THE RAPID CULTURE METHOD FOR THE INDICATION OF RABIES VIRUS ANTIGEN IN INFECTED CELL CULTURES

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Abstract. The rapid culture method for the indication of antigens of rabies virus has been improved. It allows to detect the antigen of rabies virus within 5 hours from the moment the cell cultures was infected. To achieve this aim it is necessary to use cell culture BHK-F, polyclonal antirabies FITC labelled immunoglobulin and direct method of membrane immunofluorescense. Applying of twofold freshly filtrated 3% paraformaldehyde as fixing solution allows to detect early virus proteins of rabies virus.

About the authors

E. P. Barkova

Mechnicov Research Institute of Vaccines and Sera, RAMS, Moscow

Email: firaya77@gmail.com

PhD (Biology), Leading Researcher, Laboratory of Hybrid Cell Cultures, Department of Virology

Russian Federation

F. G. Nagieva

Mechnicov Research Institute of Vaccines and Sera, RAMS, Moscow

Author for correspondence.
Email: firaya77@gmail.com

PhD, MD (Medicine), Head of the Laboratory of Hybrid Cell Cultures, Department of Virology

115088, Russian Federation, Moscow, 1st Dubrovskaya str., 15

Russian Federation

V. G. Nikulina

Mechnicov Research Institute of Vaccines and Sera, RAMS, Moscow

Email: firaya77@gmail.com
PhD (Medicine), Leading Researcher, Laboratory of Hybrid Cell Cultures, Department of Virology Russian Federation

A. N. Lisakov

Mechnicov Research Institute of Vaccines and Sera, RAMS, Moscow

Email: firaya77@gmail.com
Junior Researcher, Laboratory of Hybrid Cell Cultures, Department of Virology Russian Federation

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Copyright (c) 2014 Barkova E.P., Nagieva F.G., Nikulina V.G., Lisakov A.N.

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