Prevalence of hepatitis B and C viral markers among apparently healthy residents of the Socialist Republic of Vietnam (Southern Vietnam)

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Abstract

The aim of this study was to assess the prevalence of serological and molecular biological markers of viral hepatitis B and C among apparently healthy residents of the Southern Vietnam. The study material was represented by 397 blood serum samples collected from apparently healthy residents of the Southern Vietnam. The ELISA examination for presence of HBV and HCV markers involved HBsAg, anti-HBs IgG, anti-HBcore IgG, and anti-HCV qualitative determination. For HBV DNA and HCV RNA detection, nucleic acids were extracted from serum blood, and a test for virus detection was carried out by real-time PCR with hybridization fluorescence detection. Amplification and subsequent sequencing of HBV and HCV were performed using nested PCR with paired overlapping primers jointly flanking the target regions. Analysis of the overall prevalence of serological markers showed that among the apparently healthy individuals anti-HBsAg and anti-HCV antibodies were detected in 12.3% (95% CI: 9.27–15.99%) and 3.27% (95% CI: 1.76–5.53%) of individuals, respectively. The prevalence of HBsAg in men (19.1%) significantly exceeded that of found in women (5.9%), χ2 = 14.688 with p = 0.0001, df = 1, calculated odds ratio OR = 3.751 (95% CI: 1.892–7.439). Among apparently healthy patients, taking into account HBsAg-positive and negative samples, HBV DNA was detected in 26.95% (95% CI: 22.65–31.6%). HBV phylogenetic analysis showed that subtype B4 prevalence comprised 64.49%. Subtypes C1 (14.95%), B2 (9.35%), C2 (6.54%), C3 (0.93%), and C5 (3.74%) were also identified. HCV RNA was detected in 7 samples, which accounted for 1.76% (95% CI: 0.71–3.6%). Phylogenetic analysis showed that all HCV isolates belong to genotype 6, subtype 6a (100%).

About the authors

Yu. V. Ostankova

St. Petersburg Pasteur Institute

Author for correspondence.
Email: shenna1@yandex.ru
ORCID iD: 0000-0003-2270-8897

Yuliia V. Ostankova - PhD (Medicine), Senior Researcher, Laboratory of Molecular Immunology, St. Petersburg Pasteur Institute.

197101, St. Petersburg, Mira str., 14.

Phone: +7 (812) 233-20-92

Russian Federation

A. V. Semenov

Ekaterinburg Research Institute of Viral Infections of SRC VB Vector of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing

Email: alexvsemenov@yahoo.com
ORCID iD: 0000-0003-3223-8219

PhD, MD (Biology), Director of Ekaterinburg Research Institute of Viral Infections of SRC VB Vector of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing.

Ekaterinburg.

Russian Federation

E. B. Zueva

St. Petersburg Pasteur Institute

Email: ezueva75@mail.ru
ORCID iD: 0000-0002-0579-110X

PhD (Biology), Biologist, Department of Diagnostics of HIV Infection and AIDS-associated Diseases, St. Petersburg Pasteur Institute.

St. Petersburg.

Russian Federation

E. N. Serikova

St. Petersburg Pasteur Institute

Email: elena.donetsk.serikova@mail.ru
ORCID iD: 0000-0002-0547-3945

Researcher, Laboratory of Virology and Immunology of HIV Infection, St. Petersburg Pasteur Institute.

St. Petersburg.

Russian Federation

A. N. Schemelev

St. Petersburg Pasteur Institute

Email: tvildorm@gmail.com
ORCID iD: 0000-0002-3139-3674

Junior Researcher, Laboratory of Virology and Immunology of HIV Infection, Postgraduate Student, St. Petersburg Pasteur Institute.

St. Petersburg.

Russian Federation

D. E. Valutite

St. Petersburg Pasteur Institute

Email: dianavalutite008@gmail.com
ORCID iD: 0000-0002-0931-102X

Clinical Laboratory Diagnostics Doctor, Department of Diagnostics of HIV Infection and AIDS-associated Diseases, St. Petersburg Pasteur Institute.

St. Petersburg.

Russian Federation

H. K.T. Huinh

Ho Chi Minh Pasteur Institute

Email: hhkthupasteur@gmail.com
ORCID iD: 0000-0001-9199-6103

Hoang K.T. Huynh - Researcher, Medical Analysis Laboratory Department, Ho Chi Minh Pasteur Institute.

Ho Chi Minh City.

Viet Nam

S. A. Egorova

St. Petersburg Pasteur Institute

Email: fake@neicon.ru

PhD, MD (Medicine), Deputy Director for Innovation, St. Petersburg Pasteur Institute.

St. Petersburg.

Russian Federation

A. A. Totolian

St. Petersburg Pasteur Institute

Email: totolian@pasteurorg.ru
ORCID iD: 0000-0003-4571-8799

Areg A. Totolian - RAS Full Member, PhD, MD (Medicine), Professor, Director of St. Petersburg Pasteur Institute.

St. Petersburg.

Russian Federation

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Copyright (c) 2021 Ostankova Y.V., Semenov A.V., Zueva E.B., Serikova E.N., Schemelev A.N., Valutite D.E., Huinh H.K., Egorova S.A., Totolian A.A.

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