Cover Page

Cite item


The article presents the results of a study related to increasing the efficiency of phage isolation of bacteria of the species K. oxytoca, by developing the optimal composition of the medium used in the work. In scientific research, in almost all methods associated with the isolation of bacteriophages, meat-peptone broth and meat-peptone agar are used as the nutrient basis. The peculiarities of growth and cultivation of microorganisms create certain difficulties for the isolation of phages active against bacteria of the species K. oxytoca. The selection of components and the creation of an environment that would ensure the optimal growth of both the bacterial culture and the reproduction of the virus makes it possible to facilitate the isolation of bacteriophages. The number of bacterial strains used in the work was 7. All strains of cultures were obtained from the Museum of the Department of Microbiology, Virology, Epizootology and Veterinary and Sanitary Expertise of the Federal State Budget Educational Institution of Higher Education “Ulyanovsk State Agrarian University named after P.A. Stolypin”. The studies included 2 main stages. The first stage consisted in isolation of bacteriophages by the method of isolation from the external environment by the method of Adelson L.I., Lyashenko E.A. The material for the studies were samples: soil, sewage sample, fecal samples (2). Only 4 samples. According to the chosen method, the sowing of the putative phagolysate was carried out on meat-peptone agar (1.5%) and the agar for isolating bacteriophages (Aph) (1.5%). A positive result was the presence on the environment of negative colonies, clearly visible on the matt background of deep growth of bacteria. A negative result is a continuous growth (“lawn”) of bacterial culture. As a control, the culture of the microorganism studied was used for the media. In the course of the conducted studies for the first stage, 2 bacteriophages were isolated, active against the desired species of microorganism. The second stage consisted in a comparative analysis of the indices of cultivation of the isolated phages on standard meat-peptone agar and the proposed medium for isolation of bacteriophages. The main biological properties of the phages studied were also determined: morphology of negative colonies, lytic activity, specificity. The negative colonies formed on the compared media were classified as one type. The lytic activity of the studied phages on meat-peptone agar and Aph medium was the same. According to Grazia 2 × 107 BOE/ml for bacteriophage KO-3 UGSHA, (3–4) × 108BOE/ml for bacteriophage KO-8724 UGSHA. The isolated bacteriophages showed no lytic activity against strains of heterogeneous crops (Escherichia spp., Citrobacter spp., Serratia spp., Hafnia spp., Yersinia spp.).


About the authors

G. R. Sadrtdinova

Ulyanovsk State Agrarian University named after P.A. Stolypin, Ulyanovsk region

Author for correspondence.
Email: sadrtdinova-guzlik@yandex.ru
Assistant Professor, Department of Microbiology, Virology, Epizootology and Veterinary Sanitation Inspection Russian Federation


  1. Бульканова Е.А., Золотухин С.Н. Выделение бактериофагов рода Klebsiella из сточных вод // Вестник Ульяновской государственной сельскохозяйственной академии. 2004. № 12. С. 40–42. [Bulkanova E.A., Zolotukhin S.N. Isolation of bacteriophages of the genus Klebsiella from wastewater. Vestnik Ul’yanovskoi gosudarstvennoi sel’skokhozyaistvennoi akademii = Vestnik of Ulyanovsk State Agricultural Academy, 2004, no. 12, pp. 40–42. (In Russ.)]
  2. Васильев Д.А., Золотухин С.Н., Садртдинова Г.Р. Сравнительная эффективность методов выделения бактериофагов Klebsiella oxytoca // Вестник Ульяновской государственной сельскохозяйственной академии. 2015. № 4 (32). С. 68–72. [Vasilyev D.A., Zolotukhin S.N., Sadrtdinova G.R. Comparative effectiveness of methods of isolation of bacteriophages Klebsiella oxytoca. Vestnik Ul’yanovskoi gosudarstvennoi sel’skokhozyaistvennoi akademii = Vestnik of Ulyanovsk State Agricultural Academy, 2015, no. 4 (32), pp. 68–72. (In Russ.)]
  3. Захаренко С.М. Бактериофаги: современные аспекты применения, перспективы на будущее // Медицинский совет. 2013. № 10. С. 72–75. [Zakharenko S.M. Bacteriophages: present and future aspects of use. Meditsinskii sovet = Medical Advice, 2013, no. 10, pp. 72–75. (In Russ.)]
  4. Золотухин Д.С., Васильев Д.А., Золотухин С.Н., Семенов А.М., Романова Е.М. Выделение, селекция и изучение биологических свойств бактериофагов Hafnia alvei // Вестник ветеринарии. 2013. № 1 (64). С. 68–70. [Zolotukhin D.S., Vasilyev D.A., Zolotukhin S.N., Semenov A.M., Romanova E.M. The isolation, selection and observation of biological characteristics of Hafnia alvei bacteriophages. Vestnik veterinarii = Herald of Veterinary Medicine, 2013, no. 1 (64), pp. 68–70. (In Russ.)]
  5. Катмакова Н.П., Золотухин С.Н., Васильев Д.А. Поиск и селекция псевдотуберкулезных бактериофагов // Ветери-нарная медицина. 2009. № 4. С. 19–20. [Katmakova N.P., Zolotukhin S.N., Vasilyev D.A. The search and selection of bacteriophages Yersinia pseudotuberculosis strains. Veterinarnaya meditsina = Veterinary Medicine, 2009, no. 4, pp. 19–20. (In Russ.)]
  6. Ляшенко Е.А., Золотухин С.Н., Васильев Д.А. Разработка и применение фагового биопрепарата для диагностики клебсиеллезной инфекции // Вестник ветеринарии. 2011. № 4 (59). С. 90–92. [Lyashenko E.A., Zolotukhin S.N., Vasilyev D.A. Development and application of the bacteriophage for klebsiellos diagnosis. Vestnik veterinarii = Herald of Veterinary Medicine, 2011, no. 4 (59), pp. 90–92. (In Russ.)]

Copyright (c) 2018 Sadrtdinova G.R.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 64788 от 02.02.2016.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies