Russian Journal of Infection and ImmunityRussian Journal of Infection and ImmunityИнфекция и иммунитет2220-76192313-7398SPb RAACI2810.15789/2220-7619-2011-1-59-66REVIEWSОБЗОРЫOPPORTUNITIES OF FLOW CYTOMETRY IN DIAGNOSTICS OF INFECTIOUS DISEASES. PART 1ВОЗМОЖНОСТИ ПРОТОЧНОЙ ЦИТОФЛЮОРИМЕТРИИ В ДИАГНОСТИКЕ ИНФЕКЦИОННЫХ ЗАБОЛЕВАНИЙ. Часть 1KhaidukovS. V.ХайдуковС. В.Russian Federation
д.б.н., зав. лабораторией физиологии и патологии иммунной системы
Abstract. Development of a modern medical science demands more thorough and deeper approach to diagnostics of diseases. New diagnostic technologies for these investigations are necessary and flow cytometry is such technology. Modern achievements in the field of fluorescent dyes, development of laser and computer technologies, have led to wide use of the given technology in medical practice. Infectious immunology is not exception. Cytokines production analysis at tuberculosis, HLA-DR expression changes for monocytes and CD64 for granulocytes at sepsis are the important diagnostic attributes. Flow cytometry offers new approaches to early and faster diagnostics of infringements of immune system at tuberculosis and sepsis, allows supervising development of these pathologies more effectively and estimate efficiency of therapy.
Резюме. Развитие современной медицинской науки требует более досконального и более глубокого подхода к диагностике заболеваний, и как следствие этого возникает необходимость в применении новых диагностических технологий. Проточная цитофлюориметрия является такой технологией. Использование современных достижений в области флуоресцентных красителей, развитие лазерных и компьютерных технологий, привели к широкому использованию данной технологии в медицинской практике. Не является исключением и инфекционная иммунология. Использование анализа продукции цитокинов при туберкулезе и изменение экспрессии HLA-DR на моноцитах и CD64 на гранулоцитах при сепсисе являются важными диагностическими признаками. Проточная цитофлюориметрия предлагает новые подходы к ранней и более быстрой диагностике нарушений иммунной системы при туберкулезе и сепсисе, позволяет более эффективно контролировать развитие этих патологий и оценить эффективность терапии.
flow cytometrytuberculosisCD27sepsismonocytesneutrophilsCD64проточная цитометриятуберкулезCD27сепсисмоноцитынейтрофилыCD641. Зурочка А.В., Котляров А.Н., Кувайцев М.В., Квятковская С.В., Зурочка В.А., Рябова Л.В., Хайдуков С.В. Изменения Экспрессии HLA-DR-антигенов на моноцитах у детей и ее клиническая значимость при сепсисе // Медицинская иммунология. — 2008. — Т. 10, № 4–5. — С. 379–388.2. Хайдуков С.В., Зурочка А.В. Проточная цитометрия как современный метод анализа в биологии и медицине // Медицинская иммунология. — 2007. — Т. 9, № 4–5. — С. 373–378.3. Хайдуков С.В. Подходы к стандартизации метода проточной цитометрии для иммунофенотипирования. Настройка цитометров и подготовка протоколов для анализа // Медицинская иммунология. — 2007. — Т. 9, № 6. — С. 569–574.4. Asadullah K., Woiciechowsky C., Docke W.D., Egerer K., Kox W., Volgel S., Sterry W., Volk H.D. Very low monocytic HLA-DR expression indicates high risk of infection — immunomonitoring for patients after neurosurgery and patients during high dose steroid therapy // Eur. J. Emerg. Med. — 1995. — Vol. 2. — P. 184 –190.5. Bizzarro M.J., Raskind C., Baltimore R.S., Gallagher P.G. Seventy five years of neonatal sepsis at Yale: 1928–2003 // Pediatrics. — 2005. — Vol. 116. — P. 595–602.6. Boelens P.G., Houdijk A.P., Fonk J.C., Nijveldt R.J., Ferwerda C.C., Von Blomberg-Van Der Flier B.M., Thijs L.G., Haarman H.J., Puyana J.C., Van Leeuwen P.A. Glutamine-enriched enteral nutrition increases HLA-DR expression on monocytes of trauma patients // J. Nutr. — 2002. — Vol. 132, N 9. — P. 2580–2586.7. Clark R.H., Bloom B.T., Spitzer A.R., Gerstmann D.R. Empiric use of ampicillin and cefotaxime, compared with ampicillin and gentamicin, for neonates at risk for sepsis is associated with an increased risk of neonatal death // Pediatrics. — 2006. — Vol. 117. — P. 67–74.8. Cohen G.B., Gandhi R.T., Davis D.M., Mandelboim O., Chen B.K., Strominger J.L., Baltimore D. The selective downregulation of class I major histocompatibility complex proteins by HIV-1 protects HIV-infected cells from NK cells // Immunity. — 1999. — Vol. 10. — P. 661– 671.9. Collins K.L., Chen B.K., Kalams S.A., Walker B.D., Baltimore D. HIV-1 Nef protein protects infected primary cells against killing by cytotoxic T lymphocytes // Nature. — 1998. — Vol. 391. — P. 397–401.10. Daftary A., Padayatchi N., Padilla M. HIV testing and disclosure: a qualitative analysis of TB patients in South Africa // AIDS Care. — 2007. — Vol. 19. — P. 572–577.11. Ditschkowski M., Kreuzfelder E., Rebmann V., Ferencik S., Majetschak M., Schmid E.N., Obertacke U., Hirche H., Schade U.F., Grosse-Wilde H. HLA-DR expression and soluble HLA-DR levels in septic patients after trauma // Ann. Surg. — 1999. — Vol. 229, N 2. — P. 24 6 –254.12. Docke W.D., Hoflich C., Davis K.A., Rottgers K., Meisel C., Kiefer P., Weber S.U., Hedwig-Geissing M., Kreuzfelder E., Tschentscher P., Nebe T., Engel A., Monneret G., Spittler A., Schmolke K., Reinke P., Volk H.D., Kunz D. Monitoring temporary immunodepression by flow cytometric measurement of monocytic HLA-DR expression: a multicenter standardized study // Clin. Chem. — 2005. — Vol. 51, N 12. — P. 2341–2347.13. Fine P.E., Rodrigues L.C. Modern vaccines. Mycobacterial diseases // Lancet. — 1990. — Vol. 335. —P. 1016 –1020.14. Flynn J.L., Chan J. Immunology of tuberculosis // Annu. Rev. Immunol. — 2001. — Vol. 19. — P. 93–129.15. Fumeaux T., Pugin J. Role of interleukin-10 in the intracellular sequestration of human leukocyte antigen-DR in monocytes during septic shock // Am. J. Respir. Crit. Care. Med. — 2002. — Vol. 166, N 11. — P. 1475–1482.16. Garcia P., Llano M., de Heredia A.B., Willberg C.B., Caparrós E., Aparicio P., Braud V.M., López-Botet M. Human T cell receptor-mediated recognition of HLA-E // Eur. J. Immunol. — 2002. — Vol. 32. — P. 936—944.17. Gerdes J.S. Clinicopathologic approach to the diagnosis of neonatal sepsis // Clin. Perinatol. — 1991. — Vol. 18. — P. 361–381.18. Gonzalez B.E., Mercado C.K., Johnson L., Brodsky N.L., Bhandari V. Early markers of late-onset sepsis in premature neonates: clinical, hematological and cytokine profile // J. Perinat. Med. — 2003. — Vol. 31. — P. 60–68.19. Grimsley C., Ober C. Population genetic studies of HLA-E: evidence for selection // Hum. Immunol. — 1997. — Vol. 52. — P. 33–40.20. Guillou P.J. Biological variation in the development of sepsis after surgery or trauma // Lancet. — 1993. — Vol. 342. — P. 217–220.21. Haveman J.W., Berg A.P. van den, Berk J.M. van den, Mesander G., Slooff M.J., Leij L.H. de, The T.H. Low HLA-DR expression on peripheral blood monocytes predicts bacterial sepsis after liver transplantation: relation with prednisolone intake // Transpl. Infect. Dis. — 1999. — Vol. 1, N 3. — P. 146–152.22. Heinzel A.S., Grotzke J.E., Lines R.A., Lewinsohn D.A., McNabb A.L., Streblow D.N., Braud V.M., Grieser H.J., Belisle J.T., Lewinsohn D.M. HLA-E-dependent presentation of Mtb-derived antigen to human CD8+T cells // J. Exp. Med. — 2002. — Vol. 196. — P. 1473–1481.23. Hellberg S., Sjostrom M., Skagerberg B., Wold S. Peptide quantitative structure-activity relationships, a multivariate approach // J. Med. Chem. — 1987. — Vol. 30. — P. 1126–1135.24. Hershman M.J., Cheadle W.G., Wellhausen S.R., Davidson P.F., Polk S.C. Monocyte HLA-DR antigen expression characterizes clinical outcome in the trauma patient // Br. J. Surg. — 1990. — Vol. 77. — P. 204–207.25. Hoffman J.A., Weinberg K.I., Azen C.G., Horn M.V., Dukes L., Starnes V.A., Woo M.S. Human leukocyte antigen-DR expression on peripheral blood monocytes and the risk of pneumonia in pediatric lung transplant recipients // Transpl. Infect. Dis. — 2004. — Vol. 6. — P. 147–155.26. Hoffmeyer F., Witte K., Schmidt R.E. The high-affinity FcγRI on PMN: regulation of expression and signal transduction // Immunology. — 1997 — Vol. 92. — P. 54 4 –552.27. Hurst M.K., Yoder B.A. Detection of bacteremia in young infants: is 48 hours adequate? // Pediatr. Infect. Dis. J. — 1995. — Vol. 14. — P. 711–713.28. Jardine L., Davies M.W., Faoagali J. Incubation time required for neonatal blood cultures to become positive // J. Paediatr. Child. Health. — 2006. — Vol. 42. — P. 797–802.29. Kaufmann S.H. Envisioning future strategies for vaccination against tuberculosis // Nat. Rev. Immunol. — 2006. — Vol. 6. — P. 699–704.30. Kawasaki T., Ogata M., Kawasaki C., Tomihisa T., Okamoto K., Shigematsu A. Surgical stress induces endotoxin hyporesponsiveness and an early decrease of monocyte mCD14 and HLA-DR expression during surgery // Anesth. Analg. — 2001. — Vol. 92, N 5. — P. 1322–1326.31. Khaidukov S.V., Komaleva R.L., Nesmeyanov V.A. N-acetylglucosamine-containing muramyl peptides directly affect macrophages // Int. J. Immunopharmacol. — 1995. — Vol. 17, N 11. — P. 903–911.32. Khassawneh M., Hayajneh W.A., Kofahi H., Khader Y., Amarin Z., Daoud A. Diagnostic markers for neonatal sepsis: comparing C-reactive protein, interleukin-6 and immunoglobulin M // Scand. J. Immunol. — 2007. — Vol. 65. — P. 171–175.33. Kox W.J., Volk T., Kox S.N., Volk H.D. Immunomodulatory therapies in sepsis // Int. Care. Med. — 2000. — Vol. 26. — P. 124–128.34. Kristiansen M., Graversen J.H., Jacobsen C., Sonne O., Hoffman H.J., Law S.K., Moestrup S.K. Identification of the haemoglobin scavenger receptor // Nature. — 2001. — Vol. 409, N 6817. — P. 198–201.35. Kunz D., Pross M., Lippert H., Konig W., Manger T. Diagnostic relevance of procalcitonin, IL-6 and cellular immune status in the early phase after liver transplantation // Transplant. Proc. — 1998. — Vol. 30. — P. 23 –24.36. Lawn J.E., Wilczynska-Ketende K., Cousens S.N. Estimating the causes of 4 million neonatal deaths in the year 2000 // Int. J. Epidemiol. — 2006. — Vol. 35. —P. 70 6 –718.37. Le Tulzo Y., Pangault C., Amiot L., Guilloux V., Tribut O., Arvieux C., Camus C., Fauchet R., Thomas R., Drénou B. Monocyte human leukocyte antigen-DR transcriptional downregulation by cortisol during septic shock // Am. J. Respir. Crit. Care. Med. — 2004. — Vol. 169, N 10. — P. 114 4 –1151.38. Lin R.Y., Astiz M.E., Saxon J.C., Rackow E.C. Altered leukocyte immunophenotypes in septic shock. Studies of HLA-DR, CD11b, CD14, and IL-2R expression // Chest. — 1993. — Vol. 104, N 3. — P. 847–853.39. Lyadova I.V., Oberdorf S., Kapina M.A., Apt A.S., Swain S.L., Sayles P.C. CD4 T cells producing IFN-gamma in the lungs of mice challenged with mycobacteria express a CD27-negative phenotype // Clin. Exp. Immunol. — 2004. — Vol. 138. — P. 21–29.40. Mehr S., Doyle L.W. Cytokines as markers of bacterial sepsis in newborn infants: a review // Pediatr. Infect. Dis J. — 2000. — Vol. 19. — P. 879–887.41. Monneret G., Elmenkouri N., Bohe J., Debard A.L., Gutowski M.C., Bienvenu J., Lepape A. Analytical requirements for measuring monocytic human lymphocyte antigen DR by flow cytometry: application to the monitoring of patients with septic shock // Clin. Chem. — 2002. — Vol. 48, N 9. — P. 1589–1592.42. Ng P.C., Lam H.S. Diagnostic markers for neonatal sepsis // Curr. Opin. Pediatr. — 2006. — Vol. 18. — P. 125–131.43. Ng P.C., Li G., Chui K.M., Chu W.C., Li K., Wong R.P., Fok T.F. Quantitative measurement of monocyte HLA-DR expression in the identification of early-onset neonatal infection // Biol. Neonate. — 2006. — Vol. 89, N 2. — P. 75–81.44. Noursadeghi M., Katz D.R., Miller R.F. HIV-1 infection of mononuclear phagocytic cells: the case for bacterial innate immune deficiency in AIDS // Lancet. Infect. Dis. — 2006. — Vol. 6. — P. 794–804.45. Ottenhoff