Neurotransmitter level in neuroamin-containing lung structures in different forms of tuberculosis

Cover Page

Cite item

Abstract

The aim of the study was to identify features of varying localization of monoamines (catecholamines, serotonin) in the cell structures in tuberculosis-affected lung autopsy samples. Material and methods. 165 cases of secondary pulmonary tuberculosis were studied by examining 2,500 histological preparations from patients aged 30 to 55 years, divided into 5 groups, corresponding to the forms of tuberculosis. The Falck–Hillarp luminescence-histochemical method was used to determine the content of neurotransmitters in lung structures. Results. While using the Falck–Hillarp luminescence-histochemical method, mycobacteria tuberculosis containing catecholamines and serotonin were detected   in autopsy material of lungs affected by a specific inflammatory process, as well as neuroamine-containing granular luminescent cells and mast cells. Affected and intact areas of the lungs contain different amounts of monoamines. The number of cells and level of the bioamines studied depends on the form of secondary tuberculosis. It was found that in small forms of secondary tuberculosis (acute focal, fibrous-focal, infiltrative), the number of cells and quantitative parameters of neurotransmitters (catecholamines, serotonin) examined in granular luminescent and mast cells change to rise. In case of lung destructive processes, the level of catecholamines in the structures studied of affected area was higher than in control. The concentration of serotonin in the studied cells lung tissue affected by the tuberculosis process was reduced. Elastic nerve fibers at the sites of mycobacteria were altered as well. They displayed no clear luminescence, looked “swollen”, sometimes lacking varicose veins. Conclusion. Comparing the results of the study with the literature data, it was found that pulmonary tuberculosis occurs as a delayed reaction, and Mycobacterium tuberculosis manifests itself as a corpuscular antigen.

About the authors

N. E. Gimaldinova

Chuvash State University named after I.N. Ulyanov

Author for correspondence.
Email: ngimaldinova@yandex.ru
ORCID iD: 0000-0003-2475-3392

Natalia E. Gimaldinova - PhD (Medicine), Associate Professor, Department of General and Clinical Morphology and Forensic Medicine, Chuvash State University named after I.N. Ulyanov.

428031, Cheboksary, Moskovsky pr., 15.

Phone: +7 927 852-34-16.

Russian Federation

L. A. Lyubovtseva

Chuvash State University named after I.N. Ulyanov

Email: rufer06@rambler.ru
ORCID iD: 0000-0002-2818-568X

PhD, MD (Biology), RANH Corresponding Member, Professor, Department of General and Clinical Morphology and Forensic Medicine, Chuvash State University named after I.N. Ulyanov.

Cheboksary.

Russian Federation

E. V. Lyubovtseva

Republican Bureau of Forensic Medical Examination of the Ministry of Health of Chuvashia

Email: lybovceva@gmail.ru
ORCID iD: 0000-0002-6714-854X

PhD, MD (Medicine), Professor, Head of the Department of Scientific Technologies and Innovations, Republican Bureau of Forensic Medical Examination of the Ministry of Health of Chuvashia.

Cheboksary.

Russian Federation

References

  1. Бережная Н.М., Сепиашвили Р.И. Тучные клетки и гистамин: физиологическая роль // Аллергология и иммунология. 2003. Т. 4, № 3. С. 29–38.
  2. Богатых С.П., Любовцева Л.А. Динамика содержания нейромедиаторов в структурах легких крыс в норме и после экспериментального воздействия природным газом // Современные проблемы науки и образования. 2012. Т. 5. С. 37–41.
  3. Воробьева О.В., Любовцева Л.А., Гурьянова Е.А. Серотонинсодержащие клетки в первичном органе кроветворения после аутологичной пересадки костного мозга // Бюллетень экспериментальной биологии и медицины. 2019. Т. 168, № 9. С. 355–358.
  4. Гималдинова Н.Е., Любовцева Л.А., Гималдинов Р.Ф., Воробьева О.В. Влияние циклоферона на распределение нейроаминов в биоаминсодержащих структурах селезенки // Вестник новых медицинских технологий. 2018. Т. 25, № 3. С. 101–106.
  5. Диндяев С.В., Виноградов С.Ю., Погорелов Ю.В., Торшилова И.Ю., Параскун А.А. Флуоресцентно-гистохимическое выявление катехоламинов и серотонина // Актуальные проблемы биологии, медицины и экологии. 2004. Т. 4, № 1. С. 84–85.
  6. Любовцева Л.А., Воробьева О.В. Характеристика биоаминсодержащих клеток при введении собственного костного мозга // Медицина и образование в Сибири: сетевое научное издание. 2015. № 4. URL: https://www.ngmu.ru/cozo/mos/article/annotacy_eng.php?id=1871
  7. Любовцева Е.В., Любовцева Л.А. Динамика нейромедиаторов в костном мозге крыс после иглоукалывания в точке гипоталамуса // Теоретическая и практическая медицина. 2010. Т. 8. С. 48–50.
  8. Любовцева Л.А., Тихонова Н.Н., Яшина Н.Е. Биоаминсодержащие структуры тимуса и костного мозга крыс при аутомиелотрансплантации // Морфология. 2006. Т. 129, № 2. С. 55–56.
  9. Мотавкин П.А. Клиническая и экспериментальная патофизиология легких // М.: Наука, 1998. 366 с.
  10. Яглов В.В. Биология диффузной эндокринной системы. М.: МГАВМИБ, 1995. 32 с.
  11. Falck B., Hillarp N.A., Thieme G., Torp A. Fluorescence of catecholamines and related compounds condensed with formaldehyde. J. Histochem. Citochem., 1962, vol. 10, pp. 348–354.

Supplementary files

There are no supplementary files to display.


Copyright (c) 2021 Gimaldinova N.E., Lyubovtseva L.A., Lyubovtseva E.V.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies