CONTRIBUTION OF LACTOFERRIN, SERUM ALBUMIN, AND SECRETORY IMMUNOGLOBULIN A IN ANTIMICROBIAL ACTIVITY OF BREAST MILK SERUM


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Abstract

The contribution of the antimicrobial activity of sIgA, lactoferrin, α-lactalbumin, serum albumin, and lysozyme to the total antimicrobial activity of serum was evaluated. 66 breast milk samples from healthy women aged from 23 to 45 years collected at different periods of lactation were studied. It was found that as the lactation period increased, not only the total antimicrobial activity (r = - 0.944) decreased, but also the activity of the low molecular weight fraction containing the sum of antimicrobial peptides (r = - 0.950). The median lactoferrin level varied from 3.46 mg/ml in colostrum to 0.94 mg/ml one year after the onset of lactation and correlated with serum activity (r = 0.616). The concentration of sIgA was maximal in the serum of colostrum (5.01 mg/ml) and significantly reduced in mature milk, remaining approximately at the same level in subsequent periods of lactation (1.0 mg/ml). Serum albumin concentration significantly decreased with increase of lactation period (from 5.52 to 4.68 mg/ml) and correlated with serum activity (r = 0.589). Evaluation of the effect of the purified α-lactalbumin on C. albicans cells in the range of 5-20 mg/ ml by spectrophotometry and microscopy showed the absence of fungicidal activity. At the same time, purified lactoferrin, IgA, lysozyme, and serum albumin demonstrated a direct fungicidal effect. The activity of the IgA preparation at a concentration relevant to the sIgA content in the serum of first day colostrum was 50.0%, and in the serum after 12 months - 31.0%; lactoferrin - 26.7% and 3.4%; serum albumin - 15.0% and 17.7%; lysozyme - 0.1% and 1.8%. Thus, the sum of the activities of these polypeptides at concentrations typical for the first-day colostrum was 91.7%, which is comparable to the total activity of this serum - 82.4%. The sum of the activities of these polypeptides, typical for 12 months of lactation, was 53.9%, and the total serum activity relevant to this period was 64.5%. Therefore, IgA and lactoferrin contribute the most significant antimicrobial activity in the serum of colostrum, whereas after 12 months of breastfeeding IgA and serum albumin become most significant.

About the authors

Vera Arzumanian

Federal State Budgetary Scientific Institution «I.Mechnikov Research Institute of Vaccines and Sera», Moscow

Email: veraar@mail.ru
ORCID iD: 0000-0001-9769-1634

Dr. of Biological Sciences, Professor; Head of Laboratory Physiology of Fungi and Bacteria at Federal State Budgetary Scientific Institution «I.Mechnikov Research Institute of Vaccines and Sera», Moscow

Russian Federation

Tatiana Kolyganova

I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow;
Federal State Budgetary Scientific Institution «I.Mechnikov Research Institute of Vaccines and Sera», Moscow;

Author for correspondence.
Email: kolyganova_t_i@staff.sechenov.ru
ORCID iD: 0000-0002-9065-9786

Assistant at the Department of Microbiology, Virology, and Immunology at  I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow; Research assistant at Laboratory Physiology of Fungi and Bacteria at Federal State Budgetary Scientific Institution «I.Mechnikov Research Institute of Vaccines and Sera», Moscow

Russian Federation

Oxana Svitich

Federal State Budgetary Scientific Institution «I.Mechnikov Research Institute of Vaccines and Sera», Moscow; I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow

Email: svitichoa@yandex.ru
ORCID iD: 0000-0003-1757-8389

Dr. of Medical Sciences, Professor, Corresponding Member Russian Academy of Sciences; Director of Federal State Budgetary Scientific Institution «I.Mechnikov Research Institute of Vaccines and Sera», Moscow; Professor at the Department of Microbiology, Virology, and Immunology at  I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow;

