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Abstract. The aim of the study is evaluation of links between presence in blood of specific pre-existing IgG to respiratory-syncytial virus (RSV), clinical course of RSV infection and character specific to RSV humoral immune response in patients of different ages. The antibodies were detected by ELISA using whole RS virus or synthetic peptides corresponded to the selected determinants of the envelope RSV proteins. It was shown that RS specific maternal IgG antibodies passively transferred to babies in utero can circulate in the blood up to 10 months of life. The analysis of paired sera of 45 babies in the age of 1–10 months revealed firstly that presence of maternal IgG specific antibodies to the conservative B-cell immunogenic determinants of the F-protein (amino acids 221–232) and/or the G-protein (amino acids 152–164 and 184–198) is coupled with more high morbidity of primary RSV infection (89% versus 56%, p = 0.023), and also with more high frequency of complicated by bronchus obstruction course of the disease (81% versus 20%, р = 0.001) in compare with babies who were serologically negative to the maternal determinants specific antibodies. The correlation analysis has shown that the high presence of maternal determinant-specific IgG in the blood in babies till 10 months of life is associated in the case of primary infection with disbalance of humoral anti-viral immune response: intensive synthesis of serum RSV IgA. This is evidence of complicated course of infection with simultaneous suppression of response to RSV specific IgG. As opposed to the primary RSV infection in patients older than 3 years (n = 121) it was not detected links between anamnestic determinant-specific IgG synthesized by own immune system as the results of previous disease episodes and synthesis of anti-RSV IgG, IgM, IgE and IgA in RSV re-infections. In the contrast to babies in more older patients the feedback connection between level of pre-existing determinant-specific “memory IgG” and diagnostics of RSV infection has been established.

About the authors

V. Z. Krivitskaya

ФГБУ НИИ гриппа Минздравсоцразвития России, Санкт-Петербург

Author for correspondence.

к.б.н., ведущий научный сотрудник лаборатории биотехнологии диагностических препаратов

197376, Санкт-Петербург, ул. Проф. Попова, 15/17

Russian Federation

E. M. Voitsehovskaya

ФГБУ НИИ гриппа Минздравсоцразвития России, Санкт-Петербург

Russian Federation

V. F. Suhovetskaya

ФГБУ НИИ гриппа Минздравсоцразвития России, Санкт-Петербург

Russian Federation

E. G. Golovachova

ФГБУ НИИ гриппа Минздравсоцразвития России, Санкт-Петербург

Russian Federation

N. I. Lvov

ФГКВОУ ВПО Военно-медицинская академия им. С.М. Кирова Министерства обороны Российской Федерации, Санкт-Петербург

