MICROBIAL LANDSCAPE OF MICROFLORA OF A PHARYNX AT PATIENTS WITH TONSILLIT’S PATHOLOGY

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Abstract

The microbial landscape of microflora of a pharynx at patients with tonsillit’s pathology were studied. Materials and methods. 79 patients from GBUZ “Research Institute of Clinical Otorhinolaryngology” (78.5% patients with various forms of chronic tonsillitis and 21.5% of patients without chronic tonsillitis (control group). Among patients of the main group with chronic tonsillitis, at 60 (96.8%) patients there was a diagnosis chronic tonsillitis a toxical-allergic form of 1 degree (TAF I), at 1 (1.6%) the patient — chronic tonsillitis a toxical-allergic form II of degree (TAF II) and at 1 (1.6%) the patient — chronic tonsillitis. Identification of microorganisms carried out on cultural-morphological and biochemical properties. Specific identification of the hardly cultivated microorganisms and Corynebacterium was carried out by MALDI-TOFF MS of BioMerieux VITEK MS MALDI-TOF (“bioMerieux”, France). Identification of the allocated Corynebacterium was carried out by amplification of a gene rpoB and the subsequent direct sequencing. Results. The majority (98.7%) of the allocated microorganisms, treated 27 types and were Gram-positive. It is revealed 159 strains of 29 species of microorganisms, from them 41.4% of strains belonged to the Streptococcus, 19.7% — Staphylococcus, 36.9% — Corynebacterium. Among Streptococcus — 55.4% of S. viridans, 38.4% — S. pyogenes and 3.1% — of S. pneumoniae и S. oralis; Staphylococcus — 64.5% of S. aureus, 32.2% of S. epidermidis and 3.3% of S. hominis. 18 types of Corynebacterium С. tuberculostearicum (17.2% strains), C. рseudodiphtheriticum (15.5% strains) and C. aurimucosum (18.9% strains) are revealed. At 44.3% of the surveyed patients the microflora is presented by a monoculture and at 55.7% associations are revealed. Conclusion. The microflora at patients with tonsillit’s pathology is characterized. At the expressed pathological process in a microbiota of a pharynx the monoculture while existence of associations testifies to less expressed pathological process prevails. 

About the authors

O. Yu. Borisova

G.N. Gabrichevsky Institute of Epidemiology and Microbilogy, Moscow, Russian Federation
Russian National Research Medical University named after N.I. Pirogov, Moscow, Russian Federation

Author for correspondence.
Email: olgborisova@mail.ru

PhD, MD (Medicine), Head of the Laboratory of Diagnostics of Diphtheria and Pertussis Infections of G.N. Gabrichevsky Institute of Epidemiology and Microbiology, Professor of Department of Microbiology and Virology of Russian National Research Medical University named after N.I. Pirogov, Moscow, Russian Federation;

Russian Federation

V. A. Aleshkin

G.N. Gabrichevsky Institute of Epidemiology and Microbilogy, Moscow, Russian Federation

Email: fake@neicon.ru

PhD, MD (Medicine), Professor, Director of G.N. Gabrichevsky Institute of Epidemiology and Microbiology, Moscow, Russian Federation;

Russian Federation

A. S. Pimenova

G.N. Gabrichevsky Institute of Epidemiology and Microbilogy, Moscow, Russian Federation

Email: fake@neicon.ru

Junior Researcher, Laboratory of Diagnostic of Diphtheria and Pertussis Infections of G.N. Gabrichevsky Institute of Epidemiology and Microbiology, Moscow, Russian Federation; 

Russian Federation

A. I. Krukov

Research Institute of Clinical Otorhinolaryngology, Moscow, Russian Federation

Email: fake@neicon.ru

PhD, MD (Medicine), Professor, Director of Research Institute of Clinical Otorhinolaryngology, Moscow, Russian Federation;

Russian Federation

N. L. Kunelskaya

Research Institute of Clinical Otorhinolaryngology, Moscow, Russian Federation

Email: fake@neicon.ru

PhD, MD (Medicine), Professor, Deputy Director for Scientific Work of Research Institute of Clinical Otorhinolaryngology, Moscow, Russian Federation; 

Russian Federation

A. V. Gurov

Research Institute of Clinical Otorhinolaryngology, Moscow, Russian Federation
Russian National Research Medical University named after N.I. Pirogov, Moscow, Russian Federation

Email: fake@neicon.ru

PhD, MD (Medicine), Professor of Department of Otorhinolaryngology, Professor of Department of Microbiology and Virology of Russian National Research Medical University named after N.I. Pirogov, Moscow, Russian Federation;

Russian Federation

G. B. Shadrin

Research Institute of Clinical Otorhinolaryngology, Moscow, Russian Federation

Email: fake@neicon.ru

PhD (Medicine), Head of Department of Research Institute of Clinical Otorhinolaryngology, Moscow, Russian Federation;

Russian Federation

A. S. Tovmasyan

Research Institute of Clinical Otorhinolaryngology, Moscow, Russian Federation

Email: fake@neicon.ru

PhD (Medicine), Leader Scientist of Research Institute of Clinical Otorhinolaryngology, Moscow, Russian Federation;

Russian Federation

B. A. Efimov

Russian National Research Medical University named after N.I. Pirogov, Moscow, Russian Federation

Email: fake@neicon.ru

PhD, MD (Medicine), Professor, Professor of Department of Microbiology and Virology of Russian National Research Medical University named after N.I. Pirogov, Moscow, Russian Federation;

Russian Federation

L. I. Kafarskaya

Russian National Research Medical University named after N.I. Pirogov, Moscow, Russian Federation

Email: fake@neicon.ru

PhD, MD (Medicine), Professor, Head of Department of Microbiology and Virology of Russian National Research Medical University named after N.I. Pirogov, Moscow, Russian Federation

Russian Federation

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Copyright (c) 2015 Borisova O.Y., Aleshkin V.A., Pimenova A.S., Krukov A.I., Kunelskaya N.L., Gurov A.V., Shadrin G.B., Tovmasyan A.S., Efimov B.A., Kafarskaya L.I.

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