Anesthesia of laboratory animals in manufacturing of diagnostic and preventive biomedicines

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Abstract

Preparations such as XilaVet, Zoletil 100 as well as Aeranne (Isoflurane) are successfully applied for animal anesthesia in veterinary practice. We assessed a possibility of using parenteral narcosis with Zoletil 100 in combination with muscle relaxant Xila for producer-rabbits involved in manufacturing of natural rabbit serum subsequently deployed for production of diagnostic serum and immunoglobulin preparations. Administration of preparations into auricular vein is easy to do, while animals are sedated immediately allowing for safe fixation on restraining table and causing no additional stress for biomodels. This type of narcosis provides for expected depth of anesthesia and its maintenance until the end of blood-letting procedure. Parameters characterizing the state of cardiovascular system due to anesthetic products remained within the permitted limits. These preparations do not reduce heart beat rate allowing for collecting sufficient blood volumes. Application of inhalation anesthesia with Aeranne in laboratory animals provides for the specified depth of anesthesia and its maintenance until the end of the whole procedure. However, it requires specialized equipment and highly trained personnel with appropriate skills. Usage of Xila as a mono narcosis is not recommended as exhibits weak analgesic effects and strong hypotensive activity by decreasing quantity of collected blood volume. It was found that anesthetics such as Xila, Zoletil 100, Aeranne did not affect specific activity of immune sera in case of total dehematizing procedure. Moreover, antibody titers were not declined throughout entire observation (12 months) period and complied with the requirements of regulatory documentation. In addition, a feasibility of replacing old-fashioned anesthesia method with diethyl ether for a combination of safer contemporary preparations of Zoletil 100 and Xila was demonstrated while manufacturing tableted chemical cholera vaccine in experimental series with suckling rabbits used at diverse stages of raw material verification during surgical interventions. Xila, Zoletil 100, and Aeranne examined by us had no impact on the amount of blood obtained from donor-animals, immunological properties of the sera and ready-to-use diagnostic preparations. Such drugs were safe for all-age animals that comply with the requirements to anesthesia of animal biomodels and producer-animals in manufacturing of immunobiological preparations. Thus, our study allowed to conduct experiments with laboratory animals in a more humane manner.

About the authors

A. P. Semakova

Federal Government Health Institution “Russian Research Anti-Plague Institute “Microbe” of the Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare

Author for correspondence.
Email: Semakova.ap@gmail.com

PhD (Biology), Senior Research Officer of the Department of Experimental Animals with Vivarium.

410005, Saratov, Universitetskaya str., 46, Phone: +7 (8452) 26-21-31 (office)

Russian Federation

V. G. Germanchuk

Federal Government Health Institution “Russian Research Anti-Plague Institute “Microbe” of the Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare

Email: rusrapi@microbe.ru

PhD, MD (Medicine), Associate Professor, Head of the Department of Experimental Animals with Vivarium.

Saratov

Russian Federation

V. V. Rogozhin

Federal Government Health Institution “Russian Research Anti-Plague Institute “Microbe” of the Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare

Email: rusrapi@microbe.ru

Junior Researcher, Department of Diagnostic Preparations.

Saratov

Russian Federation

N. Y. Shavina

Federal Government Health Institution “Russian Research Anti-Plague Institute “Microbe” of the Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare

Email: rusrapi@microbe.ru

Researcher, Department of Experimental Animals with Vivarium.

Saratov

Russian Federation

M. V. Ovchinnikova

Federal Government Health Institution “Russian Research Anti-Plague Institute “Microbe” of the Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare

Email: rusrapi@microbe.ru

PhD (Biology), Head of the Department of Diagnostic Preparations.

Saratov

Russian Federation

T. Y. Kirilova

Federal Government Health Institution “Russian Research Anti-Plague Institute “Microbe” of the Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare

Email: rusrapi@microbe.ru

Researcher, Department of Diagnostic Preparations.

Saratov

Russian Federation

L. F. Livanova

Federal Government Health Institution “Russian Research Anti-Plague Institute “Microbe” of the Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare

Email: rusrapi@microbe.ru

PhD (Biology), Senior Researcher, Department of Prophylactic Preparations.

Saratov

Russian Federation

N. I. Belyakova

Federal Government Health Institution “Russian Research Anti-Plague Institute “Microbe” of the Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare

Email: rusrapi@microbe.ru

PhD (Medicine), Senior Researcher, Department of Prophylactic Preparations.

Saratov

Russian Federation

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Copyright (c) 2020 Semakova A.P., Germanchuk V.G., Rogozhin V.V., Shavina N.Y., Ovchinnikova M.V., Kirilova T.Y., Livanova L.F., Belyakova N.I.

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