A complex diagnostics of early gastric cancer associated with Helicobacter pylori infection in the adult population of the Krasnoyarsk Territory

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Abstract

The aim of our study was to evaluate the clinical-anamnestic, serological, immunological and biochemical tests used for early diagnostics of gastric cancer associated with Helicobacter pylori infection in the adult population of the Krasnoyarsk Territory. Materials and methods. The control group consisted of 104 apparently healthy blood donors, the comparison group – 97 patients with chronic atrophic gastritis as well as a group of patients with early gastric cancer comprising 98 subjects. Assessment of monocyte and neutrophil spontaneous and induced chemiluminescence (CL) was carried out on a 36-channel biochemiluminometer BLM-3607. Phagocytosis was measured by using a Beckman Coulter FC 500 flow cytometer. A Varian Cary Eclipse spectrofluorometer was used to study lipid peroxidation and factors of the antioxidant defense system. Results and discussion. While studying the phagocytic arm of immunity, it was found that all patients with early gastric cancer were reported to have parameters of the maximum intensity for neutrophil spontaneous CL from 17 831 c.u. and lower, whereas induced CL reached at least 30 000 c.u. Phagocytic activity of neutrophilic granulocytes in patients with early gastric cancer was 36% or less. While studying the indicators of monocytes, it was found that spontaneous and induced CL decreased from 454 c.u. and 1186 c.u., respectively, in the patients with early gastric cancer. Monocytic activity in early gastric cancer was 34% or less. In the study of lipid peroxidation, an antioxidant defense in patients with chronic atrophic gastritis and gastric cancer had increased malondialdehyde (MDA) level. Patients with gastric cancer had decreased activity of the enzyme catalase (CAT), whereas subjects with chronic atrophic gastritis had reduced glutathione peroxidase (GPO) level. In contrast, patients with early gastric cancer were featured with increased GPO activity. We have proposed coefficients for assessing the factors of the antioxydant defence system in patients: the ratio for superoxide dismutase to catalase activity (SOD/CAT) as well as the ratio for superoxide dismutase to glutathione peroxidase activity (SOD/GPO). Conclusion. During the study, threshold values of parameters were obtained for assigning groups at high risk of developing early gastric cancer, which can be used for screening in adult population.

About the authors

O. V. Smirnova

Krasnoyarsk Research Center of the Siberian Branch of the Russian Academy of Sciences, Research Institute of Medical Problems of the North

Author for correspondence.
Email: ovsmirnova71@mail.ru

Olga V. Smirnova - PhD, MD (Medicine), Associate Professor, Head of the Laboratory of Clinical Pathophysiology, Krasnoyarsk Research Center of the Siberian Branch of the Russian Academy of Sciences, Research Institute of Medical Problems of the North.

660022, Krasnoyarsk, Partizana Zheleznyaka str., 3G.

Phone: +7 913 567-97-19.

Russian Federation

V. V. Tsukanov

Krasnoyarsk Research Center of the Siberian Branch of the Russian Academy of Sciences, Research Institute of Medical Problems of the North

Email: gastro@impn.ru

PhD, MD (Medicine), Professor, Head Researcher, Clinical Department of Adults and Children Digestive System Pathology, Krasnoyarsk Research Center of the Siberian Branch of the Russian Academy of Sciences, Research Institute of Medical Problems of the North.

Krasnoyarsk.

Russian Federation

A. A. Sinyakov

Krasnoyarsk Research Center of the Siberian Branch of the Russian Academy of Sciences, Research Institute of Medical Problems of the North

Email: sinyakov.alekzandr@mail.ru

PhD (Biology), Researcher, Laboratory of Clinical Pathophysiology, Krasnoyarsk Research Center of the Siberian Branch of the Russian Academy of Sciences, Research Institute of Medical Problems of the North.

Krasnoyarsk.

Russian Federation

O. L. Moskalenko

Krasnoyarsk Research Center of the Siberian Branch of the Russian Academy of Sciences, Research Institute of Medical Problems of the North

Email: olga_olgaol@mail.ru

PhD (Medicine), Senior Researcher, Laboratory of Clinical Pathophysiology, Krasnoyarsk Research Center of the Siberian Branch of the Russian Academy of Sciences, Research Institute of Medical Problems of the North.

Krasnoyarsk.

Russian Federation

N. G. Elmanova

Krasnoyarsk Research Center of the Siberian Branch of the Russian Academy of Sciences, Research Institute of Medical Problems of the North

Email: elm-nina@yandex.ru

Junior Researcher, Laboratory of Clinical Pathophysiology, Krasnoyarsk Research Center of the Siberian Branch of the Russian Academy of Sciences, Research Institute of Medical Problems of the North.

Krasnoyarsk.

Russian Federation

E. S. Ovcharenko

Krasnoyarsk Research Center of the Siberian Branch of the Russian Academy of Sciences, Research Institute of Medical Problems of the North

Email: liza-bet@bk.ru

Junior Researcher, Laboratory of Clinical Pathophysiology, Krasnoyarsk Research Center of the Siberian Branch of the Russian Academy of Sciences, Research Institute of Medical Problems of the North.