Russian Federation

Pavel Samoylikov

Federal State Budgetary Scientific Institution «I.Mechnikov Research Institute of Vaccines and Sera», Moscow;

Email: samoilikov@mail.ru
ORCID iD: 0000-0003-3580-3199

Ph.D.  of Medical Sciences; Senior Researcher at Allergy diagnostics laboratory at Federal State Budgetary Scientific Institution «I.Mechnikov Research Institute of Vaccines and Sera», Moscow

Russian Federation

Svetlana Konanykhin

Federal State Budgetary Scientific Institution «I.Mechnikov Research Institute of Vaccines and Sera», Moscow

Email: sdieta@yandex.ru
ORCID iD: 0000-0001-7071-8067

 Ph.D.  of Medical Sciences; Senior Researcher at the laboratory of immunodiagnostic research methods at Federal State Budgetary Scientific Institution «I.Mechnikov Research Institute of Vaccines and Sera», Moscow

Russian Federation

Tatiana Zaytseva

I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow

Email: zat25@yandex.ru
ORCID iD: 0000-0001-9205-322X

Ph.D.  of Medical Sciences, Senior Lecturer at the Department of Microbiology, Virology, and Immunology at  I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow

Russian Federation

Vitaly Zverev

Federal State Budgetary Scientific Institution «I.Mechnikov Research Institute of Vaccines and Sera», Moscow; I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow

Email: vitalyzverev@outlook.com
ORCID iD: 0000-0001-5808-2246

Dr. of Biological Sciences, Professor, Academician Russian Academy of Sciences; Head of Department at the Department of Microbiology, Virology, and Immunology at  I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow; Scientific director of the Federal State Budgetary Scientific Institution «I.Mechnikov Research Institute of Vaccines and Sera», Moscow

