VNTR loci as indicators of proline-dependent plague microbe strains (Yersinia pestis) in the central caucasian mountain natural plague focus

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Abstract

Y. pestis MLVA typing is used both to seek for similarities and differences between individual isolates upon conducting epidemiological investigations as well as for clonal clustering of intraspecies phylogenetic groups while analyzing microevolution and taxonomy issues. It cannot be ruled out that the most variable loci may be indicators allowing to approximate the unique strain-related properties circulating in certain natural plague foci. The Central Caucasian Highland Natural Plague Focus distinguished by heterogeneity of the circulating strains therein, including proline pro- and auxo-trophy, may represent a convenient model for testing this hypothesis. The purpose of our work was to assess the frequencies of the VNTR alleles associated with proline dependence among the Y. pestis strain VNTR loci, determined during previous MLVA-25 typing in the Central Caucasian Highland Natural Plague Focus. The main task was to identify the most informative sets of VNTR loci suitable for predicting proline pro— and auxotrophy (pro+, pro—). It was found that the loci ms45, ms56, ms46, ms07, ms69, ms62 displayed peak variability by allele frequencies and/or exhibited significant differences of mean allele frequencies in the pro— and pro+ strains. In particular, it was showed that the alleles of the ms45 locus contained 6 tandem repeats suggesting probability for pro+ reaching 0.944, whereas the alleles of the ms45 locus contained 7 tandem repeats with expected probability for pro— reaching 0.783. Moreover, the ms56 and ms46 contained 9 and more than 18 tandem repeats, respectively, thereby pointing at probability for pro+ equal to 0.933 and 0.818, respectively. Diagnostics for pro+/pro— phenotype by using specific statistical methods demonstrated statistical error 13.33% and 26.67% for the pro— and pro+ strains, respectively. All pro+ strains bearing a 6 tandem repeat complex from the ms45 locus, 9 tandem repeats derived from the ms56 locus and ms46 locus-derived 29—30 tandem repeats were accurately diagnosed solely based on these 3 loci. Thus, it is possible to predict some properties of Y. pestis strains based on determining the allele frequencies. While the number of MLVA typed plaque strains isolated in such natural focus has been progressively increased, it may be expected that opportunities for prognosing their properties based on determining locus tandem repeat composition having diagnostic value would be elevated.

About the authors

V. M. Dubyanskiy

Stavropol Research Anti-Plague Institute

Author for correspondence.
Email: dvmplague@gmail.com
ORCID iD: 0000-0003-3817-2513

Vladimir M. Dubyanskiy - PhD, MD (Biology), Head of Department of Epizootic Monitoring and Forecasting.

355035, Stavropol, Sovetskaya, 13-15, Phone: +7 (8652) 26-03-12

Russian Federation

A. S. Volynkina

Stavropol Research Anti-Plague Institute

Email: volyn444@mail.ru
ORCID iD: 0000-0001-5554-5882

Researcher of Laboratory of Natural Focal Infections.

Stavropol

Russian Federation

A. P. Anisimov

Federal Budget Institution of Science «State Research Center for Applied Microbiology & Biotechnology»

Email: anisimov@obolensk.org
ORCID iD: 0000-0002-5499-7999

PhD, MD (Medicine), Professor, Deputy Director for Science.

Obolensk

Russian Federation

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Copyright (c) 2020 Dubyanskiy V.M., Volynkina A.S., Anisimov A.P.

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