BIOFILM FORMATION INDUCED BY CLINICAL ISOLATES OF MYCOBACTERIUM TUBERCULOSIS

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Abstract

Abstract. The data proving low probability of observing Biofilm Formation (BF) by contemporary clinical strains of M. tuberculosis growing on liquid medium in vitro are discussed. A hypothesis about the role of MDR/XDR development hindering BF production was proposed. It was found that strains capable of producing BF grow on Lewenstein–Jensen medium generated R-form specific colonies shaped as a disk with a convex center, “UFO-colonies”. Sixty seven “UFO”- strains were investigated to BF production, resistance to antibiotics and their belonging to the main epidemics clusters of the Beijing genotype (CC1 and CC2-W148). It was shown that MDR/XDR strains were also capable of BF production that, however, was remarkably more frequent in strains of CC1 and CC2-W148 genotypes. Thus, it was hypothesized that BF production might potentially influence an outcome of chronic forms of TB-infection.

About the authors

O. B. Ogarkov

Scientific Center for Family Health and Human Reproduction Problems, Irkutsk; Irkutsk State Medical Academy of Continuing Education, Irkutsk

Author for correspondence.
Email: obogarkov@yandex.ru

PhD, MD (Medicine), Head of the Department of Epidemiology and Microbiology

664003, Russian Federation, Irkutsk, Timiryaseva str., 16

Phone: +7 (3952) 20-73-67

Russian Federation

A. E. Suzdalnitsky

Irkutsk Regional Clinical Tuberculosis Hospital, Irkutsk

Email: fake@neicon.ru
Head of the Surgical Department Russian Federation

P. A. Khromova

Scientific Center for Family Health and Human Reproduction Problems, Irkutsk

Email: fake@neicon.ru
Junior Researcher, Department of Epidemiology and Microbiology Russian Federation

T. A. Tsyrenova

Irkutsk Regional Clinical Tuberculosis Hospital, Irkutsk

Email: fake@neicon.ru
Head of the Bacteriological Laboratory Russian Federation

N. A. Sokolnikova

Irkutsk Regional Clinical Tuberculosis Hospital, Irkutsk

Email: fake@neicon.ru
Bacteriologist Russian Federation

S. N. Zhdanova

Scientific Center for Family Health and Human Reproduction Problems, Irkutsk

Email: fake@neicon.ru
PhD (Medicine), Junior Researcher, Department of Epidemiology and Microbiology Russian Federation

M. E. Koshcheev

Irkutsk Regional Clinical Tuberculosis Hospital, Irkutsk

Email: fake@neicon.ru
PhD (Medicine), Chief Physician Russian Federation

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Copyright (c) 2018 Ogarkov O.B., Suzdalnitsky A.E., Khromova P.A., Tsyrenova T.A., Sokolnikova N.A., Zhdanova S.N., Koshcheev M.E.

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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