Krasnoyarsk.

Russian Federation

References

  1. Баранская Е.К., Ивашкин В.Т. Клинический спектр предраковой патологии желудка // Российский журнал гастроэнтерологии, гепатологии, колопроктологии. 2002. № 3. С. 7–14.
  2. Борунов Е.В., Смирнова Л.П., Щепеткин И.А. Высокая активность антиокислительных ферментов как фактор избегания контроля иммунной системы // Бюллетень экспериментальной биологии и медицины. 1989. Т. 4. C. 41–43.
  3. Ланкин В.З., Поляков В.М., Гуревич С.М. Метаболизм перекисей липидов и его регуляция в процессе роста трансплантируемых и индуцированных химическими канцерогенами злокачественных опухолей // Липиды: структура, биосинтез, превращения и функции. М.: Наука, 1977. С. 93–103.
  4. Пасечников В.Д., Чуков С.З. Эпидемиология рака желудка. Российский журнал гастроэнтерологии, гепатологии, колопроктологии. 2002. № 3. С. 18–26.
  5. Пушкина Т.А., Токаев Э.С., Попова Т.С., Бородина Е.Н. Супероксиддисмутаза в составе антиоксидантной терапии: состояние вопроса и перспективы (обзор литературы). Журнал им. Н.В. Склифосовского. 2016. № 4. С. 42–47.
  6. Реброва О.Ю. Статистический анализ медицинских данных. Применение пакета статистических программ Statistica. Москва: Медиа Сфера, 2006. 312 с.
  7. Allum W.H., Powell D.J., McConkey C.C., Fielding J.W. Gastric cancer: a 25 year review. Brit. J. Surg., 1989, vol. 76, pp. 535– 540. doi: 10.1002/bjs.1800760604
  8. Castro-Fernandez M., Vargas-Romero J. Infection with Helicobacter pylori. Prevalence, research and impact of antibiotic resistance. Rev. Esp. Enferm. Dig., 2009, vol. 101, no. 11, pp. 743–756. doi: 10.4321/s1130-01082009001100001
  9. Choi M.G., Lee J.H., Park K.J., Yang H.K., Park J.G., Lee K.U., Choe K.J., Kim J.P. Chronological changes of clinicopathologic features in gastric cancer. J. Korean. Surg. Soc., 1999, vol. 57, pp. 514–522.
  10. Correa P. Chronic gastritis as a cancer precursor. Scand. J. Gastroenterol., 1984, vol. 104, pp. 131–136.
  11. Correa P., Houghton J. Carcinogenesis of Helicobacter pylori. Gastroenterology, 2007, vol. 133, no. 2, pp. 659–672. doi: 10.1053/j.gastro.2007.06.026
  12. Fuchs C., Mayer R. Gastric Carcinoma. N. Engl. J. Med., 1995, vol. 333, no. 1, pp. 32–41. doi: 10.1056/NEJM199507063330107
  13. Jemal A., Siegel R., Ward E., Murray T., Xu J., Thun M.J. Cancer statistics, 2007. CA Cancer J. Clin., 2007, vol. 57, no. 1, pp. 43– 66. doi: 10.3322/canjclin.57.1.43
  14. Karpeh M.S., Kelsen D.P., Tepper J.E. Cancer of the stomach. In: Cancer: Principles and Practice of Oncology, 7th ed. Philadelphia: Lippincott Williams & Wilkins, 2005, pp. 791–814.
  15. Kato M., Asaka M. Recent development of gastric cancer prevention. Jpn J. Clin. Oncol., 2012, vol. 42, no. 11, pp. 987—994. doi: 10.1093/jjco/hys151
  16. Tsukuma H., Oshima A., Nakahara H., Morii T. Natural history of early gastric carcer: a non-concurrent, long term, follow up study. Gut, 2000, vol. 47, no. 5, pp. 618–621. doi: 10.1136/gut.47.5.618
  17. Uemura N., Okamoto S., Yamamoto S., Matsumura N., Yamaguchi S., Yamakido M., Taniyama K., Sasaki N., Schlemper R.J. Helicobacter pylori infection and the development of gastric cancer. N. Engl. J. Med., 2001, vol. 345, no. 11, pp. 784–789. doi: 10.1056/NEJMoa001999
  18. Wu W., Yang Y., Sun G. Recent insights into antibiotic resistance in Helicobacter pylori eradication. Gastroenterol. Res. Pract., 2012, vol. 2012: 723183. doi: 10.1155/2012/723183
  19. Yamagata H., Kiyohara Y., Aoyagi K., Kato I., Iwamoto H., Nakayama K., Shimizu H., Tanizaki Y., Arima H., Shinohara N., Kondo H., Matsumoto T., Fujishima M. Impact of Helicobacter pylori infection on gastric cancer incidence in a general Japanese population. Arch. Intern. Med., 2000, vol. 160, no. 13, pp. 1962–1968. doi: 10.1001/archinte.160.13.196

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Copyright (c) 2021 Smirnova O.V., Tsukanov V.V., Sinyakov A.A., Moskalenko O.L., Elmanova N.G., Ovcharenko E.S.

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