DYNAMICS OF TICKS’ INFESTATION WITH BORRELIA GENUS BACTERIA AND TICK-BORNE ENCEPHALITIS VIRUS IN KIROV REGION

Cover Page


Cite item

Full Text

Abstract

Tick-borne encephalitis and tick-borne borreliosis (or Lyme disease) are one of the most known and the most spread transmissible diseases of human. Nowadays it was proved that these diseases are diagnosed among domestic animals as well. Ixodida ticks are often serve as vectors for these diseases. It is common that only ticks gathered from human after bite are studied for presence of infection. But such approach does not give a full distribution picture of infection among the ticks. Thus, it is necessary to study ticks gathered not only from human but from other sources as well. The aim of this study is to determine the presence of TBEV and Borrelia burgdoferi sensu lato genetic material in ticks depending on its species, sexual identity and object it was gathered from in 2007–2016. The main tick vectors were determined. It was shown that percentage of infected with different diseases ticks fluctuates separately during the period of the study. It was also shown that percentage of infected ticks tends to increase. It is often that only tick female are studied as it stay for a long time after the bite. But it was proved that male ticks can also be a vector for diseases. It was proved that percentage of infected male ticks is increasing. The percentage of infected female ticks is also increasing along with general percentage of infected ticks. Fluctuations of percentage of infected ticks among different species was shown. Such fluctuation can be a sign tick’s areal spreading at unusual territories. It can be related to climate change. The difference of percentage of infected ticks of different species can be related to the study of ticks usual for the area and ticks which migrate to the Kirov region. The percentage of infected ticks was also studied. It was also proved that the maximum percentage of infected ticks had the ones gathered from human and minimal percentage had the ones gathered from plants.

About the authors

E. A. Bessolitsyna

Vyatka State University

Author for correspondence.
Email: fake@neicon.ru

PhD (Biology), Associate Professor, Department of Microbiology, Biological Faculty,

Kirov

Russian Federation

S. A. Volkov

Vyatka State University

Email: volkov210691@mail.ru

PhD Student, Department of Microbiology, Biological Faculty,

610000, Kirov, Moskovskaya str., 36

Russian Federation

F. S. Stolbova

Vyatka State Agricultural Academy

Email: fake@neicon.ru

PhD (Biology), Associate Professor, Department of Ecology and Beekeeping, Biological Faculty,

Kirov

Russian Federation

References

  1. Бессолицына Е.А., Бердинских И.С., Столбова Ф.С., Дармов И.В. Анализ зараженности бактериями рода Borrelia клещей на территории Кировской области // Российский паразитологический журнал. 2012. № 4. C. 41–46. [Bessolitsyna E.A., Berdinskikh I.S., Stolbova F.S., Darmov I.A. The analysis of contamination by bacteria of genus Borrelia ticks in Kirov area. Rossiiskii parazitologicheskii zhurnal = Russian Journal of Parasitology, 2012, no. 4, pp. 41–46. (In Russ.)]
  2. Волков С.А., Бессолицына Е.А., Столбова Ф.С., Дармов И.В. Анализ инфицированности клещей видов Ixodes persulcatus и Dermacentor reticulatus трансмиссивными заболеваниями на территории Кировской области // Инфекция и иммунитет. 2016, Т. 6, № 2. С. 173–178. [Volkov S.A., Bessolytsina E.A., Stolbova F.S., Darmov I.V. Analysis of ticks of Ixodes persulcatus and Dermacentor reticulatus species with transmissible diseases in Kirov region. Infektsiya i immunitet = Russian Journal of Infection and Immunity, 2016, vol. 6, no. 2, pp. 173–178. doi: 10.15789/2220-7619-2016-2-173-178 (In Russ.)]
  3. Манзенюк И.Н., Манзенюк О.Ю. Клещевые боррелиозы (болезнь Лайма): пособие для врачей. Кольцово, 2005. 85 с. [Manzenyuk I.N., Manzenyuk O.Yu. Kleshchevye borreliozy (bolezn’ Laima): posobie dlya vrachei [Lyme borreliosis (Lyme disease): manual for physicians]. Koltsovo, 2005. 85 p.]
  4. Филиппова Н.А. Таежный клещ Ixodes persulcatus Schulze (Acrana, Ixodidae): морфология, систематика, экология, медицинское значение. Л.: Наука, 1985. 416 с. [Filippova N.A. Taiga tick Ixodes persulcatus Schulze (Acrana, Ixodidae): morphology, systematics, ecology, medical importance [Taezhnyi kleshch Ixodes persulcatus Schulze (Acrana, Ixodidae): morfologiya, sistematika, ekologiya, meditsinskoe znachenie]. Leningrad: Nauka, 1985. 416 p.]
  5. Gunther G., Haglund M. Tick-borne encephalopathies: epidemiology, diagnosis, treatment and prevention. CNS Drugs, 2005, vol. 19, no. 12, pp. 1009–1032. doi: 10.2165/00023210-200519120-00004
  6. Lindquist L., Vapalahti O. Tick-borne encephalitis. Lancet, 2008, vol. 371, no. 9627, pp. 1861–1871. doi: 10.1016/S0140-6736(08)60800-4
  7. Margos G., Vollmer S.A., Cornet M., Garnier M., Fingerle V., Wilske B., Bormane A., Vitorino L., Collares-Pereira M., Drancourt M., Kurtenbach K. A new Borrelia species defined by multilocus sequence analysis of housekeeping genes. Appl. Environ. Microbiol., 2009, vol. 75, no. 16, pp. 5410–5416. doi: 10.1128/AEM.00116-09
  8. Pfeffer M., Dobler G. Tick-borne encephalitis virus in dogs — is this an issue? Parasites & Vectors, 2011, vol. 4, no. 1: 59. doi: 10.1186/1756-3305-4-59
  9. Randolph S.E. Tick ecology: processes and patterns behind the epidemiological risk posed by ixodid ticks as vectors. Parasitology, 2004, vol. 129, iss. S1, pp. S37–S65. doi: 10.1017/S0031182004004925
  10. Ruzek D., Stastna Н., Kopecky J., Golovljova I., Grubhoffer L. Rapid subtyping of tick-borne encephalitis virus isolates using multiplex RT-PCR. J. Virol. Meth., 2007, vol. 144, pp. 133–137.
  11. Sahai Н., Anwer Khurshid Н. On analysis of epidemiological data involving A 2X2 contingency table: an overview of fisher’s exact test and yates’ correction for continuity. J. Biopharm. Stat., 1995, vol. 1, no. 1, pp. 43–70. doi: 10.1080/10543409508835098
  12. Sambrook J., Fritch T., Maniatis T. Molecular cloning: a laboratory manual. NY: Cold Spring Harbor Laboratory Press, 1989, 1626 p.
  13. Schouls L.M., Van De Pol I., Rijpkema S.G., Schot C.S. Detection and identification of Ehrlichia, Borrelia burgdorferi sensu lato, and Bartonella species in Dutch Ixodes ricinus ticks. J. Clin. Microbiol., 1999, vol. 37, no. 7, pp. 2215–2222.
  14. Stanek G., Reiter M. The expanding Lyme Borrelia complex — clinical significance of genomic species? Clin. Microbiol. Infect., 2011, vol. 17, no. 4, pp. 487–493. doi: 10.1111/j.1469-0691.2011.03492.x

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2017 Bessolitsyna E.A., Volkov S.A., Stolbova F.S.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 64788 от 02.02.2016.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies