J. Drug tolerance in Mycobacterium tuberculosis. Antimicrob. Agents Chemother., 1999, vol. 43, no. 11, pp. 2600–2606.
  15. Wayne L.G., Sohaskey C.D. Nonreplicating persistence of Mycobacterium tuberculosis. Annu. Rev. Microbiol., 2001, vol. 55, pp. 139–163.
  16. Xiang X., Deng W., Liu M., Xie J. Mycobacterium biofilms: factors involved in development, dispersal, and therapeutic strate gies against biofilm-relevant pathogens. Crit. Rev. Eukaryot. Gene Expr., 2014, vol. 24, no. 3, pp. 269–279. doi: 10.1615/CritRevEukaryotGeneExpr.2014010545

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2018 Ogarkov O.B., Suzdalnitsky A.E., Khromova P.A., Tsyrenova T.A., Sokolnikova N.A., Zhdanova S.N., Koshcheev M.E.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 64788 от 02.02.2016.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies