Russian Journal of Infection and ImmunityRussian Journal of Infection and Immunity2220-76192313-7398SPb RAACI45410.15789/2220-7619-2016-4-395-398UnknownRAPID URINE SCREENING FOR BACTERIURIA IN CHILDREN USING MICROBIOLOGY ANALYZER, COMBINING PHOTOMETRIC AND COHERENT FLUCTUATION NEPHELOMETRIC METHODSGur’evA. S.Master (Physics and Mathematics), Researcher, Laboratory of Nanotechnology in Medicine, Department of Biophysics, Federal Research and Clinical Center of Physical-Chemical Medicine; Researcher, Medtechnopark LTD, Moscow, Russian Federationcoherneph@mail.ruKuznetsovaO. Yu.Master of Science in Biotechnology, Researcher, Laboratory Of Nanotechnology, Department Of New Technologycoherneph@mail.ruPyasetskayaM. F.Bacteriologist, Clinical Diagnostic Laboratorycoherneph@mail.ruSmirnovaI. A.Bacteriologist, Clinical Diagnostic Laboratorycoherneph@mail.ruBelyaevaN. A.Bacteriologist, Clinical Diagnostic Laboratorycoherneph@mail.ruVerbovV. N.PhD (Chemistry), Head of the Department of New Technologycoherneph@mail.ruVolkovA. Yu.PhD (Physics and Mathematics), Senior Researcher, Laboratory of Nanotechnology in Medicine, Department of Biophysics, Federal Research and Clinical Center of Physical-Chemical Medicine; Director of Medtechnopark LTD, Moscow, Russian Federationcoherneph@mail.ruFederal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russian Federation
St. PetersburgPasteur Institute of Epidemiology and Microbiology, St. Petersburg, Russian FederationSt. PetersburgPasteur Institute of Epidemiology and Microbiology, St. Petersburg, Russian FederationN.F. Filatov Children’s City Clinical Hospital No. 5, St. Petersburg, Russian FederationFederal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russian Federation
Medtechnopark LTD, Moscow, Russian Federation06012017643953980601201706012017Copyright © 2017, Gur’ev A.S., Kuznetsova O.Y., Pyasetskaya M.F., Smirnova I.A., Belyaeva N.A., Verbov V.N., Volkov A.Y.2017<p>One of the most widespread microbiological analyses in clinical practice is urine culture, and up to 80% of urine samples turn out to be negative. In this study we demonstrate applicability of coherent fluctuating nephelometry (CFN) method for rapid urine screening and negative samples identification for the purpose of reducing the workload of microbiological laboratory. 205 urine samples were tested by conventional culture method (CCM) and using CFN-analyzer. The agreement between CCM and CFN was 87.8%. Compared to CCM, CFN demonstrated sensitivity 94.5%, specificity 85.3%, negative predictive value 97.7%, positive predictive value 70.3%. In this study CFN-analyzer allowed to identify 85.3% of negative urine samples (63.9% of all tested samples) within 4 hours, and 94.5% of positive samples were retained for later CCM. Moreover none of samples with count 104 CFU/ml was omitted.</p>
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