T.H. Overcoming the global crisis: ‘‘yes, we can’’, but also for TB …? // Eur. J. Immunol. — 2009. — Vol. 39. — P. 2014–2020.46. Pietra G., Romagnani C., Falco M., Vitale M., Castriconi R., Pende D., Millo E., Anfossi S., Biassoni R., Moretta L., Mingari M.C. The analysis of the natural killer-like activity of human cytolytic T lymphocytes revealed HLA-E as a novel target for TCR alpha/beta-mediated recognition // Eur. J. Immunol. — 2001. — Vol. 31. — P. 3687–3693.47. Pitton C., Fitting C., Deuren M. van, Meer J.W. van der, Cavaillon J.M. Different regulation of TNF-α and IL-1ra synthesis in LPS-tolerant human monocytes // Prog. Clin. Biol. Res. — 1995. — Vol. 392. — P. 523–528.48. Richter A., Nebe T., Wendl K., Schuster K., Klaebisch G., Quintel M., Lorenz D., Post S., Trede M. HLA-DR Expression in acute pancreatitis // Eur. J. Surg. — 1999. — Vol. 165. — P. 947–951.49. Sachse C., Prigge M., Cramer G., Pallua N., Henkel E. Association between reduced human leukocyte antigen (HLA-DR) expression on blood monocytes and increased plasma level of interleuk in-10 in patients with severe bur ns // Clin. Chem. Lab. Med. — 1999. — Vol. 37. — P. 193–198.50. Sandberg M., Eriksson L., Jonsson J., Sjostrom M., Wold S. New chemical descriptors relevant for the design of biologically active peptides. A multivariate characterization of 87 amino acids // J. Med. Chem. — 1998. — Vol. 41. — P. 2481–2491.51. Spittler A., Winkler S., Gotzinger P., Oehler R., Willheim M., Tempfer C., Weigel G., Fugger R., Boltz-Nitulescu G., Roth E. Influence of glutamine on the phenotype and function of human monocytes // Blood. — 1995. — Vol. 86, N 4. — P. 1564–1569.52. Streitz M., Tesfa L., Yildirim V., Yahyazadeh A., Ulrichs T., Lenkei R., Quassem A., Liebetrau G., Nomura L., Maecker H., Volk H.D., Kern F. Loss of receptor on tuberculin-reactive T-cells marks active pulmonary tuberculosis // PloS. One. — 2007. — Vol. 2, N 1. — e735.53. Strohmeyer J.C., Blume C., Meisel C., Doecke W.D., Hummel M., Hoeflich C., Thiele K., Unbehaun A., Hetzer R., Volk H.D. Standardized immune monitoring for the prediction of infections after cardiopulmonary bypass surgery in risk patients // Cytometry. — 2003. — Vol. 53. — P. 54–62.54. Tschaikowsky K., Hedwig-Geissing M., Schiele A., Bremer F., Schywalsky M., Schuttler J. Coincidence of pro- and anti-inflammatory responses in the early phase of severe sepsis: longitudinal study of mononuclear histocompatibility leukocyte antigen DR expression, procalcitonin, C-reactive protein, and changes in T-cell subsets in septic and postoperative patients // Crit. Care. Med. — 2002. — Vol. 30. — P. 1015–1023.55. Turner D., Hammerman C., Rudensky B., Schlesinger Y., Schimmel M.S. The role of procalcitonin as a predictor of nosocomial sepsis in preterm infants // Acta. Paediatr. — 2006. — Vol. 95. — P. 1571–1576.56. Volk H.D., Lohmann T., Heym S., Golosubow A., Ruppe U., Reinke P. et al. Decrease of the proportion of HLA-DR-monocytes as prognostic parameter for the clinical outcome of septic disease // Immunotherapeutic prospects of infectious diseases; Masihi K.N., Lange W., eds. — Heidelberg: Springer Verlag, 1990. — P. 297–301.57. Whittaker E., Kampmann B. Perinatal tuberculosis: new challenges in the diagnosis and treatment of tuberculosis in infants and the newborn // Early. Hum. Dev. — 2008. — Vol. 84. — P. 795–799.58. Wolk K., Docke W.D., von Baehr R., Volk H.D., Sabat R. Comparison of monocyte functions after LPS- or IL-10-induced reorientation: importance in clinical immunoparalysis // Pathobiology. — 1999. — Vol. 67. — P. 253–256.59. Wright S.D., Ramos R.A., Tobias P.S., Ulevitch R.J., Mathison J.C. CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein // Science. — 1990. — Vol. 249, N 4975. — P. 1431–1433.60. Zuwała-Jagiełło J. Haemoglobin scavenger receptor: function in relation to disease // Acta. Biochim. Pol. — 2006. — Vol. 53, N 2. — P. 257–268.