Russian Federation

References

  1. Арзуманян В.Г., Мальбахова Е.Т., Фошина Е.П., Артемьева Т.А., Бутовченко Л.М., Вартанова Н.О., Шмелёва О.А. Патент на изобретение № 2602298 от 21.10.2016 по заявке № 2015113069, приоритет 10.04.2015: «Способ определения совокупной активности антимикробных пептидов как маркера состояния местного иммунитета различных эпителиальных тканей». Патентообладатель ФГБНУ НИИВС им. Мечникова.
  2. Арзуманян В.Г., Ожован И.М. Модифицированный метод оценки целостности цитоплазматической мембраны клеток эукариот. Бюллетень эксперим.биологии и медицины. 2002, 134(7): с. 118–120.
  3. Арзуманян В.Г., Ожован И.М., Свитич О.А. Антимикробное действие альбумина на клетки бактерий и дрожжей // БЭБМ, 2019, Т. 167, № 6, с. 722–725.
  4. Богатова О. В., Догаева Н.Г. Определение качества молока: Методические указания к лабораторному практикуму/ Оренбург: ОГУ, 2002, 39 с.
  5. Колыганова Т.И., Арзуманян В.Г., Богданова Е. А., Зверев В. В. Альтернативные методы оценки противомикробной активности сыворотки грудного молока. Бюллетень экспериментальной биологии и медицины. 2021, Том 171, № 4, С. 525–528. doi: 10.47056/0365-9615-2021-171-4-525-528.
  6. Колыганова Т.И., Арзуманян В.Г., Хорошко Н. В, Зверев В. В. Различия гуморальных факторов иммунной защиты грудного молока и молозива. Вопросы детской диетологии, 2021, Т.19, С. 1–8. doi: 10.20953/1727–5784-2021-2.
  7. Математические методы обработки данных (онлайн расчёт) https://www.psychol-ok.ru/lib/statistics.html
  8. Andersson Y, Lindquist S, Lagerqvist C, Hernell O. Lactoferrin is responsible for the fungistatic effect of human milk. Early Hum Dev. 2000, Aug;59(2):95-105. doi: 10.1016/s0378-3782(00)00086-4.
  9. Brandtzaeg P. The mucosal immune system and its integration with the mammary glands. J Pediatr. 2010 Feb;156(2 Suppl): S8-15. doi: 10.1016/j.jpeds.2009,11.014.
  10. Daristan Jamal, Akhter Ahmed, Abdulilah Ismaeil. Inhibitory Effect of Breast Milk Against Pediatric Bacterial Infection. Journal of pure and applied science salahaddin university. 2011, V 23, P.51.
  11. Funakoshi S, Doi T, Nakajima T, Suyama T, Tokuda M. Antimicrobial effect of human serum IgA. Microbiol Immunol. 1982, 26(3):227-39. doi: 10.1111/j.1348–0421. 1982.tb00174. x.
  12. Garcia-Rodenas CL, De Castro CA, Jenni R, Thakkar SK, Beauport L, Tolsa JF, Fischer-Fumeaux CJ, Affolter M. Temporal changes of major protein concentrations in preterm and term human milk. A prospective cohort study. Clin Nutr. 2019, Aug;38(4):1844-1852. doi: 10.1016/j.clnu.2018.07.016.
  13. Kavishwar, A., & Shukla, P. K. Candidacidal activity of a monoclonal antibody that binds with glycosyl moieties of proteins ofCandida albicans. Medical Mycology, 2006, 44(2), 159–167. doi: 10.1080/13693780500266038
  14. Lönnerdal B, Erdmann P, Thakkar SK, Sauser J, Destaillats F. Longitudinal evolution of true protein, amino acids and bioactive proteins in breast milk: a developmental perspective. J Nutr Biochem. 2017, Mar; 41:1-11. doi: 10.1016/j.jnutbio.2016.06.001.
  15. Lönnerdal B, Lien EL. Nutritional and physiologic significance of alpha-lactalbumin in infants. Nutr Rev. 2003, Sep;61(9):295-305. doi: 10.1301/nr.2003.sept.295-305.
  16. Montagne P, Cuillière ML, Molé C, Béné MC, Faure G. Changes in lactoferrin and lysozyme levels in human milk during the first twelve weeks of lactation. Adv Exp Med Biol. 2001, 501:241-7. doi: 10.1007/978-1-4615-1371-1_30.
  17. Nagasawa T, Kiyosawa I, Fukuwatari Y, Kitayama T, Uechi M. Alpha-lactalbumin and serum albumin in human milk. J Dairy Sci. 1973, Feb;56(2):177-80. doi: 10.3168/jds. s0022-0302(73)85142-2.
  18. Nakano M, Suzuki M, Wakabayashi H, Hayama K, Yamauchi K, Abe F, Abe S. Synergistic anti-candida activities of lactoferrin and the lactoperoxidase system. Drug Discov Ther. 2019,13(1):28-33. doi: 10.5582/ddt.2019.01010.
  19. Rai D, Adelman AS, Zhuang W, Rai GP, Boettcher J, Lönnerdal B. Longitudinal changes in lactoferrin concentrations in human milk: a global systematic review. Crit Rev Food Sci Nutr. 2014, 54(12):1539-47. doi: 10.1080/10408398.2011.642422.
  20. Shin K, Hayasawa H, Lönnerdal B. Purification and quantification of lactoperoxidase in human milk with use of immunoadsorbents with antibodies against recombinant human lactoperoxidase. Am J Clin Nutr. 2001, May;73(5):984-9. doi: 10.1093/ajcn/73.5.984.
  21. Samaranayake YH, Samaranayake LP, Wu PC, So M. The antifungal effect of lactoferrin and lysozyme on Candida krusei and Candida albicans. APMIS. 1997, Nov;105(11): p. 875–83.

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Copyright (c) 2022 Arzumanian V., Kolyganova T., Svitich O., Samoylikov P., Konanykhin S., Zaytseva T., Zverev V.

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