Russian Federation

A. A. Sominina

ФГБУ НИИ гриппа Минздравсоцразвития России, Санкт-Петербург

Russian Federation


  1. Кривицкая В.З., Александрова Н.И., Орлов А.В., Походзей И.В. Реакция иммуноглобулина А на респираторно-синцитиальную вирусную инфекцию у детей и взрослых с различными формами острого и хронического бронхита // Иммунология. — 1999. — № 2. — С. 51–55.
  2. Кривицкая В.З., Соминина А.А., Власов Г.П., Суховецкая В.Ф., Голованова А.К. Возрастные особенности сайт-направленного иммунитета при респираторно-синцитиальной вирусной инфекции // Вопросы вирусологии. — 2004. — Т. 49, № 4. — С. 25–29.
  3. Кривицкая В.З, Соминина А.А., Суховецкая В.Ф., Милькинт К.К, Сверлова М.В. Иммунопатологический аллергический Th2-тип противовирусного гуморального иммунного ответа у детей с респираторно-синцитиальной вирусной инфекцией // Цитокины и воспаление. — 2004. — Т. 3, № 3. — С. 34–41.
  4. Кривицкая В.З., Сверлова М.В., Суховецкая В.Ф., Львов Н.И., Васильева И.A., Соминина А.А. Выявление аутореактивных антител класса М у пациентов с респираторными вирусными инфекциями // Медицинская иммунология. — 2008. — Т. 10, № 2–3. — С. 229–238.
  5. Aberle J.H., Aberle S.W., Dworzak M.N., Mandl C.W., Rebhandl W., Vollnhofer G., Kundi M., Popow-Kraupp T. Reduced interferon-gamma expression in peripheral blood mononuclear cells of infants with severe respiratory syncytial virus disease // Am. J. Respir. Crit. Care Med. — 1999. — Vol. 160. — P. 1263–1268.
  6. Brown W.R. Relationships between immunoglobulins and the intestinal epithelium // Gastroenterology. — 1978. — Vol. 75, N 1. — Р. 129–138.
  7. Dakhama A., Lee Y.M., Ohnishi H., Jing X., Balhorn A., Takeda K., Gelfand E.W. Virus-specific IgE enhances airway responsiveness on reinfection with respiratory syncytial virus in newborn mice // J. Allergy Clin. Immunol. — 2009. — Vol. 123, N 1. — Р. 138 –145. e5.
  8. Falsey A.R., Singh H.K., Walsh E.E. Serum antibody decay in adults following natural respiratory syncytial virus infection // J. Med. Virol. — 2006. — Vol. 78, N 11. — Р. 1493–1497.
  9. Getahun A., Heyman B. Studies on the mechanism by which antigen-specific IgG suppresses primary antibody responses: evidence for epitope masking and decreased localization of antigen in the spleen // Scand. J. Immunol. — 2009. — Vol. 70, N 3. — Р. 277–287.
  10. Glezen W.P., Paredes A., Allison J.E., Taber L.H., Frank A.L. Risk of respiratory syncytial virus infection for infants from low income families in relationship to age, sex, ethnic group, and maternal antibody level // J. Pediatr. — 1981. — Vol. 98. — Р. 708 –715.
  11. Hacimustafaoglu M., Celebi S., Aynaci E., Sinirtas M., Koksal N., Kucukerdogan A., Ercan I., Goral G., Ildirim I. The progression of maternal RSV antibodies in the offspring // Arch. Dis. Child. — 2004. — Vol. 89, N 1. — Р. 52–53.
  12. Hägglund S., Hu K.F., Larsen L.E., Hakhverdyan M., Valarcher J.F., Taylor G., Morein B., Belák S., Alenius S. Bovine respiratory syncytial virus ISCOMs — protection in the presence of maternal antibodies // Vaccine. — 2004. — Vol. 23, N 5. — Р. 646–655.
  13. Heyman B. Regulation of antibody responses via antibodies, complement, and Fc receptors // Annu. Rev. Immunol. — 2000. — Vol. 18. — P. 709–737.
  14. Hjelm F., Carlsson F., Getahun A., Heyman B. Antibody-mediated regulation of the immune response // Scand. J. Immunol. — 2006. — Vol. 64, N 3. — Р. 177–184.
  15. Okiro E.A., Sande C., Mutunga M., Medley G.F., Cane P.A., Nokes D.J. Identifying infections with respiratory syncytial virus by using specific immunoglobulin G (IgG) and IgA enzyme-linked immunosorbent assays with oral-fluid samples // J. Clin. Microbiol. — 2008. — Vol. 46, N 5. — Р. 1659–1662.
  16. Plotnicky-Gilquin H., Cyblat-Chanal D., Aubry J.P., Champion T., Beck A., Nguyen T., Bonnefoy J.Y., Corvaïa N. Gamma interferon-dependent protection of the mouse upper respiratory tract following parenteral immunization with a respiratory syncytial virus G protein fragment // J. Virol. — 2002. — Vol. 76, N 20. — Р. 10203–10210.
  17. Power U.F., Plotnicky-Gilquin H., Goetsch L., Champion T., Beck A., Haeuw J.F., Nguyen T.N., Bonnefoy J.Y., Corvaïa N. Identification and characterisation of multiple linear B cell protectopes in the respiratory syncytial virus G protein protein // Vaccine. — 2001 — Vol. 19, N 17–19. — P. 2345–2351.
  18. Queiroz D., Durigon E.L., Botosso V.F., Ejzemberg B., Vieira S.E., Mineo J.R., Yamashita C., Hein N., Lopes C.L., Cacharo A.L., Stewien K.E. Immune response to respiratory syncytial virus in young Brazilian children // Braz. J. Med. Biol. Res. — 2002. — Vol. 35, N 10. — P. 1183–1193.
  19. Ravetch J.V., Nimmerjahn F. Fc receptors and their role in immune regulation and inflammation // Fundamental Immunology. — Ed. Paul W.E. — 6th edition. — Lippincott, Williams and Wilkins, 2008. — Chapter 22. — P. 685–705.
  20. Routledge E.G., Willcocks M.M., Samson A.C., Morgan L., Scott R., Anderson J.J., Toms G.L. The purification of four respiratory syncytial virus proteins and their evaluation as protective agents against experimental infection in BALB/c mice // J. Gen. Virol. — 1988. — Vol. 69. — P. 293–303.
  21. Siegrist C.A. Mechanisms by which maternal antibodies influence infant vaccine responses: review of hypotheses and definition of main determinants // Vaccine. — 2003. — Vol. 21. — P. 3406–3412.
  22. Trudel M., Nadon F., Seguin C., Dionne G., Lacroix M. Identification of a synthetic peptide as a part of major neutralization epitope of respiratory syncytial virus // J. Gen. Virology. — 1987. — Vol. 68. — P. 2273–2280.
  23. Vangeel I., Antonis A.F., Fluess M., Riegler L., Peters A.R., Harmeyer S.S. Efficacy of a modified live intranasal bovine respiratory syncytial virus vaccine in 3-week-old calves experimentally challenged with BRSV // Vet. J. — 2007. — Vol. 174, N 3. — P. 627–635.

Copyright (c) 2014 Krivitskaya V.Z., Voitsehovskaya E.M., Suhovetskaya V.F., Golovachova E.G., Lvov N.I., Sominina A.A.

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