COMPARATIVE ANALYSIS OF METHODS FOR IDENTIFICATION OF NONTUBERCULOUS MYCOBACTERIA ISOLATED FROM CLINICAL MATERIAL

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Abstract

Recently there has been a significant increase in the incidence of mycobacteriosis, which is due to an increase in the proportion of immunosuppressed patients, the presence of these various comorbid conditions, as well as the improvement of diagnostic methods. Selecting the most accurate method of identification is extremely important in determining treatment strategy of patients. The aim of the study was to conduct a comparative analysis of modern methods of identification NTMB isolated from clinical specimens in 2015 in the Samara region. The work was carried out identification of 78 strains of microorganisms. Laboratory diagnosis was carried out using the DNA hybridization method and MALDI-ToF mass spectrometry. When microbial identification using MALDI-ToF mass spectrometry was isolated 16 strains (20.5%) M. kansasii; 11 strains (14.1%) M. avium and M. fortuitum; 9 strains (11.5%) M. gordonae; strain 3 (3.8%) M. peregrinum, M. szulgai, M. chimera intracellulare group, strain 2 (2.6%) M. abscessus, M. septicum, M. paragordonae, M. senegalence, 1 strain (1.3%) M. chelonae, M. frederiksbergense, M. monacense, M. lentiflavum. By using mass spectrometry, it was identified 15 types NTMB compared with 9 types — by DNA hybridization. Full match identification results was observed only in 45 (57.7%) strains of divergent strains were found in 16 (20.5%). Most often when using the DNA hybridization method, discrepancy was detected in slow-growing cultures (9 strains) with a predominance of microorganisms identified as M. gordonae. Among the representatives of fast-growing NTMB, seven investigations were identified in the identification, more often among representatives of the M. fortuitum and M. peregrinum groups. Particular attention should be paid to the identification of the M. kansasii strain by a molecular genetic method, which mass spectrometry was defined as M. bovis. Both cultures of M. tuberculosis complex, which were identified by MALDI-ToF spectrometry, DNA hybridization were not determined to species. 17 (21.8%) of microbial strains which have been identified using the method of DNA hybridization, identified by spectrometry, including slow-growing microorganisms, non-mycobacteria strains seven (9.0%): Gordonia rubriperticta, Nocardia forcinica, Tsukumurella spp., Rhodotorula mucilaginosa. Accurate species identification NTMB is fundamental to determine the tactics of treatment of patients with mycobacteriosis. Due to this rather limited possibility of identification of non-tuberculous mycobacteria, using a DNA-hybridization method is inadequate to date. The introduction of new techniques, such as MALDI-ToF spectrometry, can identify a greater number of species of nontuberculous mycobacteria, as well as other types of slow-growing microorganisms having similarities with mycobacteria on cultural and morphological properties, which significantly increases the diagnostic capabilities of laboratories.

About the authors

A. V. Lyamin

Samara State Medical University

Author for correspondence.
Email: avlyamin@rambler.ru

Artem V. Lyamin - PhD (Medicine), Associate Professor, Department of General and Clinical Microbiology, Immunology and Allergology.

443079, Самара, ул. Гагарина, 18. Тел.: 8 (846) 260-33-61

Russian Federation

D. D. Ismatullin

Samara State Medical University

Email: fake@neicon.ru
6th year student of the Faculty of Medicine and Prevention

A. V. Zhestkov

Samara State Medical University

Email: fake@neicon.ru

PhD, MD (Medicine), Professor, Head of the Department of General and Clinical Microbiology, Immunology and Allergology

A. M. Kovalyov

Samara Regional Clinical Tuberculosis Dispensary named N.V. Postnikov

Email: fake@neicon.ru

PhD (Biology), Biologist, Bacteriological Laboratory, N.V. Postnikov

L. A. Baryshnikova

Samara Regional Clinical Tuberculosis Dispensary named N.V. Postnikov

Email: fake@neicon.ru
PhD, MD (Medicine), Deputy Chief Physician

S. S. Nenjajkin

Samara State Medical University

Email: fake@neicon.ru

Head of the Department of Infectious Safety and Hygiene

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Copyright (c) 2017 Lyamin A.V., Ismatullin D.D., Zhestkov A.V., Kovalyov A.M., Baryshnikova L.A., Nenjajkin S.S.

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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